Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,854 C→A 7.3% D1104E (GAC→GAA narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,8540CA 7.3% 269.0 / 3.6 81D1104E (GAC→GAAnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (45/30);  new base A (3/3);  total (48/33)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGAT  >  minE/834788‑834923
                                                                  |                                                                     
cccggAAAAAGCACTTAACTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGaaaa                                                                     <  1:502034/66‑1 (MQ=255)
ccAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAAcc                                                                   >  1:2987851/1‑71 (MQ=255)
  aGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctg                                                                 <  1:1647254/71‑1 (MQ=255)
   ggAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgc                                                                >  1:3222517/1‑71 (MQ=255)
   ggAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctg                                                                 >  1:1773966/1‑70 (MQ=255)
    gAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgct                                                               <  1:3359566/71‑1 (MQ=255)
     aaaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctgct                                                               >  1:1239461/1‑70 (MQ=255)
      aaaaGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                                             >  1:3368332/1‑71 (MQ=255)
         aGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgc                                                          >  1:471461/1‑71 (MQ=255)
             cttaaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAc                                                      >  1:92381/4‑71 (MQ=255)
             cTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACct                                                                  >  1:2632749/1‑59 (MQ=255)
              ttaaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCtgctaatgctga                                                       <  1:3420312/67‑1 (MQ=255)
              tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc                                                                       >  1:3593428/1‑53 (MQ=255)
              tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc                                                                       >  1:1547893/1‑53 (MQ=255)
              tCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGAc                                                                       >  1:3270975/1‑53 (MQ=255)
                accTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt                                                    <  1:1784542/68‑1 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCttctgatgct                                                         >  1:1950809/1‑65 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgct                                                         <  1:3065896/65‑1 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgct                                                         >  1:1164855/1‑65 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                                             <  1:3809407/61‑1 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                                             <  1:3378406/61‑1 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctga                                                             <  1:3414901/61‑1 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt                                                   <  1:2880739/71‑1 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt                                                   <  1:1815356/71‑1 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt                                                    <  1:2077079/70‑1 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACtt                                                    >  1:2004276/1‑70 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcgct                                                   >  1:3011110/1‑68 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcgct                                                   >  1:1137890/1‑68 (MQ=255)
                aaCTTCCTGACGCCGCACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGAcg                                                     <  1:3897939/69‑2 (MQ=255)
                 aCTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttt                                                   <  1:3239949/70‑1 (MQ=255)
                   ttCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg                                                >  1:523305/1‑71 (MQ=255)
                    tCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatg                                                           >  1:3638768/1‑59 (MQ=255)
                    tCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGAc                                                      >  1:2403516/1‑64 (MQ=255)
                     ccTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGt                                               <  1:2850014/70‑1 (MQ=255)
                      cTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTcg                                             <  1:910813/71‑1 (MQ=255)
                         aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGgcgctg                                          <  1:440874/71‑3 (MQ=255)
                         aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg                                          <  1:3023732/71‑1 (MQ=255)
                         aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg                                          <  1:2389547/71‑1 (MQ=255)
                         aCGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcgg                                          <  1:404062/71‑1 (MQ=255)
                            ccgcACCAGAAATGGGGCATTCACTCAACCTACAGTGAAAACCTGCTAATGCTGACGCTCTCTCGcggtgg                                       <  1:1357948/71‑1 (MQ=255)
                              gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTa                                                  >  1:3388997/1‑58 (MQ=255)
                              gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTa                                                  >  1:3895735/1‑58 (MQ=255)
                              gcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggt                                      >  1:1028919/1‑70 (MQ=255)
                                aCCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg                                                >  1:3205978/1‑58 (MQ=255)
                                aCCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAgg                                                >  1:3117071/1‑58 (MQ=255)
                                 ccAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccgg                                  <  1:1066846/71‑1 (MQ=255)
                                   aGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtg                                >  1:1695133/1‑71 (MQ=255)
                                    gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTcgcg                                           >  1:1823140/1‑59 (MQ=255)
                                    gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtgg                               >  1:256754/1‑71 (MQ=255)
                                    gAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtgg                               >  1:850214/1‑71 (MQ=255)
                                       gTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCt                            >  1:1006801/1‑71 (MQ=255)
                                         ggggTATCCACTCCACCTACAGCGACAACCTGCTTATGCTGACTTTAGGTCGcggt                                         <  1:2391963/56‑1 (MQ=255)
                                          gggTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGt                         >  1:290169/1‑71 (MQ=255)
                                           ggTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGtt                        <  1:374674/71‑1 (MQ=255)
                                           ggTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGGCGCGGTGGTCCGGTGGTCTGGtt                        <  1:2154210/71‑1 (MQ=255)
                                              aTCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg                     >  1:1400834/1‑71 (MQ=255)
                                              aTCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg                     >  1:1911405/1‑71 (MQ=255)
                                               tccactccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg                     >  1:3227962/1‑70 (MQ=255)
                                               tccactccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGt                    >  1:815610/1‑71 (MQ=255)
                                                 cactccacCTACAGCGACAACCTGCTGATGCTGACtt                                                    <  1:2898683/37‑1 (MQ=255)
                                                 cactccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTg                   >  1:1199259/1‑70 (MQ=255)
                                                 cactccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGa                  <  1:134539/71‑1 (MQ=255)
                                                 cactccacCTACAGCGACAACCTGCTGATGATGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTg                   <  1:2986588/70‑1 (MQ=255)
                                                  actccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTg                   >  1:1251685/1‑69 (MQ=255)
                                                  actacacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGa                      >  1:3088452/1‑66 (MQ=255)
                                                   ctccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAg                >  1:389281/1‑71 (MQ=255)
                                                     ccacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGcc              >  1:2838486/1‑71 (MQ=255)
                                                      cacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGcc              >  1:2405061/1‑70 (MQ=255)
                                                      cacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGcc              >  1:2409675/1‑70 (MQ=255)
                                                      cacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGcc              <  1:1595924/70‑1 (MQ=255)
                                                      cacCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGc               >  1:822365/1‑69 (MQ=255)
                                                        ccTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCAGGTGGTCTGGTTGAGTGAAGCCGAt           >  1:1985097/1‑71 (MQ=255)
                                                         cTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATg          >  1:3515933/1‑71 (MQ=255)
                                                          tACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATg          >  1:1537513/1‑70 (MQ=255)
                                                          tACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGc         >  1:1452382/1‑71 (MQ=255)
                                                           aCAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAg                     >  1:1689172/1‑58 (MQ=255)
                                                           aCAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGc               >  1:1028700/1‑64 (MQ=255)
                                                            cAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGcc        >  1:3432029/1‑70 (MQ=255)
                                                            cAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCa       <  1:47024/71‑1 (MQ=255)
                                                               cGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGc               >  1:938608/1‑60 (MQ=255)
                                                               cGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCa       >  1:3762694/1‑68 (MQ=255)
                                                               cGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAg    <  1:1243612/71‑1 (MQ=255)
                                                                gACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGc               <  1:1215518/59‑1 (MQ=255)
                                                                gACAACCTGCTGATGCTGACTTTAGGGCGCGGTGGGCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAg    <  1:1056918/70‑1 (MQ=255)
                                                                 aCAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGAt  <  1:1797957/71‑1 (MQ=255)
                                                                  |                                                                     
CCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGAT  >  minE/834788‑834923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: