Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,992 T→G 14.4% V1150V (GTT→GTG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,9920TG14.4% 165.8 / 27.7 83V1150V (GTT→GTGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (25/46);  new base G (5/7);  total (30/53)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCC  >  minE/834923‑835060
                                                                     |                                                                    
tCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTg                                                                     >  1:2342417/1‑71 (MQ=255)
  tGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGtt                                                                      <  1:2064173/68‑1 (MQ=255)
    ggTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGtt                                                                      <  1:859070/66‑1 (MQ=255)
      tATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagc                                                                >  1:1908517/1‑70 (MQ=255)
        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagcca                                                              <  1:1781224/70‑1 (MQ=255)
        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagcca                                                              <  1:602215/70‑1 (MQ=255)
         cGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagccagc                                                            >  1:2241864/1‑71 (MQ=255)
          gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCg                                                           >  1:646800/1‑71 (MQ=255)
           tcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACg                                                           <  1:193340/69‑1 (MQ=255)
           ccGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCgt                                                          <  1:3605062/71‑1 (MQ=255)
            cGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCgtg                                                         <  1:737673/71‑1 (MQ=255)
            cGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCgtg                                                         <  1:3817949/71‑1 (MQ=255)
             gATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagcca                                                              >  1:3554393/1‑65 (MQ=255)
             gATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTca                                                                  >  1:2322669/1‑61 (MQ=255)
             gATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCgtgt                                                        >  1:584115/1‑71 (MQ=255)
             gATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCgtgt                                                        >  1:345795/1‑71 (MQ=255)
              aTAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagcca                                                              <  1:1569443/64‑1 (MQ=255)
               tAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTc                                                      <  1:2633304/71‑1 (MQ=255)
               tAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTc                                                      <  1:1020665/71‑1 (MQ=255)
                 aCGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTcc                                                     <  1:1988737/70‑1 (MQ=255)
                  cGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCgg                                                   <  1:422534/71‑1 (MQ=255)
                           tGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGa                                          >  1:120265/1‑71 (MQ=255)
                             aaGTCTTCAACAGCAACGGTGCACTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGc                                                  <  1:2488119/61‑1 (MQ=255)
                              aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCgg                                                   <  1:2297830/59‑1 (MQ=255)
                              aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                       <  1:3099332/71‑1 (MQ=255)
                              aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                       <  1:3534834/71‑1 (MQ=255)
                              aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                       <  1:2098452/71‑1 (MQ=255)
                              aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                       <  1:992511/71‑1 (MQ=255)
                               gtattCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCat                                      <  1:2917968/68‑1 (MQ=255)
                               gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                      >  1:3940907/1‑71 (MQ=255)
                               gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                      <  1:1371996/71‑1 (MQ=255)
                                  ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTcc                                                     >  1:2716712/1‑53 (MQ=255)
                                  ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgat                                   >  1:2106827/1‑71 (MQ=255)
                                  ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                      <  1:1945452/68‑1 (MQ=255)
                                  ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                      <  1:1572509/68‑1 (MQ=255)
                                  atCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatga                                    <  1:755669/69‑1 (MQ=255)
                                   tCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                  >  1:2692629/1‑71 (MQ=255)
                                    cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                  <  1:2265125/70‑1 (MQ=255)
                                    cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                  <  1:2167323/70‑1 (MQ=255)
                                    cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                  <  1:1467150/70‑1 (MQ=255)
                                    cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatga                                    <  1:1150338/68‑1 (MQ=255)
                                      aCAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                               <  1:2522119/71‑1 (MQ=255)
                                       cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAcc                              <  1:465427/71‑1 (MQ=255)
                                       cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                               >  1:3352417/1‑70 (MQ=255)
                                       cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                               >  1:1048619/1‑70 (MQ=255)
                                          cAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAc                            <  1:1064500/70‑1 (MQ=255)
                                           aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc                          >  1:2375329/1‑71 (MQ=255)
                                           aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc                          <  1:2197837/71‑1 (MQ=255)
                                            aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                         <  1:2548474/71‑1 (MQ=255)
                                            aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                         <  1:2606534/71‑1 (MQ=255)
                                             cGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                         <  1:999822/70‑1 (MQ=255)
                                             cGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgcc                         >  1:2651998/1‑69 (MQ=255)
                                                tGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg                     <  1:1451599/71‑1 (MQ=255)
                                                 gCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg                     >  1:2691125/1‑70 (MQ=255)
                                                  ctctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCTCAGGa                    <  1:2769790/70‑1 (MQ=255)
                                                  ctctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGa                    <  1:3419235/70‑1 (MQ=255)
                                                    ctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg                 >  1:2245694/1‑71 (MQ=255)
                                                    ctGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCatg                                     <  1:1404635/51‑1 (MQ=255)
                                                     tGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg                 >  1:2452496/1‑70 (MQ=255)
                                                       aCTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa               <  1:3343759/70‑1 (MQ=255)
                                                       aCTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTAt              <  1:3157499/71‑1 (MQ=255)
                                                       aCTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTAt              <  1:2340125/71‑1 (MQ=255)
                                                       aCTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAt              >  1:2705619/1‑71 (MQ=255)
                                                        cTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                               >  1:3412902/1‑37 (MQ=255)
                                                        cTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATc             <  1:3439265/71‑1 (MQ=255)
                                                        cTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAtt             <  1:2391046/71‑2 (MQ=255)
                                                        cTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAtt             <  1:1094271/71‑2 (MQ=255)
                                                        cTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAt              >  1:1391483/1‑70 (MQ=255)
                                                         tGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg            >  1:3326240/1‑71 (MQ=255)
                                                         tGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg            >  1:1554806/1‑71 (MQ=255)
                                                           cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt          <  1:3282058/71‑1 (MQ=255)
                                                           cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt          <  1:3189789/71‑1 (MQ=255)
                                                           cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt          <  1:3185670/71‑1 (MQ=255)
                                                            ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa         <  1:2400344/71‑1 (MQ=255)
                                                            ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa         <  1:1291355/71‑1 (MQ=255)
                                                             cGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatg                                     <  1:3121471/42‑1 (MQ=255)
                                                              gTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAc       >  1:243385/1‑71 (MQ=255)
                                                               tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                  <  1:2912819/43‑1 (MQ=255)
                                                               tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa                                <  1:71734/45‑1 (MQ=255)
                                                               tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                         <  1:1470713/52‑1 (MQ=255)
                                                               tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc      >  1:218630/1‑71 (MQ=255)
                                                               tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc      >  1:3059265/1‑71 (MQ=255)
                                                               cgCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGaa                   >  1:3400297/2‑58 (MQ=255)
                                                                 cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg    >  1:3925061/1‑71 (MQ=255)
                                                                 cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg    <  1:104930/71‑1 (MQ=255)
                                                                    ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                         <  1:1695276/47‑1 (MQ=255)
                                                                    ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt                <  1:1402295/56‑1 (MQ=255)
                                                                    ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt                <  1:2749344/56‑1 (MQ=255)
                                                                    ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa               >  1:1742048/1‑57 (MQ=255)
                                                                    ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcc  <  1:456491/70‑1 (MQ=255)
                                                                     |                                                                    
TCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCC  >  minE/834923‑835060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: