Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 835,011 G→C 16.7% A1157P (GCA→CCA) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE835,0110GC16.7% 217.4 / 31.6 90A1157P (GCA→CCA) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (29/46);  new base C (7/8);  total (36/54)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAA  >  minE/834941‑835079
                                                                      |                                                                    
cGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCgg                                                                      <  1:422534/71‑1 (MQ=255)
         tGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGa                                                             >  1:120265/1‑71 (MQ=255)
           aaGTCTTCAACAGCAACGGTGCACTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGc                                                                     <  1:2488119/61‑1 (MQ=255)
            aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCgg                                                                      <  1:2297830/59‑1 (MQ=255)
            aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                                          <  1:992511/71‑1 (MQ=255)
            aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                                          <  1:2098452/71‑1 (MQ=255)
            aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                                          <  1:3099332/71‑1 (MQ=255)
            aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                                          <  1:3534834/71‑1 (MQ=255)
             gtattCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCat                                                         <  1:2917968/68‑1 (MQ=255)
             gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                                         <  1:1371996/71‑1 (MQ=255)
             gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                                         >  1:3940907/1‑71 (MQ=255)
                ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgat                                                      >  1:2106827/1‑71 (MQ=255)
                ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                                         <  1:1945452/68‑1 (MQ=255)
                ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                                         <  1:1572509/68‑1 (MQ=255)
                atCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatga                                                       <  1:755669/69‑1 (MQ=255)
                 tCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                                     >  1:2692629/1‑71 (MQ=255)
                  cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                                     <  1:2265125/70‑1 (MQ=255)
                  cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                                     <  1:2167323/70‑1 (MQ=255)
                  cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                                     <  1:1467150/70‑1 (MQ=255)
                  cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatga                                                       <  1:1150338/68‑1 (MQ=255)
                    aCAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                                                  <  1:2522119/71‑1 (MQ=255)
                     cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAcc                                                 <  1:465427/71‑1 (MQ=255)
                     cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                                                  >  1:3352417/1‑70 (MQ=255)
                     cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                                                  >  1:1048619/1‑70 (MQ=255)
                        cAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAc                                               <  1:1064500/70‑1 (MQ=255)
                         aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc                                             >  1:2375329/1‑71 (MQ=255)
                         aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc                                             <  1:2197837/71‑1 (MQ=255)
                          aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                                            <  1:2606534/71‑1 (MQ=255)
                          aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                                            <  1:2548474/71‑1 (MQ=255)
                           cGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                                            <  1:999822/70‑1 (MQ=255)
                           cGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgcc                                            >  1:2651998/1‑69 (MQ=255)
                              tGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg                                        <  1:1451599/71‑1 (MQ=255)
                               gCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg                                        >  1:2691125/1‑70 (MQ=255)
                                ctctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCTCAGGa                                       <  1:2769790/70‑1 (MQ=255)
                                ctctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGa                                       <  1:3419235/70‑1 (MQ=255)
                                  ctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg                                    >  1:2245694/1‑71 (MQ=255)
                                  ctGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCatg                                                        <  1:1404635/51‑1 (MQ=255)
                                   tGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg                                    >  1:2452496/1‑70 (MQ=255)
                                     aCTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa                                  <  1:3343759/70‑1 (MQ=255)
                                     aCTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTAt                                 <  1:3157499/71‑1 (MQ=255)
                                     aCTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTAt                                 <  1:2340125/71‑1 (MQ=255)
                                     aCTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAt                                 >  1:2705619/1‑71 (MQ=255)
                                      cTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                                  >  1:3412902/1‑37 (MQ=255)
                                      cTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATc                                <  1:3439265/71‑1 (MQ=255)
                                      cTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAtt                                <  1:2391046/71‑2 (MQ=255)
                                      cTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAtt                                <  1:1094271/71‑2 (MQ=255)
                                      cTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAt                                 >  1:1391483/1‑70 (MQ=255)
                                       tGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg                               >  1:3326240/1‑71 (MQ=255)
                                       tGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg                               >  1:1554806/1‑71 (MQ=255)
                                         cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt                             <  1:3282058/71‑1 (MQ=255)
                                         cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt                             <  1:3185670/71‑1 (MQ=255)
                                         cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt                             <  1:3189789/71‑1 (MQ=255)
                                          ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa                            <  1:2400344/71‑1 (MQ=255)
                                          ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa                            <  1:1291355/71‑1 (MQ=255)
                                           cGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatg                                                        <  1:3121471/42‑1 (MQ=255)
                                            gTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAc                          >  1:243385/1‑71 (MQ=255)
                                             tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                                     <  1:2912819/43‑1 (MQ=255)
                                             tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa                                                   <  1:71734/45‑1 (MQ=255)
                                             tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                                            <  1:1470713/52‑1 (MQ=255)
                                             tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc                         >  1:218630/1‑71 (MQ=255)
                                             tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc                         >  1:3059265/1‑71 (MQ=255)
                                             cgCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGaa                                      >  1:3400297/2‑58 (MQ=255)
                                               cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg                       >  1:3925061/1‑71 (MQ=255)
                                               cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg                       <  1:104930/71‑1 (MQ=255)
                                                  ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                                            <  1:1695276/47‑1 (MQ=255)
                                                  ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt                                   <  1:2749344/56‑1 (MQ=255)
                                                  ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt                                   <  1:1402295/56‑1 (MQ=255)
                                                  ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa                                  >  1:1742048/1‑57 (MQ=255)
                                                  ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcc                     <  1:456491/70‑1 (MQ=255)
                                                    gTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCa                                          >  1:2524787/1‑47 (MQ=255)
                                                     tCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGt                 <  1:2091218/71‑1 (MQ=255)
                                                     tCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACgca                                  <  1:3557089/54‑3 (MQ=16)
                                                       agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc               >  1:3278299/1‑71 (MQ=255)
                                                       agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc               >  1:3273817/1‑71 (MQ=255)
                                                       agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc               >  1:1489476/1‑71 (MQ=255)
                                                       agccaacGTGTACCGCCGGGCATGACCATGATGTATCAcgccc                                           >  1:50181/1‑41 (MQ=12)
                                                        ggcagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa                            <  1:1995826/55‑1 (MQ=255)
                                                        gccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACgca                                  <  1:1784727/51‑3 (MQ=16)
                                                         ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg             <  1:835364/71‑1 (MQ=255)
                                                         ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCg              >  1:525714/1‑70 (MQ=255)
                                                          cagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa            <  1:3403729/71‑1 (MQ=255)
                                                          cagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa            <  1:3941251/71‑1 (MQ=255)
                                                           agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAAc                                     >  1:2446594/1‑45 (MQ=255)
                                                           agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCGTGGTTCGGa            >  1:3309073/1‑70 (MQ=255)
                                                           agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa            >  1:2686197/1‑70 (MQ=255)
                                                            gcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa          >  1:1025423/1‑71 (MQ=255)
                                                            gcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa          >  1:452458/1‑71 (MQ=255)
                                                            gcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaa           >  1:2592330/1‑70 (MQ=255)
                                                            acGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAAc                                     >  1:341739/2‑44 (MQ=12)
                                                               tgtTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg                               <  1:1959701/47‑1 (MQ=255)
                                                                 ttCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAcc     <  1:3672553/71‑1 (MQ=255)
                                                                  tCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAcc     >  1:1647102/1‑70 (MQ=255)
                                                                      gCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCaa  >  1:1402724/1‑69 (MQ=255)
                                                                      |                                                                    
CGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAA  >  minE/834941‑835079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: