Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,674 A→G 16.4% Q1044Q (CAA→CAG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6740AG16.4% 163.8 / 25.0 73Q1044Q (CAA→CAGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (32/29);  new base G (4/8);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

TCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACA  >  minE/834606‑834744
                                                                    |                                                                      
tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                                      >  1:1259746/1‑71 (MQ=255)
    aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa                                                                   <  1:2709250/70‑1 (MQ=255)
     taaCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTa                                                                   <  1:3553339/68‑1 (MQ=255)
      aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt                                                                  >  1:2042326/1‑69 (MQ=255)
       aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCa                                                                <  1:965805/70‑1 (MQ=255)
       aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg                                                               >  1:807224/1‑71 (MQ=255)
        cGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                                        <  1:2240643/61‑1 (MQ=255)
        cGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg                                                              <  1:2041313/71‑1 (MQ=255)
           cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGAt                                                            >  1:1650743/1‑70 (MQ=255)
            ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt                                                                  <  1:3104310/63‑1 (MQ=255)
            ggTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt                                                                  <  1:2732990/63‑1 (MQ=255)
              ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                                         >  1:1316801/1‑70 (MQ=255)
               tACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCatcc                                                       <  1:3462352/71‑4 (MQ=255)
               tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                                      >  1:3221082/1‑56 (MQ=255)
               tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                                      >  1:2049253/1‑56 (MQ=255)
                acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCa                                                       <  1:3838051/70‑1 (MQ=255)
                  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                                        <  1:7733/67‑1 (MQ=255)
                  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                    <  1:3906863/71‑1 (MQ=255)
                    cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                  >  1:107192/1‑71 (MQ=255)
                    cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                  >  1:1683362/1‑71 (MQ=255)
                        gTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGc                                               >  1:2720589/1‑70 (MQ=255)
                        gTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCg                                              >  1:3904524/1‑71 (MQ=255)
                          tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                             >  1:2369895/1‑70 (MQ=255)
                          tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                             >  1:1241453/1‑70 (MQ=255)
                           cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                                      >  1:2554934/1‑60 (MQ=255)
                             cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                                         <  1:2117088/71‑1 (MQ=255)
                             cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                                         <  1:202207/71‑1 (MQ=255)
                             cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                                         <  1:269066/71‑1 (MQ=255)
                              gAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                                        <  1:205397/71‑1 (MQ=255)
                                 cTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                                        <  1:906536/68‑1 (MQ=255)
                                   gATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCg                                      <  1:519447/68‑1 (MQ=255)
                                    aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                    <  1:2906606/53‑1 (MQ=255)
                                    aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                    <  1:1306503/69‑1 (MQ=255)
                                    aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                  <  1:3213661/71‑1 (MQ=255)
                                     tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                  >  1:1831850/1‑54 (MQ=255)
                                     tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                  >  1:1478975/1‑54 (MQ=255)
                                     tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                  >  1:3933976/1‑70 (MQ=255)
                                       ccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                               >  1:3103255/1‑71 (MQ=255)
                                        cATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc                              >  1:3604711/1‑71 (MQ=255)
                                         aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGcc                             >  1:1294340/1‑71 (MQ=255)
                                          tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                                        <  1:3759842/43‑1 (MQ=255)
                                          tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                                        <  1:631000/43‑1 (MQ=255)
                                          tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCct                            >  1:649083/1‑71 (MQ=255)
                                          tGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                            <  1:3139982/71‑1 (MQ=255)
                                            gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa                                  <  1:3624194/63‑1 (MQ=255)
                                            gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa                                  <  1:598416/63‑1 (MQ=255)
                                            gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgc                          <  1:1219791/71‑1 (MQ=255)
                                             cGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctg                           <  1:2218364/69‑1 (MQ=255)
                                              gTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgctg                        <  1:2237273/71‑1 (MQ=255)
                                                aCGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggc                      >  1:2513776/1‑70 (MQ=255)
                                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                    >  1:1391373/1‑56 (MQ=255)
                                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                     <  1:1766230/71‑1 (MQ=255)
                                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGt                      <  1:1069052/70‑1 (MQ=255)
                                                  gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct                     >  1:921693/1‑68 (MQ=255)
                                                  gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct                     >  1:1339550/1‑68 (MQ=255)
                                                  gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt                    <  1:1594662/71‑1 (MQ=255)
                                                  gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                     >  1:3119623/1‑70 (MQ=255)
                                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                                        >  1:1865375/1‑50 (MQ=255)
                                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                                        >  1:1459784/1‑50 (MQ=255)
                                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                                        >  1:602307/1‑50 (MQ=255)
                                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                   <  1:211357/71‑1 (MQ=255)
                                                       ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa                                >  1:2364691/1‑54 (MQ=255)
                                                       ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGt               >  1:372095/1‑71 (MQ=255)
                                                        tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc              >  1:1663510/1‑71 (MQ=255)
                                                           tcgtcAGCAACTGTATCAGGATCACCAGTGGATGc                                               <  1:2082184/35‑1 (MQ=255)
                                                             gtcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgccg         <  1:3034171/71‑1 (MQ=255)
                                                             gtcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgccg         <  1:432688/71‑1 (MQ=255)
                                                                agcagcTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                            <  1:463309/49‑1 (MQ=255)
                                                                aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc          >  1:3103029/1‑67 (MQ=255)
                                                                aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc          >  1:1179273/1‑67 (MQ=255)
                                                                 gCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                            <  1:2479820/48‑1 (MQ=255)
                                                                 gCAACTGTATCAGGATCACCAGTGGATTCGTGATTTCGGTGAAAGCCTGCTGGTTTATCg                <  1:2242873/60‑1 (MQ=255)
                                                                 gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                               >  1:3530863/1‑45 (MQ=255)
                                                                 gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCg     <  1:1606177/71‑1 (MQ=255)
                                                                  cAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCGTGCTGGTTTa                   >  1:3890062/1‑56 (MQ=255)
                                                                    aCTGTATCAGGATCACCAGTGGATGGGTGATTTCGGTGAAAGc                              >  1:2328104/1‑43 (MQ=255)
                                                                    aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGaca  >  1:817567/1‑71 (MQ=255)
                                                                    aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGa    <  1:3585401/69‑1 (MQ=255)
                                                                    |                                                                      
TCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACA  >  minE/834606‑834744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: