| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,193 | T→G | 19.0% | S1217S (TCT→TCG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,193 | 0 | T | G | 19.0% | 167.0 / 51.7 | 100 | S1217S (TCT→TCG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (42/39); new base G (15/4); total (57/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
ACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATG > minE/835124‑835260 | aCCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTa < 1:2975098/71‑1 (MQ=255) ccGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTaa > 1:524933/1‑71 (MQ=255) ccGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGACTTTAACTACTATGGCACCGTAGGTTCTaa < 1:2054333/71‑1 (MQ=255) aCGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGaa > 1:821054/1‑68 (MQ=255) aCGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAAcc < 1:1736336/70‑1 (MQ=255) aCGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcg > 1:1386857/1‑71 (MQ=255) cGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACcgc > 1:1024265/1‑71 (MQ=255) cGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgc > 1:1413148/1‑71 (MQ=255) cGCATATGATAGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCAACGTAGGTTCTAACcgc > 1:2120364/1‑71 (MQ=255) atatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCGCCGTAGGTTCTAACCGCGAt < 1:3111744/71‑1 (MQ=255) tatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt > 1:2326822/1‑70 (MQ=255) atGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGAt > 1:2767866/1‑69 (MQ=255) tGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAg > 1:2430583/1‑71 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAg > 1:2385409/1‑71 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAg > 1:3775995/1‑71 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAg > 1:2004842/1‑71 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAg > 1:1928400/1‑71 (MQ=255) attggcggtTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGCTCGAACCGCGATGAGtt > 1:917970/4‑71 (MQ=255) tCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGttt < 1:2406389/71‑1 (MQ=255) ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGa > 1:1534694/1‑65 (MQ=255) ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt < 1:1211359/71‑1 (MQ=255) ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt < 1:467046/71‑1 (MQ=255) ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttg < 1:2815226/70‑1 (MQ=255) gcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt < 1:397028/62‑1 (MQ=255) cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:3315226/71‑1 (MQ=255) gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGTTTCTAACCGCGATGAGtt < 1:2175920/64‑1 (MQ=255) gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg > 1:1368259/1‑69 (MQ=255) gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa < 1:3072486/71‑1 (MQ=255) cTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAAcc < 1:2392088/53‑1 (MQ=255) cTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcg < 1:2689045/54‑1 (MQ=255) tATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt > 1:577755/1‑71 (MQ=255) aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgcg > 1:2537532/1‑54 (MQ=255) aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTg > 1:1307414/1‑71 (MQ=255) cccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt > 1:3937580/1‑53 (MQ=255) cccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:3111698/64‑1 (MQ=255) ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGc > 1:1738706/1‑68 (MQ=255) ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGc > 1:642834/1‑68 (MQ=255) ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTa < 1:1495627/71‑1 (MQ=255) cATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa < 1:1549713/63‑1 (MQ=255) aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTaa > 1:730364/1‑43 (MQ=255) aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:3531113/61‑1 (MQ=255) aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt > 1:470160/1‑68 (MQ=255) cTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAg > 1:1887257/1‑61 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt > 1:175682/1‑58 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt > 1:179754/1‑61 (MQ=255) ggCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATg < 1:1965582/47‑1 (MQ=255) gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:2804415/55‑1 (MQ=255) gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCg > 1:1782375/1‑62 (MQ=255) gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATg < 1:3848771/69‑1 (MQ=255) aTACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAg > 1:285395/1‑56 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:1845858/52‑1 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:3157522/1‑71 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaaga > 1:2413535/1‑70 (MQ=255) aCGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:2662020/1‑70 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc < 1:1441248/70‑1 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa > 1:3578172/1‑71 (MQ=255) ggCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa > 1:1272169/1‑70 (MQ=255) ggCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcgt > 1:1695585/1‑69 (MQ=255) cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:2013526/48‑1 (MQ=255) cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt > 1:3558716/1‑70 (MQ=255) cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt > 1:2849592/1‑70 (MQ=255) cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTg < 1:3580214/71‑1 (MQ=255) cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTg < 1:1633377/71‑1 (MQ=255) cTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGtta < 1:297796/71‑2 (MQ=255) ttAACTACTATGGCACCGTAGGTTCTAACCGCGATGa > 1:1594689/1‑37 (MQ=255) ttAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt > 1:1880774/1‑49 (MQ=255) ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc > 1:110190/1‑71 (MQ=255) ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc > 1:2657265/1‑71 (MQ=255) ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc > 1:2248875/1‑71 (MQ=255) atAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:314686/2‑47 (MQ=255) tAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACt > 1:3348933/1‑71 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg > 1:48316/1‑71 (MQ=255) actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcg < 1:1522885/63‑2 (MQ=255) actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTgg < 1:914913/71‑1 (MQ=255) actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTgg > 1:1627332/1‑71 (MQ=255) ctactaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggc > 1:3641095/1‑70 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:3456589/70‑1 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:1907475/70‑1 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:2382585/70‑1 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:3220800/70‑1 (MQ=255) ctaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggctg < 1:1429012/70‑4 (MQ=255) ctaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggctg > 1:2086464/1‑67 (MQ=255) acggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt > 1:2152391/3‑71 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc > 1:3636905/1‑55 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa > 1:2879013/1‑60 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa > 1:1677448/1‑60 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa > 1:543449/1‑60 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa > 1:3271910/1‑60 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt < 1:1095397/70‑1 (MQ=255) gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATg > 1:3240278/1‑47 (MQ=255) cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGAt < 1:1714743/45‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAGGAACATTGACTGGTTAGATGGCGaa < 1:487614/71‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa < 1:3599427/51‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa < 1:725893/71‑1 (MQ=255) tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGc < 1:464788/71‑1 (MQ=255) gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt < 1:1175320/54‑1 (MQ=255) ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa < 1:1097543/42‑1 (MQ=255) ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATg < 1:1214518/71‑1 (MQ=255) tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg > 1:1144809/1‑58 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt > 1:82640/1‑67 (MQ=255) | ACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATG > minE/835124‑835260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |