Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,851 C→T 8.7% S1103S (AGC→AGT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,8510CT 8.7% 236.0 / 9.6 80S1103S (AGC→AGTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (46/27);  new base T (3/4);  total (49/31)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

CGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAG  >  minE/834782‑834921
                                                                     |                                                                      
cGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGc                                                                        >  1:1278335/1‑70 (MQ=255)
      cccggAAAAAGCACTTAACTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGaaaa                                                                   <  1:502034/66‑1 (MQ=255)
      ccAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAAcc                                                                 >  1:2987851/1‑71 (MQ=255)
        aGGATAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCg                                                                       <  1:3719383/63‑1 (MQ=255)
        aGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctg                                                               <  1:1647254/71‑1 (MQ=255)
         ggAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgc                                                              >  1:3222517/1‑71 (MQ=255)
         ggAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctg                                                               >  1:1773966/1‑70 (MQ=255)
          gAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgct                                                             <  1:3359566/71‑1 (MQ=255)
           aaaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgct                                                             >  1:1239461/1‑70 (MQ=255)
            aaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                                           >  1:3368332/1‑71 (MQ=255)
               aGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgc                                                        >  1:471461/1‑71 (MQ=255)
                   cttaaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAc                                                    >  1:92381/4‑71 (MQ=255)
                   cTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACct                                                                >  1:2632749/1‑59 (MQ=255)
                    ttaaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCtgctaatgctga                                                     <  1:3420312/67‑1 (MQ=255)
                    tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc                                                                     >  1:1547893/1‑53 (MQ=255)
                    tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc                                                                     >  1:3593428/1‑53 (MQ=255)
                    tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc                                                                     >  1:3270975/1‑53 (MQ=255)
                      accTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt                                                  <  1:1784542/68‑1 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCttctgatgct                                                       >  1:1950809/1‑65 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgct                                                       <  1:3065896/65‑1 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgct                                                       >  1:1164855/1‑65 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                                           <  1:3378406/61‑1 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                                           <  1:3809407/61‑1 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                                           <  1:3414901/61‑1 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt                                                 <  1:2880739/71‑1 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt                                                 <  1:1815356/71‑1 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt                                                  >  1:2004276/1‑70 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt                                                  <  1:2077079/70‑1 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcgct                                                 >  1:3011110/1‑68 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcgct                                                 >  1:1137890/1‑68 (MQ=255)
                      aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcg                                                   <  1:3897939/69‑2 (MQ=255)
                       aCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt                                                 <  1:3239949/70‑1 (MQ=255)
                         ttCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg                                              >  1:523305/1‑71 (MQ=255)
                          tCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatg                                                         >  1:3638768/1‑59 (MQ=255)
                          tCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGAc                                                    >  1:2403516/1‑64 (MQ=255)
                           ccTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGt                                             <  1:2850014/70‑1 (MQ=255)
                            cTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTcg                                           <  1:910813/71‑1 (MQ=255)
                               aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGc                                                                        >  1:3915758/1‑39 (MQ=255)
                               aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGgcgctg                                        <  1:440874/71‑3 (MQ=255)
                               aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg                                        <  1:2389547/71‑1 (MQ=255)
                               aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg                                        <  1:404062/71‑1 (MQ=255)
                               aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg                                        <  1:3023732/71‑1 (MQ=255)
                                  ccgcACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGACGCTCTCTCGcggtgg                                     <  1:1357948/71‑1 (MQ=255)
                                    gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTa                                                >  1:3388997/1‑58 (MQ=255)
                                    gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTa                                                >  1:3895735/1‑58 (MQ=255)
                                    gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggt                                    >  1:1028919/1‑70 (MQ=255)
                                      aCCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg                                              >  1:3117071/1‑58 (MQ=255)
                                      aCCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg                                              >  1:3205978/1‑58 (MQ=255)
                                       ccAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccgg                                <  1:1066846/71‑1 (MQ=255)
                                         aGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtg                              >  1:1695133/1‑71 (MQ=255)
                                          gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTcgcg                                         >  1:1823140/1‑59 (MQ=255)
                                          gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtgg                             >  1:850214/1‑71 (MQ=255)
                                          gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtgg                             >  1:256754/1‑71 (MQ=255)
                                             gTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCt                          >  1:1006801/1‑71 (MQ=255)
                                               ggggTATCCACTCCACCTACAGCGACAACCTGCTTATGCTGACTTTAGGTCGcggt                                       <  1:2391963/56‑1 (MQ=255)
                                                gggTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGt                       >  1:290169/1‑71 (MQ=255)
                                                 ggTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGtt                      <  1:374674/71‑1 (MQ=255)
                                                 ggTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGGCGCGGTGGTCCGGTGGTCTGGtt                      <  1:2154210/71‑1 (MQ=255)
                                                    aTCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg                   >  1:1400834/1‑71 (MQ=255)
                                                    aTCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg                   >  1:1911405/1‑71 (MQ=255)
                                                     tccactccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg                   >  1:3227962/1‑70 (MQ=255)
                                                     tccactccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGt                  >  1:815610/1‑71 (MQ=255)
                                                       cactccacCTACAGCGACAACCTGCTGATGCTGACtt                                                  <  1:2898683/37‑1 (MQ=255)
                                                       cactccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTg                 >  1:1199259/1‑70 (MQ=255)
                                                       cactccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGa                <  1:134539/71‑1 (MQ=255)
                                                       cactccacCTACAGCGACAACCTGCTGATGATGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTg                 <  1:2986588/70‑1 (MQ=255)
                                                        actccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTg                 >  1:1251685/1‑69 (MQ=255)
                                                        actacacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGa                    >  1:3088452/1‑66 (MQ=255)
                                                         ctccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAg              >  1:389281/1‑71 (MQ=255)
                                                           ccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGcc            >  1:2838486/1‑71 (MQ=255)
                                                            cacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGcc            >  1:2405061/1‑70 (MQ=255)
                                                            cacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGcc            <  1:1595924/70‑1 (MQ=255)
                                                            cacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGcc            >  1:2409675/1‑70 (MQ=255)
                                                            cacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGc             >  1:822365/1‑69 (MQ=255)
                                                              ccTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCAGGTGGTCTGGTTGAGTGAAGCCGAt         >  1:1985097/1‑71 (MQ=255)
                                                               cTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATg        >  1:3515933/1‑71 (MQ=255)
                                                                tACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATg        >  1:1537513/1‑70 (MQ=255)
                                                                tACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGc       >  1:1452382/1‑71 (MQ=255)
                                                                 aCAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg                   >  1:1689172/1‑58 (MQ=255)
                                                                 aCAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGc             >  1:1028700/1‑64 (MQ=255)
                                                                  cAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGcc      >  1:3432029/1‑70 (MQ=255)
                                                                  cAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCa     <  1:47024/71‑1 (MQ=255)
                                                                     cGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGc             >  1:938608/1‑60 (MQ=255)
                                                                     cGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCa     >  1:3762694/1‑68 (MQ=255)
                                                                     cGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAg  <  1:1243612/71‑1 (MQ=255)
                                                                     |                                                                      
CGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAG  >  minE/834782‑834921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: