Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 835,215 G→A 13.0% V1225M (GTG→ATG) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE835,2150GA13.0% 265.2 / 24.8 100V1225M (GTG→ATG) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (47/40);  new base A (9/4);  total (56/44)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 102 total aligned reads displayed.

TATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAAT  >  minE/835146‑835284
                                                                     |                                                                     
tATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt                                                                      >  1:577755/1‑71 (MQ=255)
 aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTg                                                                     >  1:1307414/1‑71 (MQ=255)
     ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGc                                                                    >  1:642834/1‑68 (MQ=255)
     ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGc                                                                    >  1:1738706/1‑68 (MQ=255)
     ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTa                                                                 <  1:1495627/71‑1 (MQ=255)
       aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt                                                                  >  1:470160/1‑68 (MQ=255)
         cTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAg                                                                       >  1:1887257/1‑61 (MQ=255)
          tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt                                                                      >  1:179754/1‑61 (MQ=255)
            gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCg                                                                   >  1:1782375/1‑62 (MQ=255)
            gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATg                                                            <  1:3848771/69‑1 (MQ=255)
              aTACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAg                                                                       >  1:285395/1‑56 (MQ=255)
               tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa                                                       >  1:3157522/1‑71 (MQ=255)
               tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaaga                                                        >  1:2413535/1‑70 (MQ=255)
                aCGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa                                                       >  1:2662020/1‑70 (MQ=255)
                 cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc                                                      <  1:1441248/70‑1 (MQ=255)
                 cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa                                                     >  1:3578172/1‑71 (MQ=255)
                  ggCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa                                                     >  1:1272169/1‑70 (MQ=255)
                  ggCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcgt                                                    >  1:1695585/1‑69 (MQ=255)
                    cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                                   >  1:2849592/1‑70 (MQ=255)
                    cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                                   >  1:3558716/1‑70 (MQ=255)
                    cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTg                                                  <  1:1633377/71‑1 (MQ=255)
                    cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTg                                                  <  1:3580214/71‑1 (MQ=255)
                      ttAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt                                                                      >  1:1880774/1‑49 (MQ=255)
                      ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc                                                >  1:2657265/1‑71 (MQ=255)
                      ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc                                                >  1:110190/1‑71 (MQ=255)
                      ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc                                                >  1:2248875/1‑71 (MQ=255)
                       tAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACt                                               >  1:3348933/1‑71 (MQ=255)
                        aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg                                              >  1:48316/1‑71 (MQ=255)
                         actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcg                                                     <  1:1522885/63‑2 (MQ=255)
                         actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTgg                                             <  1:914913/71‑1 (MQ=255)
                         actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTgg                                             >  1:1627332/1‑71 (MQ=255)
                          ctactaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggc                                            >  1:3641095/1‑70 (MQ=255)
                             ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                          <  1:1907475/70‑1 (MQ=255)
                             ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                          <  1:3456589/70‑1 (MQ=255)
                             ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                          <  1:3220800/70‑1 (MQ=255)
                             ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                          <  1:2382585/70‑1 (MQ=255)
                             ctaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggctg                                          >  1:2086464/1‑67 (MQ=255)
                             ctaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggctg                                          <  1:1429012/70‑4 (MQ=255)
                               acggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt                                       >  1:2152391/3‑71 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc                                                      >  1:3636905/1‑55 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa                                                 >  1:2879013/1‑60 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa                                                 >  1:1677448/1‑60 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa                                                 >  1:3271910/1‑60 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa                                                 >  1:543449/1‑60 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt                                       <  1:1095397/70‑1 (MQ=255)
                                  gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATg                                                            >  1:3240278/1‑47 (MQ=255)
                                   cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGAt                                                             <  1:1714743/45‑1 (MQ=255)
                                     ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAGGAACATTGACTGGTTAGATGGCGaa                                 <  1:487614/71‑1 (MQ=255)
                                     ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa                                                     <  1:3599427/51‑1 (MQ=255)
                                     ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa                                 <  1:725893/71‑1 (MQ=255)
                                        tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGc                              <  1:464788/71‑1 (MQ=255)
                                           gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt                                            <  1:1175320/54‑1 (MQ=255)
                                            ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa                                                       <  1:1097543/42‑1 (MQ=255)
                                            ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATg                          <  1:1214518/71‑1 (MQ=255)
                                             tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg                                      >  1:1144809/1‑58 (MQ=255)
                                               tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                           >  1:82640/1‑67 (MQ=255)
                                                aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATgaaga                                                        >  1:417241/1‑37 (MQ=255)
                                                aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATgaaga                                                        >  1:2092632/1‑37 (MQ=255)
                                                aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                           <  1:2957897/66‑1 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                                   <  1:701564/41‑1 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                                   <  1:645685/41‑1 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                                   <  1:1961004/41‑1 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg                                      <  1:2070912/54‑1 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg                                      >  1:2595832/1‑54 (MQ=255)
                                                 aCCGAGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                           >  1:2624426/1‑65 (MQ=255)
                                                  ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                           >  1:1615579/1‑64 (MQ=255)
                                                  ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAg                     <  1:3194256/70‑1 (MQ=255)
                                                   cgcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg                                              >  1:3157168/1‑44 (MQ=255)
                                                   cgcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGt                   <  1:3802636/71‑1 (MQ=255)
                                                      gatgatTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACa                <  1:652433/71‑1 (MQ=255)
                                                      gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                                  <  1:370518/53‑1 (MQ=255)
                                                      gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                                  <  1:3274004/53‑1 (MQ=255)
                                                      gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                                  <  1:3853295/53‑1 (MQ=255)
                                                       aTGAGTTTGTTGTAGTGTGTAAGATGAAGAACATTGACTg                                              <  1:251812/40‑1 (MQ=255)
                                                       aTGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg               <  1:853548/71‑1 (MQ=255)
                                                         gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt                                            >  1:1693123/1‑40 (MQ=255)
                                                         gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt                                            >  1:1997888/1‑40 (MQ=255)
                                                          aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCaa                            >  1:3146941/1‑55 (MQ=255)
                                                          aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga             <  1:3819905/70‑1 (MQ=255)
                                                          aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga             <  1:1839570/70‑1 (MQ=255)
                                                             ttgttgTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGgaga           >  1:2702740/1‑69 (MQ=255)
                                                             ttgttgTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGgaga           >  1:780615/1‑69 (MQ=255)
                                                              tgttgtAGTGCGTAAGATGAAGAACATTGACTGGtt                                           <  1:3228479/36‑1 (MQ=255)
                                                              tgttgtAGTGCGTAAGATGAAGAACATTGACTGGtt                                           <  1:3509032/36‑1 (MQ=255)
                                                              tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa                                 >  1:3896716/1‑46 (MQ=255)
                                                              tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg        >  1:541158/1‑71 (MQ=255)
                                                               gttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg        >  1:3855325/1‑70 (MQ=255)
                                                                 tgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg               >  1:3705822/1‑61 (MQ=255)
                                                                 tgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTa      >  1:3829155/1‑70 (MQ=255)
                                                                  gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGa                                        >  1:294759/1‑35 (MQ=255)
                                                                  gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt                                       >  1:79511/1‑36 (MQ=255)
                                                                  gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg               >  1:1157715/1‑60 (MQ=255)
                                                                  gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaa     <  1:2938315/70‑1 (MQ=255)
                                                                  gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaa     >  1:3759474/1‑70 (MQ=255)
                                                                  gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaa     >  1:328648/1‑70 (MQ=255)
                                                                   tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa                      <  1:1170022/52‑1 (MQ=255)
                                                                   tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa                      <  1:3943261/52‑1 (MQ=255)
                                                                   tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaaa    <  1:3301694/70‑1 (MQ=255)
                                                                    aGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaaaa   <  1:2017284/70‑1 (MQ=255)
                                                                    aGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAAt  >  1:3537989/1‑71 (MQ=255)
                                                                     |                                                                     
TATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAAT  >  minE/835146‑835284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: