| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,236 | G→A | 22.6% | D1232N (GAC→AAC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,236 | 0 | G | A | 22.6% | 179.0 / 48.7 | 93 | D1232N (GAC→AAC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (35/37); new base A (13/8); total (48/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01 | |||||||||||
CTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGG > minE/835166‑835305 | cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTg < 1:3580214/71‑1 (MQ=255)cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTg < 1:1633377/71‑1 (MQ=255) ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc > 1:2248875/1‑71 (MQ=255) ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc > 1:110190/1‑71 (MQ=255) ttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc > 1:2657265/1‑71 (MQ=255) tAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACt > 1:3348933/1‑71 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg > 1:48316/1‑71 (MQ=255) actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTgg < 1:914913/71‑1 (MQ=255) actactACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTgg > 1:1627332/1‑71 (MQ=255) ctactaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggc > 1:3641095/1‑70 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:2382585/70‑1 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:3220800/70‑1 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:1907475/70‑1 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:3456589/70‑1 (MQ=255) ctaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggctg < 1:1429012/70‑4 (MQ=255) ctaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggctg > 1:2086464/1‑67 (MQ=255) acggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt > 1:2152391/3‑71 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa > 1:3271910/1‑60 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa > 1:1677448/1‑60 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa > 1:2879013/1‑60 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa > 1:543449/1‑60 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt < 1:1095397/70‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAGGAACATTGACTGGTTAGATGGCGaa < 1:487614/71‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa < 1:725893/71‑1 (MQ=255) tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGc < 1:464788/71‑1 (MQ=255) gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt < 1:1175320/54‑1 (MQ=255) ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATg < 1:1214518/71‑1 (MQ=255) tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg > 1:1144809/1‑58 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt > 1:82640/1‑67 (MQ=255) aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt < 1:2957897/66‑1 (MQ=255) aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg > 1:2595832/1‑54 (MQ=255) aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg < 1:2070912/54‑1 (MQ=255) aCCGAGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt > 1:2624426/1‑65 (MQ=255) ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt > 1:1615579/1‑64 (MQ=255) ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAg < 1:3194256/70‑1 (MQ=255) cgcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg > 1:3157168/1‑44 (MQ=255) cgcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGt < 1:3802636/71‑1 (MQ=255) gatgatTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACa < 1:652433/71‑1 (MQ=255) gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa < 1:3853295/53‑1 (MQ=255) gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa < 1:3274004/53‑1 (MQ=255) gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa < 1:370518/53‑1 (MQ=255) aTGAGTTTGTTGTAGTGTGTAAGATGAAGAACATTGACTg < 1:251812/40‑1 (MQ=255) aTGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg < 1:853548/71‑1 (MQ=255) gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt > 1:1997888/1‑40 (MQ=255) gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt > 1:1693123/1‑40 (MQ=255) gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg > 1:959944/1‑69 (MQ=255) aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCaa > 1:3146941/1‑55 (MQ=255) aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga < 1:1839570/70‑1 (MQ=255) aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga < 1:3819905/70‑1 (MQ=255) ttgttgTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGgaga > 1:2702740/1‑69 (MQ=255) ttgttgTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGgaga > 1:780615/1‑69 (MQ=255) tgttgtAGTGCGTAAGATGAAGAACATTGACTGGtt < 1:3228479/36‑1 (MQ=255) tgttgtAGTGCGTAAGATGAAGAACATTGACTGGtt < 1:3509032/36‑1 (MQ=255) tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa > 1:3896716/1‑46 (MQ=255) tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg > 1:541158/1‑71 (MQ=255) gttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg > 1:3855325/1‑70 (MQ=255) tgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg > 1:3705822/1‑61 (MQ=255) tgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Ta > 1:3829155/1‑70 (MQ=255) gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGa > 1:294759/1‑35 (MQ=255) gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt > 1:79511/1‑36 (MQ=255) gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg > 1:1157715/1‑60 (MQ=255) gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taa < 1:2938315/70‑1 (MQ=255) gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taa > 1:328648/1‑70 (MQ=255) gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taa > 1:3759474/1‑70 (MQ=255) tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa < 1:3943261/52‑1 (MQ=255) tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa < 1:1170022/52‑1 (MQ=255) tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaa < 1:3301694/70‑1 (MQ=255) aGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa < 1:2017284/70‑1 (MQ=255) aGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAAt > 1:3537989/1‑71 (MQ=255) tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTAc < 1:504547/54‑1 (MQ=255) tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTAc < 1:3371468/54‑1 (MQ=255) gCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGa > 1:2445047/1‑70 (MQ=255) cgcaaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGaaa < 1:2209642/68‑1 (MQ=255) cgcaaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGaaa < 1:1657235/68‑1 (MQ=255) cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAg > 1:2462320/1‑37 (MQ=255) gcaaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGga > 1:765425/3‑55 (MQ=255) tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGa < 1:3772670/67‑1 (MQ=255) tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAAt < 1:751456/71‑1 (MQ=255) tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGAG‑TAAAATGAAAAt < 1:3862461/71‑1 (MQ=255) caaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGgagg > 1:431940/2‑55 (MQ=255) caaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAAt > 1:2576504/2‑71 (MQ=255) caaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAAt > 1:3320715/2‑71 (MQ=255) aGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAAtcc > 1:2944020/1‑69 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATg > 1:138884/1‑39 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga > 1:2597219/1‑52 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc < 1:1001081/71‑1 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc > 1:614527/1‑71 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc > 1:3828491/1‑71 (MQ=255) gATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCg < 1:148799/71‑1 (MQ=255) gATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCg < 1:1923858/71‑1 (MQ=255) aagaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTca < 1:1018540/71‑1 (MQ=255) agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcac < 1:1445858/71‑1 (MQ=255) gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:1311226/70‑1 (MQ=255) aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc > 1:2275607/1‑62 (MQ=255) aTTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaaaa < 1:3940656/57‑1 (MQ=255) gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:1714093/70‑1 (MQ=255) gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:3132414/70‑1 (MQ=255) | CTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGG > minE/835166‑835305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |