Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,665 T→A 18.6% G1041G (GGT→GGA narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6650TA18.6% 152.2 / 30.9 70G1041G (GGT→GGAnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (31/26);  new base A (5/8);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

TGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTC  >  minE/834596‑834732
                                                                     |                                                                   
tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtc                                                                    >  1:2139387/1‑71 (MQ=255)
  aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt                                                                  <  1:552425/71‑1 (MQ=255)
  aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt                                                                  <  1:1873440/71‑1 (MQ=255)
   tcacGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc                                                                  >  1:1666397/2‑69 (MQ=255)
     acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc                                                                  <  1:159881/68‑2 (MQ=255)
     acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg                                                               >  1:1962048/1‑71 (MQ=255)
          tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa                                                                >  1:3565001/1‑65 (MQ=255)
          tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa                                                                >  1:3464236/1‑65 (MQ=255)
          tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                          >  1:1259746/1‑71 (MQ=255)
             tataaCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCa                                                                <  1:2323948/59‑1 (MQ=255)
              aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa                                                       <  1:2709250/70‑1 (MQ=255)
               taaCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTa                                                       <  1:3553339/68‑1 (MQ=255)
                aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt                                                      >  1:2042326/1‑69 (MQ=255)
                 aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCa                                                    <  1:965805/70‑1 (MQ=255)
                 aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg                                                   >  1:807224/1‑71 (MQ=255)
                  cgcgGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGgacgc                                                                  <  1:281607/55‑2 (MQ=255)
                  cGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                            <  1:2240643/61‑1 (MQ=255)
                  cGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg                                                  <  1:2041313/71‑1 (MQ=255)
                     cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt                                                >  1:1650743/1‑70 (MQ=255)
                      ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt                                                      <  1:3104310/63‑1 (MQ=255)
                      ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt                                                      <  1:2732990/63‑1 (MQ=255)
                        ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                             >  1:1316801/1‑70 (MQ=255)
                         tACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCatcc                                           <  1:3462352/71‑4 (MQ=255)
                         tACACCAACGTTCACGAGCTTATCCCATGGCGTACGCTCTCTGGTCGTCa                                                                >  1:655461/1‑50 (MQ=255)
                         tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                          >  1:3221082/1‑56 (MQ=255)
                         tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                          >  1:2049253/1‑56 (MQ=255)
                          acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCa                                           <  1:3838051/70‑1 (MQ=255)
                            acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                            <  1:7733/67‑1 (MQ=255)
                            acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                        <  1:3906863/71‑1 (MQ=255)
                              cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgt                                                                     >  1:1363833/1‑40 (MQ=255)
                              cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                      >  1:107192/1‑71 (MQ=255)
                              cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                      >  1:1683362/1‑71 (MQ=255)
                                  gTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGc                                   >  1:2720589/1‑70 (MQ=255)
                                  gTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCg                                  >  1:3904524/1‑71 (MQ=255)
                                    tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                 >  1:1241453/1‑70 (MQ=255)
                                    tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                 >  1:2369895/1‑70 (MQ=255)
                                     cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                          >  1:2554934/1‑60 (MQ=255)
                                       cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                             <  1:2117088/71‑1 (MQ=255)
                                       cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                             <  1:202207/71‑1 (MQ=255)
                                       cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                             <  1:269066/71‑1 (MQ=255)
                                        gAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                            <  1:205397/71‑1 (MQ=255)
                                         aGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCa                                                             <  1:2546258/37‑1 (MQ=255)
                                           cTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                            <  1:906536/68‑1 (MQ=255)
                                             gATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCg                          <  1:519447/68‑1 (MQ=255)
                                              aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                        <  1:2906606/53‑1 (MQ=255)
                                              aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                        <  1:1306503/69‑1 (MQ=255)
                                              aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                      <  1:3213661/71‑1 (MQ=255)
                                               tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                      >  1:1478975/1‑54 (MQ=255)
                                               tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                      >  1:1831850/1‑54 (MQ=255)
                                               tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                      >  1:3933976/1‑70 (MQ=255)
                                                 ccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                   >  1:3103255/1‑71 (MQ=255)
                                                  cATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc                  >  1:3604711/1‑71 (MQ=255)
                                                   aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGcc                 >  1:1294340/1‑71 (MQ=255)
                                                    tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                            <  1:3759842/43‑1 (MQ=255)
                                                    tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                            <  1:631000/43‑1 (MQ=255)
                                                    tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCct                >  1:649083/1‑71 (MQ=255)
                                                    tGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                <  1:3139982/71‑1 (MQ=255)
                                                      gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa                      <  1:3624194/63‑1 (MQ=255)
                                                      gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa                      <  1:598416/63‑1 (MQ=255)
                                                      gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgc              <  1:1219791/71‑1 (MQ=255)
                                                       cGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctg               <  1:2218364/69‑1 (MQ=255)
                                                        gTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgctg            <  1:2237273/71‑1 (MQ=255)
                                                          aCGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggc          >  1:2513776/1‑70 (MQ=255)
                                                           cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                        >  1:1391373/1‑56 (MQ=255)
                                                           cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt         <  1:1766230/71‑1 (MQ=255)
                                                           cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGt          <  1:1069052/70‑1 (MQ=255)
                                                            gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct         >  1:1339550/1‑68 (MQ=255)
                                                            gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct         >  1:921693/1‑68 (MQ=255)
                                                            gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt        <  1:1594662/71‑1 (MQ=255)
                                                            gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt         >  1:3119623/1‑70 (MQ=255)
                                                             ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                            >  1:1459784/1‑50 (MQ=255)
                                                             ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                            >  1:602307/1‑50 (MQ=255)
                                                             ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                            >  1:1865375/1‑50 (MQ=255)
                                                             ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa       <  1:211357/71‑1 (MQ=255)
                                                                 ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa                    >  1:2364691/1‑54 (MQ=255)
                                                                 ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGt   >  1:372095/1‑71 (MQ=255)
                                                                  tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc  >  1:1663510/1‑71 (MQ=255)
                                                                     tcgtcAGCAACTGTATCAGGATCACCAGTGGATGc                                   <  1:2082184/35‑1 (MQ=255)
                                                                     |                                                                   
TGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTC  >  minE/834596‑834732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: