Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,707 C→T 19.7% F1055F (TTC→TTT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,7070CT19.7% 172.2 / 34.7 81F1055F (TTC→TTTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (30/35);  new base T (8/8);  total (38/43)
Fisher's exact test for biased strand distribution p-value = 7.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

GATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAA  >  minE/834641‑834776
                                                                  |                                                                     
gATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCg                                                                      <  1:519447/68‑1 (MQ=255)
 aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                                                    <  1:1306503/69‑1 (MQ=255)
 aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                                                  <  1:3213661/71‑1 (MQ=255)
  tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                                                  >  1:3933976/1‑70 (MQ=255)
    ccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                                                               >  1:3103255/1‑71 (MQ=255)
     cATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc                                                              >  1:3604711/1‑71 (MQ=255)
      aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGcc                                                             >  1:1294340/1‑71 (MQ=255)
       tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCct                                                            >  1:649083/1‑71 (MQ=255)
       tGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                                                            <  1:3139982/71‑1 (MQ=255)
         gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa                                                                  <  1:598416/63‑1 (MQ=255)
         gcgcACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGa                                                                  <  1:3624194/63‑1 (MQ=255)
         gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgc                                                          <  1:1219791/71‑1 (MQ=255)
          cGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctg                                                           <  1:2218364/69‑1 (MQ=255)
           gTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgctg                                                        <  1:2237273/71‑1 (MQ=255)
             aCGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggc                                                      >  1:2513776/1‑70 (MQ=255)
              cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGt                                                                    >  1:1391373/1‑56 (MQ=255)
              cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                                                     <  1:1766230/71‑1 (MQ=255)
              cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGt                                                      <  1:1069052/70‑1 (MQ=255)
               gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct                                                     >  1:1339550/1‑68 (MQ=255)
               gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct                                                     >  1:921693/1‑68 (MQ=255)
               gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt                                                    <  1:1594662/71‑1 (MQ=255)
               gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                                                     >  1:3119623/1‑70 (MQ=255)
                ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                                                   <  1:211357/71‑1 (MQ=255)
                    ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa                                                                >  1:2364691/1‑54 (MQ=255)
                    ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGt                                               >  1:372095/1‑71 (MQ=255)
                     tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc                                              >  1:1663510/1‑71 (MQ=255)
                          gtcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgccg                                         <  1:3034171/71‑1 (MQ=255)
                          gtcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgccg                                         <  1:432688/71‑1 (MQ=255)
                             agcagcTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                                                            <  1:463309/49‑1 (MQ=255)
                             aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                                          >  1:1179273/1‑67 (MQ=255)
                             aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                                          >  1:3103029/1‑67 (MQ=255)
                              gCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCct                                                            <  1:2479820/48‑1 (MQ=255)
                              gCAACTGTATCAGGATCACCAGTGGATTCGTGATTTCGGTGAAAGCCTGCTGGTTTATCg                                                <  1:2242873/60‑1 (MQ=255)
                              gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                                                               >  1:3530863/1‑45 (MQ=255)
                              gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCg                                     <  1:1606177/71‑1 (MQ=255)
                               cAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCGTGCTGGTTTa                                                   >  1:3890062/1‑56 (MQ=255)
                                 aCTGTATCAGGATCACCAGTGGATGGGTGATTTCGGTGAAAGc                                                              >  1:2328104/1‑43 (MQ=255)
                                 aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGaca                                  >  1:817567/1‑71 (MQ=255)
                                 aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGa                                    <  1:3585401/69‑1 (MQ=255)
                                  cTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGacac                                 >  1:838356/1‑71 (MQ=255)
                                   tGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATc                                      <  1:3039403/65‑1 (MQ=255)
                                    gTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACAccc                               >  1:719501/1‑71 (MQ=255)
                                      aTCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCg                              >  1:1853893/1‑70 (MQ=255)
                                       tCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCg                              >  1:1281944/1‑69 (MQ=255)
                                       tCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCGta                            <  1:309197/71‑2 (MQ=255)
                                       tCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCGta                            <  1:3874277/71‑2 (MQ=255)
                                       tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc                                              >  1:580911/1‑53 (MQ=255)
                                       tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc                                              >  1:576257/1‑53 (MQ=255)
                                       tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGtt                            <  1:3686967/71‑1 (MQ=255)
                                         aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATc                                                 >  1:44742/1‑48 (MQ=255)
                                         aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTc                           <  1:426008/70‑1 (MQ=255)
                                         aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTc                           <  1:984172/70‑1 (MQ=255)
                                          ggATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCGtag                           >  1:2337017/1‑67 (MQ=255)
                                          ggATCAACCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCg                                     >  1:192/1‑59 (MQ=255)
                                           gATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCgcc                                              >  1:3401231/1‑47 (MQ=255)
                                           gATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGt                        <  1:2155285/71‑1 (MQ=255)
                                           gATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGt                        <  1:2205433/71‑1 (MQ=255)
                                             tCACCAGTGGATGCGTGATTTCGGTGAAAGCctgctg                                                        <  1:1399864/37‑1 (MQ=255)
                                             tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccg                                            <  1:3398889/49‑1 (MQ=255)
                                             tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccg                                            <  1:1893049/49‑1 (MQ=255)
                                             tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGa                      <  1:3705689/71‑1 (MQ=255)
                                              cACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGttt                                                    <  1:2154747/40‑1 (MQ=255)
                                               aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                                          >  1:2888313/1‑49 (MQ=255)
                                               aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                                          >  1:2883648/1‑49 (MQ=255)
                                               aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                                          >  1:3233194/1‑49 (MQ=255)
                                                ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGac                                   <  1:1158622/55‑1 (MQ=255)
                                                  aGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGacac                                 >  1:1098755/1‑55 (MQ=255)
                                                    tGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGa                                        >  1:3342206/1‑46 (MQ=255)
                                                    tGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGa                                        >  1:1193428/1‑46 (MQ=255)
                                                       aTGCGTGATTTCGGTGAAAGCGTGCTGGTTTATCGTccgcc                                          <  1:871147/41‑1 (MQ=255)
                                                       aTGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGAt            <  1:3639100/71‑1 (MQ=255)
                                                         gCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgccg                                         <  1:2224679/40‑1 (MQ=255)
                                                         gCGTGATTTCGGTGAAAGCCTGCTGGTGTATCGTccgccg                                         <  1:3564993/40‑1 (MQ=255)
                                                            tGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGaca                                  <  1:3750205/44‑1 (MQ=255)
                                                            tGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGa                                    >  1:2025409/1‑42 (MQ=255)
                                                            tGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGa                                    >  1:1284508/1‑42 (MQ=255)
                                                            tGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGtt                            <  1:2024037/50‑1 (MQ=255)
                                                            tGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGtt                            <  1:852786/50‑1 (MQ=255)
                                                              aTTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAg     <  1:2938012/71‑1 (MQ=255)
                                                                 tCGGTGAAATCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGaa   <  1:1241974/70‑1 (MQ=255)
                                                                 tCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGaaa  >  1:772528/1‑71 (MQ=255)
                                                                  cGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGaaa  <  1:707921/70‑1 (MQ=255)
                                                                  |                                                                     
GATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAA  >  minE/834641‑834776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: