| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,731 | T→C | 8.3% | R1063R (CGT→CGC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,731 | 0 | T | C | 8.3% | 196.6 / 7.9 | 72 | R1063R (CGT→CGC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (26/40); new base C (4/2); total (30/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
CTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGC > minE/834661‑834801 | ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGt > 1:372095/1‑71 (MQ=255) tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc > 1:1663510/1‑71 (MQ=255) gtcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgccg < 1:432688/71‑1 (MQ=255) gtcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgccg < 1:3034171/71‑1 (MQ=255) aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc > 1:1179273/1‑67 (MQ=255) aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc > 1:3103029/1‑67 (MQ=255) gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCg < 1:1606177/71‑1 (MQ=255) aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGaca > 1:817567/1‑71 (MQ=255) aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGa < 1:3585401/69‑1 (MQ=255) cTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGacac > 1:838356/1‑71 (MQ=255) tGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATc < 1:3039403/65‑1 (MQ=255) gTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACAccc > 1:719501/1‑71 (MQ=255) aTCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCg > 1:1853893/1‑70 (MQ=255) tCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCg > 1:1281944/1‑69 (MQ=255) tCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCGta < 1:309197/71‑2 (MQ=255) tCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCGta < 1:3874277/71‑2 (MQ=255) tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc > 1:576257/1‑53 (MQ=255) tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc > 1:580911/1‑53 (MQ=255) tCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGtt < 1:3686967/71‑1 (MQ=255) aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTc < 1:984172/70‑1 (MQ=255) aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTc < 1:426008/70‑1 (MQ=255) ggATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCGtag > 1:2337017/1‑67 (MQ=255) ggATCAACCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCg > 1:192/1‑59 (MQ=255) gATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGt < 1:2205433/71‑1 (MQ=255) gATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGt < 1:2155285/71‑1 (MQ=255) tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccg < 1:3398889/49‑1 (MQ=255) tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccg < 1:1893049/49‑1 (MQ=255) tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGa < 1:3705689/71‑1 (MQ=255) aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc > 1:3233194/1‑49 (MQ=255) aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc > 1:2883648/1‑49 (MQ=255) aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc > 1:2888313/1‑49 (MQ=255) ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGac < 1:1158622/55‑1 (MQ=255) aGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGacac > 1:1098755/1‑55 (MQ=255) tGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGa > 1:3342206/1‑46 (MQ=255) tGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGa > 1:1193428/1‑46 (MQ=255) aTGCGTGATTTCGGTGAAAGCGTGCTGGTTTATCGTccgcc < 1:871147/41‑1 (MQ=255) aTGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGAt < 1:3639100/71‑1 (MQ=255) gCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgccg < 1:2224679/40‑1 (MQ=255) gCGTGATTTCGGTGAAAGCCTGCTGGTGTATCGTccgccg < 1:3564993/40‑1 (MQ=255) tGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGaca < 1:3750205/44‑1 (MQ=255) tGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGa > 1:2025409/1‑42 (MQ=255) tGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGa > 1:1284508/1‑42 (MQ=255) tGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGtt < 1:852786/50‑1 (MQ=255) tGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGtt < 1:2024037/50‑1 (MQ=255) aTTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAg < 1:2938012/71‑1 (MQ=255) tCGGTGAAATCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGaa < 1:1241974/70‑1 (MQ=255) tCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGaaa > 1:772528/1‑71 (MQ=255) cGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGaaa < 1:707921/70‑1 (MQ=255) gTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTc < 1:55652/43‑1 (MQ=255) tGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGa < 1:1183517/47‑1 (MQ=255) tGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGa < 1:2454449/47‑1 (MQ=255) gAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGaa > 1:3474268/1‑47 (MQ=255) gAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAaagaa > 1:2420037/1‑51 (MQ=255) aaaGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCgg > 1:1152171/1‑42 (MQ=255) aaaGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGt < 1:948919/52‑1 (MQ=255) aaGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACg > 1:3408497/1‑71 (MQ=255) aGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGt < 1:1688354/50‑1 (MQ=255) gCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGt < 1:2708890/49‑1 (MQ=255) ccTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTg > 1:3106249/1‑49 (MQ=255) ccTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTg > 1:633183/1‑49 (MQ=255) ctgGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAAcc < 1:2243051/70‑1 (MQ=255) ctgGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAAc > 1:2115524/1‑69 (MQ=255) tATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGaaa < 1:1290459/70‑1 (MQ=255) tATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGaaa < 1:1709648/70‑1 (MQ=255) tATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGaaa < 1:1714168/70‑1 (MQ=255) tATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGaaa < 1:695058/70‑1 (MQ=255) tATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGaaa < 1:382810/70‑1 (MQ=255) aTCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATc < 1:2097804/52‑1 (MQ=255) tCGTCCGCCCATCGACACCCGTTCGGTGAAAGAAGTGAt > 1:2184057/1‑39 (MQ=255) gTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGAt < 1:1420284/37‑1 (MQ=255) gTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACgg > 1:2147811/1‑55 (MQ=255) gTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAgcg < 1:878384/71‑1 (MQ=255) gTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAgc < 1:2004777/70‑1 (MQ=255) tCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAgcgc < 1:2947793/71‑1 (MQ=255) | CTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGC > minE/834661‑834801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |