Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,691 A→C 18.6% Q1050P (CAG→CCG)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6910AC18.6% 143.0 / 44.1 86Q1050P (CAG→CCG) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (36/34);  new base C (8/8);  total (44/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

ACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGA  >  minE/834622‑834756
                                                                     |                                                                 
acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCa                                                                   <  1:3838051/70‑1 (MQ=255)
  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                                <  1:3906863/71‑1 (MQ=255)
    cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                              >  1:107192/1‑71 (MQ=255)
    cAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                              >  1:1683362/1‑71 (MQ=255)
        gTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGc                                                           >  1:2720589/1‑70 (MQ=255)
        gTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCg                                                          >  1:3904524/1‑71 (MQ=255)
          tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                                         >  1:1241453/1‑70 (MQ=255)
          tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                                         >  1:2369895/1‑70 (MQ=255)
           cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                                                  >  1:2554934/1‑60 (MQ=255)
             cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                                                     <  1:269066/71‑1 (MQ=255)
             cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                                                     <  1:2117088/71‑1 (MQ=255)
             cGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                                                     <  1:202207/71‑1 (MQ=255)
              gAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                                                    <  1:205397/71‑1 (MQ=255)
                 cTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                                                    <  1:906536/68‑1 (MQ=255)
                   gATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCg                                                  <  1:519447/68‑1 (MQ=255)
                    aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                                <  1:2906606/53‑1 (MQ=255)
                    aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                                <  1:1306503/69‑1 (MQ=255)
                    aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                              <  1:3213661/71‑1 (MQ=255)
                     tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                              >  1:1478975/1‑54 (MQ=255)
                     tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                              >  1:1831850/1‑54 (MQ=255)
                     tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                              >  1:3933976/1‑70 (MQ=255)
                       ccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                                           >  1:3103255/1‑71 (MQ=255)
                        cATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc                                          >  1:3604711/1‑71 (MQ=255)
                         aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGcc                                         >  1:1294340/1‑71 (MQ=255)
                          tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCct                                        >  1:649083/1‑71 (MQ=255)
                          tGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                                        <  1:3139982/71‑1 (MQ=255)
                            gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa                                              <  1:3624194/63‑1 (MQ=255)
                            gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa                                              <  1:598416/63‑1 (MQ=255)
                            gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgc                                      <  1:1219791/71‑1 (MQ=255)
                             cGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctg                                       <  1:2218364/69‑1 (MQ=255)
                              gTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgctg                                    <  1:2237273/71‑1 (MQ=255)
                                aCGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggc                                  >  1:2513776/1‑70 (MQ=255)
                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                                >  1:1391373/1‑56 (MQ=255)
                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                                 <  1:1766230/71‑1 (MQ=255)
                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGt                                  <  1:1069052/70‑1 (MQ=255)
                                  gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct                                 >  1:1339550/1‑68 (MQ=255)
                                  gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct                                 >  1:921693/1‑68 (MQ=255)
                                  gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt                                <  1:1594662/71‑1 (MQ=255)
                                  gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                                 >  1:3119623/1‑70 (MQ=255)
                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                                                    >  1:602307/1‑50 (MQ=255)
                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                                                    >  1:1459784/1‑50 (MQ=255)
                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAttt                                                    >  1:1865375/1‑50 (MQ=255)
                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                               <  1:211357/71‑1 (MQ=255)
                                       ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa                                            >  1:2364691/1‑54 (MQ=255)
                                       ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGt                           >  1:372095/1‑71 (MQ=255)
                                        tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc                          >  1:1663510/1‑71 (MQ=255)
                                           tcgtcAGCAACTGTATCAGGATCACCAGTGGATGc                                                           <  1:2082184/35‑1 (MQ=255)
                                             gtcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgccg                     <  1:3034171/71‑1 (MQ=255)
                                             gtcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgccg                     <  1:432688/71‑1 (MQ=255)
                                                agcagcTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                                        <  1:463309/49‑1 (MQ=255)
                                                aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                      >  1:1179273/1‑67 (MQ=255)
                                                aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                      >  1:3103029/1‑67 (MQ=255)
                                                 gCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                                        <  1:2479820/48‑1 (MQ=255)
                                                 gCAACTGTATCAGGATCACCAGTGGATTCGTGATTTCGGTGAAAGCCTGCTGGTTTATCg                            <  1:2242873/60‑1 (MQ=255)
                                                 gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                                           >  1:3530863/1‑45 (MQ=255)
                                                 gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCg                 <  1:1606177/71‑1 (MQ=255)
                                                  cAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCGTGCTGGTTTa                               >  1:3890062/1‑56 (MQ=255)
                                                    aCTGTATCAGGATCACCAGTGGATGGGTGATTTCGGTGAAAGc                                          >  1:2328104/1‑43 (MQ=255)
                                                    aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGaca              >  1:817567/1‑71 (MQ=255)
                                                    aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGa                <  1:3585401/69‑1 (MQ=255)
                                                     cTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGacac             >  1:838356/1‑71 (MQ=255)
                                                      tGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATc                  <  1:3039403/65‑1 (MQ=255)
                                                       gTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACAccc           >  1:719501/1‑71 (MQ=255)
                                                         aTCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCg          >  1:1853893/1‑70 (MQ=255)
                                                          tCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCg          >  1:1281944/1‑69 (MQ=255)
                                                          tCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCGta        <  1:309197/71‑2 (MQ=255)
                                                          tCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCGta        <  1:3874277/71‑2 (MQ=255)
                                                          tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc                          >  1:576257/1‑53 (MQ=255)
                                                          tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc                          >  1:580911/1‑53 (MQ=255)
                                                          tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGtt        <  1:3686967/71‑1 (MQ=255)
                                                            aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATc                             >  1:44742/1‑48 (MQ=255)
                                                            aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTc       <  1:984172/70‑1 (MQ=255)
                                                            aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTc       <  1:426008/70‑1 (MQ=255)
                                                             ggATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCGtag       >  1:2337017/1‑67 (MQ=255)
                                                             ggATCAACCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCg                 >  1:192/1‑59 (MQ=255)
                                                              gATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCgcc                          >  1:3401231/1‑47 (MQ=255)
                                                              gATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGt    <  1:2205433/71‑1 (MQ=255)
                                                              gATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGt    <  1:2155285/71‑1 (MQ=255)
                                                                tCACCAGTGGATGCGTGATTTCGGTGAAAGCctgctg                                    <  1:1399864/37‑1 (MQ=255)
                                                                tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccg                        <  1:3398889/49‑1 (MQ=255)
                                                                tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccg                        <  1:1893049/49‑1 (MQ=255)
                                                                tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGa  <  1:3705689/71‑1 (MQ=255)
                                                                 cACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGttt                                <  1:2154747/40‑1 (MQ=255)
                                                                  aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                      >  1:3233194/1‑49 (MQ=255)
                                                                  aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                      >  1:2888313/1‑49 (MQ=255)
                                                                  aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                      >  1:2883648/1‑49 (MQ=255)
                                                                   ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGac               <  1:1158622/55‑1 (MQ=255)
                                                                     aGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGacac             >  1:1098755/1‑55 (MQ=255)
                                                                     |                                                                 
ACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGA  >  minE/834622‑834756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: