Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,836 C→T 14.5% I1098I (ATC→ATT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,8360CT14.5% 175.8 / 18.3 69I1098I (ATC→ATTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (32/27);  new base T (5/5);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

AGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGT  >  minE/834767‑834905
                                                                     |                                                                     
aGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtc                                                                       >  1:362233/1‑70 (MQ=255)
 ggCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtcc                                                                      <  1:3281567/70‑1 (MQ=255)
    cAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactc                                                                  >  1:872743/1‑71 (MQ=255)
    cAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactc                                                                  >  1:2540769/1‑71 (MQ=255)
        aaTCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCAcc                                                              <  1:2703181/71‑1 (MQ=255)
          tCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTa                                                            <  1:3760787/71‑1 (MQ=255)
             aaCGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAg                                                         <  1:1480153/71‑1 (MQ=255)
              aCGGCTTCCCGGAAAAAGCACTTAACTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAgt                                                        <  1:3301603/71‑2 (MQ=255)
               cGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactcca                                                                >  1:1097051/1‑62 (MQ=255)
               cGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGc                                                        >  1:1278335/1‑70 (MQ=255)
                  cAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtcca                                                                     >  1:2065016/1‑54 (MQ=255)
                     cccggAAAAAGCACTTAACTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGaaaa                                                   <  1:502034/66‑1 (MQ=255)
                     ccAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTa                                                            >  1:2827921/1‑60 (MQ=255)
                     ccAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAAcc                                                 >  1:2987851/1‑71 (MQ=255)
                      cAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactc                                                                  <  1:1826382/53‑1 (MQ=255)
                      cAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCt                                                             <  1:3233642/58‑1 (MQ=255)
                       aGGATAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCg                                                       <  1:3719383/63‑1 (MQ=255)
                       aGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctg                                               <  1:1647254/71‑1 (MQ=255)
                        ggAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgc                                              >  1:3222517/1‑71 (MQ=255)
                        ggAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctg                                               >  1:1773966/1‑70 (MQ=255)
                         gAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTAc                                                           <  1:635203/57‑1 (MQ=255)
                         gAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgct                                             <  1:3359566/71‑1 (MQ=255)
                          aaaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactccac                                                               >  1:493546/1‑52 (MQ=255)
                          aaaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgct                                             >  1:1239461/1‑70 (MQ=255)
                           aaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtcca                                                                     >  1:1865719/1‑45 (MQ=255)
                           aaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                           >  1:3368332/1‑71 (MQ=255)
                              aGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgc                                        >  1:471461/1‑71 (MQ=255)
                                  cttaaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAc                                    >  1:92381/4‑71 (MQ=255)
                                  cTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACct                                                >  1:2632749/1‑59 (MQ=255)
                                   ttaaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCtgctaatgctga                                     <  1:3420312/67‑1 (MQ=255)
                                   tCAACTTCCTGACGCCGCACCAGAAGTGGGGTAtccactcca                                                                >  1:2996528/1‑42 (MQ=255)
                                   tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc                                                     >  1:3593428/1‑53 (MQ=255)
                                   tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc                                                     >  1:3270975/1‑53 (MQ=255)
                                   tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc                                                     >  1:1547893/1‑53 (MQ=255)
                                    taaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTa                                                            >  1:2115153/2‑45 (MQ=25)
                                    taaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTa                                                            >  1:2186925/2‑45 (MQ=25)
                                     accTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt                                  <  1:1784542/68‑1 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTAtccactc                                                                  <  1:3318223/38‑1 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCttctgatgct                                       >  1:1950809/1‑65 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgct                                       <  1:3065896/65‑1 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgct                                       >  1:1164855/1‑65 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                           <  1:3809407/61‑1 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                           <  1:3378406/61‑1 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                           <  1:3414901/61‑1 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt                                 <  1:2880739/71‑1 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt                                 <  1:1815356/71‑1 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt                                  >  1:2004276/1‑70 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt                                  <  1:2077079/70‑1 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcgct                                 >  1:3011110/1‑68 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcgct                                 >  1:1137890/1‑68 (MQ=255)
                                     aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcg                                   <  1:3897939/69‑2 (MQ=255)
                                      aCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt                                 <  1:3239949/70‑1 (MQ=255)
                                        ttCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg                              >  1:523305/1‑71 (MQ=255)
                                         tCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatg                                         >  1:3638768/1‑59 (MQ=255)
                                         tCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGAc                                    >  1:2403516/1‑64 (MQ=255)
                                          ccTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGt                             <  1:2850014/70‑1 (MQ=255)
                                           cTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTcg                           <  1:910813/71‑1 (MQ=255)
                                              aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGc                                                        >  1:3915758/1‑39 (MQ=255)
                                              aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGgcgctg                        <  1:440874/71‑3 (MQ=255)
                                              aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg                        <  1:404062/71‑1 (MQ=255)
                                              aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg                        <  1:3023732/71‑1 (MQ=255)
                                              aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg                        <  1:2389547/71‑1 (MQ=255)
                                                 ccgcACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGACGCTCTCTCGcggtgg                     <  1:1357948/71‑1 (MQ=255)
                                                   gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTa                                >  1:3388997/1‑58 (MQ=255)
                                                   gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTa                                >  1:3895735/1‑58 (MQ=255)
                                                   gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggt                    >  1:1028919/1‑70 (MQ=255)
                                                     aCCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg                              >  1:3117071/1‑58 (MQ=255)
                                                     aCCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg                              >  1:3205978/1‑58 (MQ=255)
                                                      ccAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccgg                <  1:1066846/71‑1 (MQ=255)
                                                        aGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtg              >  1:1695133/1‑71 (MQ=255)
                                                         gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTcgcg                         >  1:1823140/1‑59 (MQ=255)
                                                         gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtgg             >  1:850214/1‑71 (MQ=255)
                                                         gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtgg             >  1:256754/1‑71 (MQ=255)
                                                            gTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCt          >  1:1006801/1‑71 (MQ=255)
                                                              ggggTATCCACTCCACCTACAGCGACAACCTGCTTATGCTGACTTTAGGTCGcggt                       <  1:2391963/56‑1 (MQ=255)
                                                               gggTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGt       >  1:290169/1‑71 (MQ=255)
                                                                ggTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGtt      <  1:374674/71‑1 (MQ=255)
                                                                ggTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGGCGCGGTGGTCCGGTGGTCTGGtt      <  1:2154210/71‑1 (MQ=255)
                                                                   aTCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg   >  1:1911405/1‑71 (MQ=255)
                                                                   aTCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg   >  1:1400834/1‑71 (MQ=255)
                                                                    tccactccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg   >  1:3227962/1‑70 (MQ=255)
                                                                    tccactccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGt  >  1:815610/1‑71 (MQ=255)
                                                                     |                                                                     
AGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGT  >  minE/834767‑834905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: