Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 835,209 G→A 15.3% V1223I (GTT→ATT) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE835,2090GA15.3% 210.6 / 25.8 85V1223I (GTT→ATT) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (37/35);  new base A (9/4);  total (46/39)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 103 total aligned reads displayed.

GGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG  >  minE/835140‑835278
                                                                     |                                                                     
ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt                                                                      <  1:1211359/71‑1 (MQ=255)
ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt                                                                      <  1:467046/71‑1 (MQ=255)
ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttg                                                                       <  1:2815226/70‑1 (MQ=255)
  cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg                                                                    <  1:3315226/71‑1 (MQ=255)
    gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg                                                                    >  1:1368259/1‑69 (MQ=255)
    gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa                                                                  <  1:3072486/71‑1 (MQ=255)
      tATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt                                                                >  1:577755/1‑71 (MQ=255)
       aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTg                                                               >  1:1307414/1‑71 (MQ=255)
          cccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt                                                                   <  1:3111698/64‑1 (MQ=255)
           ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGc                                                              >  1:1738706/1‑68 (MQ=255)
           ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGc                                                              >  1:642834/1‑68 (MQ=255)
           ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTa                                                           <  1:1495627/71‑1 (MQ=255)
            cATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa                                                                  <  1:1549713/63‑1 (MQ=255)
             aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt                                                                   <  1:3531113/61‑1 (MQ=255)
             aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt                                                            >  1:470160/1‑68 (MQ=255)
               cTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAg                                                                 >  1:1887257/1‑61 (MQ=255)
                tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt                                                                   >  1:175682/1‑58 (MQ=255)
                tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt                                                                >  1:179754/1‑61 (MQ=255)
                  gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg                                                                    <  1:2804415/55‑1 (MQ=255)
                  gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCg                                                             >  1:1782375/1‑62 (MQ=255)
                  gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATg                                                      <  1:3848771/69‑1 (MQ=255)
                    aTACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAg                                                                 >  1:285395/1‑56 (MQ=255)
                     tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg                                                                    <  1:1845858/52‑1 (MQ=255)
                     tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa                                                 >  1:3157522/1‑71 (MQ=255)
                     tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaaga                                                  >  1:2413535/1‑70 (MQ=255)
                      aCGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa                                                 >  1:2662020/1‑70 (MQ=255)
                       cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc                                                <  1:1441248/70‑1 (MQ=255)
                       cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa                                               >  1:3578172/1‑71 (MQ=255)
                        ggCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa                                               >  1:1272169/1‑70 (MQ=255)
                        ggCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcgt                                              >  1:1695585/1‑69 (MQ=255)
                          cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt                                                                   <  1:2013526/48‑1 (MQ=255)
                          cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                             >  1:2849592/1‑70 (MQ=255)
                          cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                             >  1:3558716/1‑70 (MQ=255)
                          cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTg                                            <  1:1633377/71‑1 (MQ=255)
                          cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTg                                            <  1:3580214/71‑1 (MQ=255)
                          cTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGtta                                            <  1:297796/71‑2 (MQ=255)
                            ttAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt                                                                >  1:1880774/1‑49 (MQ=255)
                            ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc                                          >  1:110190/1‑71 (MQ=255)
                            ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc                                          >  1:2657265/1‑71 (MQ=255)
                            ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc                                          >  1:2248875/1‑71 (MQ=255)
                            atAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa                                                                  >  1:314686/2‑47 (MQ=255)
                             tAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACt                                         >  1:3348933/1‑71 (MQ=255)
                              aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg                                        >  1:48316/1‑71 (MQ=255)
                               actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcg                                               <  1:1522885/63‑2 (MQ=255)
                               actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTgg                                       >  1:1627332/1‑71 (MQ=255)
                               actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTgg                                       <  1:914913/71‑1 (MQ=255)
                                ctactaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggc                                      >  1:3641095/1‑70 (MQ=255)
                                   ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                    <  1:1907475/70‑1 (MQ=255)
                                   ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                    <  1:3220800/70‑1 (MQ=255)
                                   ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                    <  1:2382585/70‑1 (MQ=255)
                                   ctaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggctg                                    <  1:1429012/70‑4 (MQ=255)
                                   ctaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggctg                                    >  1:2086464/1‑67 (MQ=255)
                                     acggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt                                 >  1:2152391/3‑71 (MQ=255)
                                      tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc                                                >  1:3636905/1‑55 (MQ=255)
                                      tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa                                           >  1:2879013/1‑60 (MQ=255)
                                      tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa                                           >  1:3271910/1‑60 (MQ=255)
                                      tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa                                           >  1:543449/1‑60 (MQ=255)
                                      tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa                                           >  1:1677448/1‑60 (MQ=255)
                                      tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt                                 <  1:1095397/70‑1 (MQ=255)
                                        gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATg                                                      >  1:3240278/1‑47 (MQ=255)
                                         cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGAt                                                       <  1:1714743/45‑1 (MQ=255)
                                           ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAGGAACATTGACTGGTTAGATGGCGaa                           <  1:487614/71‑1 (MQ=255)
                                           ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa                                               <  1:3599427/51‑1 (MQ=255)
                                           ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa                           <  1:725893/71‑1 (MQ=255)
                                              tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGc                        <  1:464788/71‑1 (MQ=255)
                                                 gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt                                      <  1:1175320/54‑1 (MQ=255)
                                                  ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa                                                 <  1:1097543/42‑1 (MQ=255)
                                                  ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATg                    <  1:1214518/71‑1 (MQ=255)
                                                   tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg                                >  1:1144809/1‑58 (MQ=255)
                                                     tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                     >  1:82640/1‑67 (MQ=255)
                                                      aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATgaaga                                                  >  1:2092632/1‑37 (MQ=255)
                                                      aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATgaaga                                                  >  1:417241/1‑37 (MQ=255)
                                                      aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                     <  1:2957897/66‑1 (MQ=255)
                                                       aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                             <  1:701564/41‑1 (MQ=255)
                                                       aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                             <  1:1961004/41‑1 (MQ=255)
                                                       aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                             <  1:645685/41‑1 (MQ=255)
                                                       aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg                                >  1:2595832/1‑54 (MQ=255)
                                                       aCCGAGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                     >  1:2624426/1‑65 (MQ=255)
                                                        ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                     >  1:1615579/1‑64 (MQ=255)
                                                        ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAg               <  1:3194256/70‑1 (MQ=255)
                                                         cgcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg                                        >  1:3157168/1‑44 (MQ=255)
                                                         cgcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGt             <  1:3802636/71‑1 (MQ=255)
                                                            gatgatTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACa          <  1:652433/71‑1 (MQ=255)
                                                            gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                            <  1:3274004/53‑1 (MQ=255)
                                                            gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                            <  1:3853295/53‑1 (MQ=255)
                                                            gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                            <  1:370518/53‑1 (MQ=255)
                                                             aTGAGTTTGTTGTAGTGTGTAAGATGAAGAACATTGACTg                                        <  1:251812/40‑1 (MQ=255)
                                                             aTGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg         <  1:853548/71‑1 (MQ=255)
                                                               gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt                                      >  1:1693123/1‑40 (MQ=255)
                                                               gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt                                      >  1:1997888/1‑40 (MQ=255)
                                                                aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCaa                      >  1:3146941/1‑55 (MQ=255)
                                                                aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga       <  1:3819905/70‑1 (MQ=255)
                                                                aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga       <  1:1839570/70‑1 (MQ=255)
                                                                   ttgttgTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGgaga     >  1:2702740/1‑69 (MQ=255)
                                                                   ttgttgTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGgaga     >  1:780615/1‑69 (MQ=255)
                                                                    tgttgtAGTGCGTAAGATGAAGAACATTGACTGGtt                                     <  1:3509032/36‑1 (MQ=255)
                                                                    tgttgtAGTGCGTAAGATGAAGAACATTGACTGGtt                                     <  1:3228479/36‑1 (MQ=255)
                                                                    tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa                           >  1:3896716/1‑46 (MQ=255)
                                                                    tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg  >  1:541158/1‑71 (MQ=255)
                                                                     gttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg  >  1:3855325/1‑70 (MQ=255)
                                                                     |                                                                     
GGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG  >  minE/835140‑835278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: