| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,827 | G→A | 15.6% | K1095K (AAG→AAA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,827 | 0 | G | A | 15.6% | 205.0 / 26.0 | 83 | K1095K (AAG→AAA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (40/30); new base A (5/8); total (45/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
AGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCT > minE/834758‑834897 | agaagTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGt < 1:2276095/71‑1 (MQ=255) aagTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTgg > 1:1056158/1‑71 (MQ=255) aagTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTgg > 1:3140525/1‑71 (MQ=255) agTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTggg > 1:2594176/1‑71 (MQ=255) aGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtc > 1:362233/1‑70 (MQ=255) ggCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtcc < 1:3281567/70‑1 (MQ=255) gCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGt < 1:2903754/60‑1 (MQ=255) gCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGt < 1:608363/60‑1 (MQ=255) cAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactc > 1:2540769/1‑71 (MQ=255) cAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactc > 1:872743/1‑71 (MQ=255) aaTCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCAcc < 1:2703181/71‑1 (MQ=255) tCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTa < 1:3760787/71‑1 (MQ=255) aaCGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAg < 1:1480153/71‑1 (MQ=255) aCGGCTTCCCGGAAAAAGCACTTAACTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAgt < 1:3301603/71‑2 (MQ=255) cGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactcca > 1:1097051/1‑62 (MQ=255) cGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGc > 1:1278335/1‑70 (MQ=255) cAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtcca > 1:2065016/1‑54 (MQ=255) cccggAAAAAGCACTTAACTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGaaaa < 1:502034/66‑1 (MQ=255) ccAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTa > 1:2827921/1‑60 (MQ=255) ccAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAAcc > 1:2987851/1‑71 (MQ=255) cAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactc < 1:1826382/53‑1 (MQ=255) cAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCt < 1:3233642/58‑1 (MQ=255) aGGATAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCg < 1:3719383/63‑1 (MQ=255) aGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctg < 1:1647254/71‑1 (MQ=255) ggAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTgg > 1:53685/1‑40 (MQ=255) ggAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgc > 1:3222517/1‑71 (MQ=255) ggAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctg > 1:1773966/1‑70 (MQ=255) gAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTAc < 1:635203/57‑1 (MQ=255) gAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgct < 1:3359566/71‑1 (MQ=255) aaaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactccac > 1:493546/1‑52 (MQ=255) aaaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgct > 1:1239461/1‑70 (MQ=255) aaaaaGCACTTAACTTCCTGACGCCGCACCAGAAATGGGGCAtt < 1:3791236/44‑2 (MQ=25) aaaaaGCACTTAACTTCCTGACGCCGCACCAGAAATGGGGCAtt < 1:2028140/44‑2 (MQ=25) aaaaaGCACTTAACTTCCTGACGCCGCACCAGAAATGGGGCAtt < 1:1684770/44‑2 (MQ=25) aaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtcca > 1:1865719/1‑45 (MQ=255) aaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga > 1:3368332/1‑71 (MQ=255) aGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgc > 1:471461/1‑71 (MQ=255) cttaaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAc > 1:92381/4‑71 (MQ=255) cTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACct > 1:2632749/1‑59 (MQ=255) ttaaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCtgctaatgctga < 1:3420312/67‑1 (MQ=255) tCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactcca > 1:2996528/1‑42 (MQ=255) tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc > 1:1547893/1‑53 (MQ=255) tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc > 1:3270975/1‑53 (MQ=255) tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc > 1:3593428/1‑53 (MQ=255) taaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTa > 1:2186925/2‑45 (MQ=25) taaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTa > 1:2115153/2‑45 (MQ=25) accTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt < 1:1784542/68‑1 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTAtccactc < 1:3318223/38‑1 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCttctgatgct > 1:1950809/1‑65 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgct < 1:3065896/65‑1 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgct > 1:1164855/1‑65 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga < 1:3809407/61‑1 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga < 1:3378406/61‑1 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga < 1:3414901/61‑1 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt < 1:1815356/71‑1 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt < 1:2880739/71‑1 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt > 1:2004276/1‑70 (MQ=255) aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt < 1:2077079/70‑1 (MQ=255) aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcgct > 1:3011110/1‑68 (MQ=255) aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcgct > 1:1137890/1‑68 (MQ=255) aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcg < 1:3897939/69‑2 (MQ=255) aCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt < 1:3239949/70‑1 (MQ=255) ttCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg > 1:523305/1‑71 (MQ=255) tCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatg > 1:3638768/1‑59 (MQ=255) tCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGAc > 1:2403516/1‑64 (MQ=255) ccTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGt < 1:2850014/70‑1 (MQ=255) cTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTcg < 1:910813/71‑1 (MQ=255) aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGc > 1:3915758/1‑39 (MQ=255) aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGgcgctg < 1:440874/71‑3 (MQ=255) aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg < 1:404062/71‑1 (MQ=255) aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg < 1:2389547/71‑1 (MQ=255) aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg < 1:3023732/71‑1 (MQ=255) ccgcACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGACGCTCTCTCGcggtgg < 1:1357948/71‑1 (MQ=255) gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTa > 1:3388997/1‑58 (MQ=255) gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTa > 1:3895735/1‑58 (MQ=255) gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggt > 1:1028919/1‑70 (MQ=255) aCCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg > 1:3117071/1‑58 (MQ=255) aCCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg > 1:3205978/1‑58 (MQ=255) ccAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccgg < 1:1066846/71‑1 (MQ=255) aGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtg > 1:1695133/1‑71 (MQ=255) gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTcgcg > 1:1823140/1‑59 (MQ=255) gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtgg > 1:256754/1‑71 (MQ=255) gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtgg > 1:850214/1‑71 (MQ=255) gTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCt > 1:1006801/1‑71 (MQ=255) | AGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCT > minE/834758‑834897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |