| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,013 | A→G | 16.5% | A1157A (GCA→GCG) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,013 | 0 | A | G | 16.5% | 214.9 / 32.6 | 91 | A1157A (GCA→GCG) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (31/45); new base G (7/8); total (38/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
TGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAA > minE/834950‑835079 | tGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGa > 1:120265/1‑71 (MQ=255) aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa < 1:2098452/71‑1 (MQ=255) aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa < 1:992511/71‑1 (MQ=255) aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa < 1:3099332/71‑1 (MQ=255) aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa < 1:3534834/71‑1 (MQ=255) gtattCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCat < 1:2917968/68‑1 (MQ=255) gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat < 1:1371996/71‑1 (MQ=255) gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat > 1:3940907/1‑71 (MQ=255) ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgat > 1:2106827/1‑71 (MQ=255) ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat < 1:1945452/68‑1 (MQ=255) ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat < 1:1572509/68‑1 (MQ=255) atCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatga < 1:755669/69‑1 (MQ=255) tCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg > 1:2692629/1‑71 (MQ=255) cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg < 1:2167323/70‑1 (MQ=255) cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg < 1:1467150/70‑1 (MQ=255) cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg < 1:2265125/70‑1 (MQ=255) cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatga < 1:1150338/68‑1 (MQ=255) aCAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc < 1:2522119/71‑1 (MQ=255) cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAcc < 1:465427/71‑1 (MQ=255) cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc > 1:1048619/1‑70 (MQ=255) cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc > 1:3352417/1‑70 (MQ=255) cAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAc < 1:1064500/70‑1 (MQ=255) aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc > 1:2375329/1‑71 (MQ=255) aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc < 1:2197837/71‑1 (MQ=255) aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg < 1:2548474/71‑1 (MQ=255) aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg < 1:2606534/71‑1 (MQ=255) cGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg < 1:999822/70‑1 (MQ=255) cGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgcc > 1:2651998/1‑69 (MQ=255) tGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg < 1:1451599/71‑1 (MQ=255) gCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg > 1:2691125/1‑70 (MQ=255) ctctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCTCAGGa < 1:2769790/70‑1 (MQ=255) ctctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGa < 1:3419235/70‑1 (MQ=255) ctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg > 1:2245694/1‑71 (MQ=255) ctGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCatg < 1:1404635/51‑1 (MQ=255) tGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg > 1:2452496/1‑70 (MQ=255) aCTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa < 1:3343759/70‑1 (MQ=255) aCTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTAt < 1:2340125/71‑1 (MQ=255) aCTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTAt < 1:3157499/71‑1 (MQ=255) aCTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAt > 1:2705619/1‑71 (MQ=255) cTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg > 1:3412902/1‑37 (MQ=255) cTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATc < 1:3439265/71‑1 (MQ=255) cTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAtt < 1:2391046/71‑2 (MQ=255) cTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAtt < 1:1094271/71‑2 (MQ=255) cTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAt > 1:1391483/1‑70 (MQ=255) tGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg > 1:1554806/1‑71 (MQ=255) tGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg > 1:3326240/1‑71 (MQ=255) cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt < 1:3185670/71‑1 (MQ=255) cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt < 1:3189789/71‑1 (MQ=255) cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt < 1:3282058/71‑1 (MQ=255) ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa < 1:1291355/71‑1 (MQ=255) ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa < 1:2400344/71‑1 (MQ=255) cGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatg < 1:3121471/42‑1 (MQ=255) gTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAc > 1:243385/1‑71 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg < 1:2912819/43‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa < 1:71734/45‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg < 1:1470713/52‑1 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc > 1:3059265/1‑71 (MQ=255) tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc > 1:218630/1‑71 (MQ=255) cgCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGaa > 1:3400297/2‑58 (MQ=255) cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg < 1:104930/71‑1 (MQ=255) cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg > 1:3925061/1‑71 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg < 1:1695276/47‑1 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt < 1:2749344/56‑1 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt < 1:1402295/56‑1 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa > 1:1742048/1‑57 (MQ=255) ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcc < 1:456491/70‑1 (MQ=255) gTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCa > 1:2524787/1‑47 (MQ=255) tCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGt < 1:2091218/71‑1 (MQ=255) tCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACgca < 1:3557089/54‑3 (MQ=16) agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc > 1:3278299/1‑71 (MQ=255) agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc > 1:3273817/1‑71 (MQ=255) agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc > 1:1489476/1‑71 (MQ=255) agccaacGTGTACCGCCGGGCATGACCATGATGTATCAcgccc > 1:50181/1‑41 (MQ=12) ggcagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa < 1:1995826/55‑1 (MQ=255) gccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACgca < 1:1784727/51‑3 (MQ=16) ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg < 1:835364/71‑1 (MQ=255) ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCg > 1:525714/1‑70 (MQ=255) cagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa < 1:3403729/71‑1 (MQ=255) cagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa < 1:3941251/71‑1 (MQ=255) agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAAc > 1:2446594/1‑45 (MQ=255) agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCGTGGTTCGGa > 1:3309073/1‑70 (MQ=255) agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa > 1:2686197/1‑70 (MQ=255) gcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa > 1:1025423/1‑71 (MQ=255) gcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa > 1:452458/1‑71 (MQ=255) gcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaa > 1:2592330/1‑70 (MQ=255) acGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAAc > 1:341739/2‑44 (MQ=12) tgtTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg < 1:1959701/47‑1 (MQ=255) ttCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAcc < 1:3672553/71‑1 (MQ=255) tCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAcc > 1:1647102/1‑70 (MQ=255) gCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCaa > 1:1402724/1‑69 (MQ=255) cAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAcc > 1:221613/1‑65 (MQ=255) | TGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAA > minE/834950‑835079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |