| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,259 | T→C | 12.6% | N1239N (AAT→AAC) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,259 | 0 | T | C | 12.6% | 224.3 / 20.9 | 87 | N1239N (AAT→AAC) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (35/41); new base C (5/6); total (40/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
TTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTG > minE/835190‑835329 | ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATg < 1:1214518/71‑1 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt > 1:82640/1‑67 (MQ=255) aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt < 1:2957897/66‑1 (MQ=255) aCCGAGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt > 1:2624426/1‑65 (MQ=255) ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt > 1:1615579/1‑64 (MQ=255) ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAg < 1:3194256/70‑1 (MQ=255) cgcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGt < 1:3802636/71‑1 (MQ=255) gatgatTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACa < 1:652433/71‑1 (MQ=255) aTGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg < 1:853548/71‑1 (MQ=255) gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg > 1:959944/1‑69 (MQ=255) aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga < 1:3819905/70‑1 (MQ=255) aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga < 1:1839570/70‑1 (MQ=255) ttgttgTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGgaga > 1:780615/1‑69 (MQ=255) ttgttgTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGgaga > 1:2702740/1‑69 (MQ=255) tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg > 1:541158/1‑71 (MQ=255) gttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg > 1:3855325/1‑70 (MQ=255) tgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg > 1:3705822/1‑61 (MQ=255) tgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Ta > 1:3829155/1‑70 (MQ=255) gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg > 1:1157715/1‑60 (MQ=255) gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taa > 1:3759474/1‑70 (MQ=255) gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taa > 1:328648/1‑70 (MQ=255) gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taa < 1:2938315/70‑1 (MQ=255) tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa < 1:3943261/52‑1 (MQ=255) tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa < 1:1170022/52‑1 (MQ=255) tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaa < 1:3301694/70‑1 (MQ=255) aGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa < 1:2017284/70‑1 (MQ=255) aGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAAt > 1:3537989/1‑71 (MQ=255) tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTAc < 1:504547/54‑1 (MQ=255) tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTAc < 1:3371468/54‑1 (MQ=255) gCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGa > 1:2445047/1‑70 (MQ=255) cgcaaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGaaa < 1:1657235/68‑1 (MQ=255) cgcaaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGaaa < 1:2209642/68‑1 (MQ=255) gcaaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGga > 1:765425/3‑55 (MQ=255) tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGa < 1:3772670/67‑1 (MQ=255) tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAAt < 1:751456/71‑1 (MQ=255) tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGAG‑TAAAATGAAAAt < 1:3862461/71‑1 (MQ=255) caaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGgagg > 1:431940/2‑55 (MQ=255) caaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAAt > 1:3320715/2‑71 (MQ=255) caaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAAt > 1:2576504/2‑71 (MQ=255) aGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAAtcc > 1:2944020/1‑69 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATg > 1:138884/1‑39 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga > 1:2597219/1‑52 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc > 1:3828491/1‑71 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc > 1:614527/1‑71 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc < 1:1001081/71‑1 (MQ=255) gATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCg < 1:1923858/71‑1 (MQ=255) gATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCg < 1:148799/71‑1 (MQ=255) aagaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTca < 1:1018540/71‑1 (MQ=255) agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcac < 1:1445858/71‑1 (MQ=255) gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:1311226/70‑1 (MQ=255) aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc > 1:2275607/1‑62 (MQ=255) aTTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaaaa < 1:3940656/57‑1 (MQ=255) gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:3132414/70‑1 (MQ=255) gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:1714093/70‑1 (MQ=255) cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt > 1:1855724/1‑71 (MQ=255) cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt > 1:1350295/1‑71 (MQ=255) tGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc > 1:1875490/1‑54 (MQ=255) tGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt > 1:2995808/1‑57 (MQ=255) tGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg > 1:595237/1‑71 (MQ=255) gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATgg < 1:2762370/70‑1 (MQ=255) gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATgg < 1:1763620/70‑1 (MQ=255) gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATgg < 1:3760649/70‑1 (MQ=255) gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg < 1:1628791/69‑1 (MQ=255) gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGt > 1:2223794/1‑71 (MQ=255) gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGt > 1:2219106/1‑71 (MQ=255) gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGt < 1:3780801/71‑1 (MQ=255) ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTg > 1:3238161/1‑71 (MQ=255) tAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca > 1:2932218/1‑57 (MQ=255) tAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca > 1:1305391/1‑57 (MQ=255) aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaaaa < 1:484874/46‑1 (MQ=255) aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt > 1:265481/1‑71 (MQ=255) gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCa < 1:1642333/63‑1 (MQ=255) gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt < 1:3464655/70‑1 (MQ=255) gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:1154401/57‑1 (MQ=255) gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCa < 1:1689228/59‑1 (MQ=255) gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt < 1:3189656/70‑1 (MQ=255) cGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct > 1:3818181/1‑71 (MQ=255) cGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct < 1:1833539/71‑1 (MQ=255) gAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGa < 1:123019/36‑1 (MQ=255) gAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCa > 1:713048/1‑57 (MQ=255) aaGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtctc > 1:1295489/1‑70 (MQ=255) aGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct > 1:1676601/1‑68 (MQ=255) aGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:632673/1‑71 (MQ=255) aGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa < 1:665169/71‑1 (MQ=255) ggCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGTCATGGTGCTGAAtc < 1:1098645/66‑1 (MQ=255) gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGt < 1:2951322/40‑1 (MQ=255) cAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt > 1:2548940/1‑69 (MQ=255) cAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATaa < 1:2397116/71‑1 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt < 1:1026738/39‑1 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca > 1:3176505/1‑43 (MQ=255) aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATgg < 1:260240/53‑1 (MQ=255) tGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac > 1:1793006/1‑40 (MQ=255) tGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTg > 1:2816562/1‑71 (MQ=255) | TTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTG > minE/835190‑835329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |