| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 190,078 | A→T | 47.5% | V525V (GTA→GTT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 190,078 | 0 | A | T | 47.5% | 8.0 / 56.4 | 40 | V525V (GTA→GTT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (11/10); new base T (3/16); total (14/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGG > minE/190030‑190145 | cccGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAAcc > 1:1743702/1‑71 (MQ=255) ccGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACct < 1:2744473/71‑1 (MQ=255) ggcggcGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGcc < 1:2081397/59‑1 (MQ=255) ggcggcGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACctgc > 1:728347/1‑71 (MQ=255) cTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCg > 1:1643744/1‑49 (MQ=255) gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCtt > 1:3645604/1‑71 (MQ=255) gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCtt < 1:77913/71‑1 (MQ=255) gaaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACctgctg < 1:3118408/57‑1 (MQ=19) gaaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACctgctg < 1:742703/57‑1 (MQ=19) aaaaTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTa < 1:3716269/71‑1 (MQ=255) aaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGtata < 1:722563/49‑1 (MQ=12) aaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAt < 1:2288050/71‑1 (MQ=255) aaTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTAt > 1:1495476/1‑70 (MQ=255) aaTACCTCGACGATCATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc > 1:3036022/1‑70 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAt < 1:1999686/70‑1 (MQ=255) aTTCCTCGACGAACGTGGGATCGTAGTAGAGaaaa > 1:504756/1‑35 (MQ=255) aTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTtct < 1:460844/60‑1 (MQ=255) aTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg < 1:3616792/71‑1 (MQ=255) cTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTAt < 1:2701062/71‑1 (MQ=255) cTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTAt < 1:2073456/71‑1 (MQ=255) cgacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATc > 1:2661880/1‑70 (MQ=255) gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCg < 1:3073030/70‑1 (MQ=255) gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGa > 1:1524719/1‑71 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:498848/70‑1 (MQ=255) acgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAAc > 1:2580940/1‑41 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:742748/71‑1 (MQ=255) cgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt > 1:2295930/1‑70 (MQ=255) aaCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACctg < 1:1476725/41‑1 (MQ=255) aCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaaaa < 1:3567069/71‑1 (MQ=255) gTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACctg > 1:2180636/1‑38 (MQ=255) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCa < 1:1405728/71‑1 (MQ=255) ggcaTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:1101192/60‑1 (MQ=12) gcaTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaaa < 1:2785490/69‑1 (MQ=255) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:3696164/70‑1 (MQ=255) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:3691571/70‑1 (MQ=255) aTCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg > 1:39732/1‑52 (MQ=255) aTCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCaa < 1:92617/68‑1 (MQ=255) tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa < 1:3732692/71‑1 (MQ=255) tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa > 1:2204207/1‑71 (MQ=255) tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa < 1:1021879/71‑1 (MQ=255) gtagAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATgg < 1:720342/70‑1 (MQ=255) gtagAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATgg < 1:1373617/70‑1 (MQ=255) | CCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGG > minE/190030‑190145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |