Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,971 T→C 7.4% G1143G (GGT→GGC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,9710TC 7.4% 219.9 / 7.8 81G1143G (GGT→GGCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (26/49);  new base C (2/4);  total (28/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

GTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGG  >  minE/834904‑835041
                                                                   |                                                                      
gTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAATAGCAACGGTGct                                                                     >  1:64183/1‑71 (MQ=255)
gTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGct                                                                     >  1:1741899/1‑71 (MQ=255)
 tGAAGCCGATGCCAAAGATCTGTGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctc                                                                    <  1:394803/71‑1 (MQ=255)
   aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                                   >  1:506076/1‑70 (MQ=255)
   aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                                   >  1:930899/1‑70 (MQ=255)
   aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                                   <  1:3112857/70‑1 (MQ=255)
   aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctc                                                                    >  1:506077/1‑69 (MQ=255)
    aGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTTGATTGAAGTCTTCAACAGCAACGGTGCTCTGa                                                                 <  1:285491/71‑1 (MQ=255)
    aGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGa                                                                 <  1:2811445/71‑1 (MQ=255)
      ccGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACt                                                               <  1:1281711/71‑1 (MQ=255)
       cGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTg                                                              >  1:3517072/1‑71 (MQ=255)
        gATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTg                                                              >  1:207762/1‑70 (MQ=255)
        gATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGc                                                             <  1:3276774/71‑1 (MQ=255)
         aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGcc                                                            >  1:1762631/1‑71 (MQ=255)
            ccAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCg                                                          <  1:1543994/70‑1 (MQ=255)
              aaaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGc                                                       <  1:2125921/71‑1 (MQ=255)
               aaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCg                                                      >  1:1180998/1‑71 (MQ=255)
                aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg                                                     <  1:2842143/71‑1 (MQ=255)
                aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg                                                     <  1:2988767/71‑1 (MQ=255)
                aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg                                                     >  1:2575661/1‑71 (MQ=255)
                aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg                                                     <  1:2819798/71‑1 (MQ=255)
                aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg                                                     <  1:268631/71‑1 (MQ=255)
                aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg                                                     <  1:2011002/71‑1 (MQ=255)
                aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg                                                     <  1:938874/71‑1 (MQ=255)
                   tCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTg                                                  >  1:2342417/1‑71 (MQ=255)
                     tGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGtt                                                   <  1:2064173/68‑1 (MQ=255)
                       ggTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGtt                                                   <  1:859070/66‑1 (MQ=255)
                         tATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagc                                             >  1:1908517/1‑70 (MQ=255)
                           tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagcca                                           <  1:602215/70‑1 (MQ=255)
                           tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagcca                                           <  1:1781224/70‑1 (MQ=255)
                            cGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagccagc                                         >  1:2241864/1‑71 (MQ=255)
                             gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGt                                                                        <  1:1945258/39‑1 (MQ=255)
                             gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCg                                        >  1:646800/1‑71 (MQ=255)
                              tcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACg                                        <  1:193340/69‑1 (MQ=255)
                              ccGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCgt                                       <  1:3605062/71‑1 (MQ=255)
                               cGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctc                                                                    >  1:3751619/1‑41 (MQ=255)
                               cGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCgtg                                      <  1:3817949/71‑1 (MQ=255)
                               cGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCgtg                                      <  1:737673/71‑1 (MQ=255)
                                gATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagcca                                           >  1:3554393/1‑65 (MQ=255)
                                gATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTca                                               >  1:2322669/1‑61 (MQ=255)
                                gATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCgtgt                                     >  1:345795/1‑71 (MQ=255)
                                gATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCgtgt                                     >  1:584115/1‑71 (MQ=255)
                                 aTAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGc                                                             <  1:3161867/46‑1 (MQ=255)
                                 aTAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagcca                                           <  1:1569443/64‑1 (MQ=255)
                                  tAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTc                                   <  1:1020665/71‑1 (MQ=255)
                                  tAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTc                                   <  1:2633304/71‑1 (MQ=255)
                                    aCGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTcc                                  <  1:1988737/70‑1 (MQ=255)
                                     cGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGc                                                             <  1:1981437/42‑1 (MQ=255)
                                     cGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCgg                                <  1:422534/71‑1 (MQ=255)
                                      gACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                                   <  1:3050920/35‑1 (MQ=255)
                                            aTTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCg                                                          <  1:3698452/38‑1 (MQ=255)
                                              tGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGa                       >  1:120265/1‑71 (MQ=255)
                                                aaGTCTTCAACAGCAACGGTGCACTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGc                               <  1:2488119/61‑1 (MQ=255)
                                                 aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCgg                                <  1:2297830/59‑1 (MQ=255)
                                                 aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                    <  1:992511/71‑1 (MQ=255)
                                                 aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                    <  1:3099332/71‑1 (MQ=255)
                                                 aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                    <  1:2098452/71‑1 (MQ=255)
                                                 aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                    <  1:3534834/71‑1 (MQ=255)
                                                  gtattCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCat                   <  1:2917968/68‑1 (MQ=255)
                                                  gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                   >  1:3940907/1‑71 (MQ=255)
                                                  gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                   <  1:1371996/71‑1 (MQ=255)
                                                     ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTcc                                  >  1:2716712/1‑53 (MQ=255)
                                                     ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgat                >  1:2106827/1‑71 (MQ=255)
                                                     ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                   <  1:1945452/68‑1 (MQ=255)
                                                     ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                   <  1:1572509/68‑1 (MQ=255)
                                                     atCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatga                 <  1:755669/69‑1 (MQ=255)
                                                      tCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg               >  1:2692629/1‑71 (MQ=255)
                                                       cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg               <  1:1467150/70‑1 (MQ=255)
                                                       cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg               <  1:2167323/70‑1 (MQ=255)
                                                       cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg               <  1:2265125/70‑1 (MQ=255)
                                                       cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatga                 <  1:1150338/68‑1 (MQ=255)
                                                         aCAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc            <  1:2522119/71‑1 (MQ=255)
                                                          cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAcc           <  1:465427/71‑1 (MQ=255)
                                                          cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc            >  1:1048619/1‑70 (MQ=255)
                                                          cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc            >  1:3352417/1‑70 (MQ=255)
                                                             cAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAc         <  1:1064500/70‑1 (MQ=255)
                                                              aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc       >  1:2375329/1‑71 (MQ=255)
                                                              aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc       <  1:2197837/71‑1 (MQ=255)
                                                               aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg      <  1:2606534/71‑1 (MQ=255)
                                                               aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg      <  1:2548474/71‑1 (MQ=255)
                                                                cGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg      <  1:999822/70‑1 (MQ=255)
                                                                cGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgcc      >  1:2651998/1‑69 (MQ=255)
                                                                   tGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg  <  1:1451599/71‑1 (MQ=255)
                                                                   |                                                                      
GTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGG  >  minE/834904‑835041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: