| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,318 | T→C | 37.5% | N12N (AAT→AAC) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,318 | 0 | T | C | 37.5% | 48.3 / 63.2 | 64 | N12N (AAT→AAC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (13/27); new base C (15/9); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.63e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGAAGGCAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGC > minE/835249‑835387 | gCGAAGGCAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt < 1:3189656/70‑1 (MQ=255) cGAAGGCAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct > 1:3818181/1‑71 (MQ=255) cGAAGGCAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct < 1:1833539/71‑1 (MQ=255) aaGGCAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtctc > 1:1295489/1‑70 (MQ=255) aGGCAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct > 1:1676601/1‑68 (MQ=255) aGGCAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa < 1:665169/71‑1 (MQ=255) aGGCAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:632673/1‑71 (MQ=255) ggCAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGTCATGGTGCTGAAtc < 1:1098645/66‑1 (MQ=255) cAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt > 1:2548940/1‑69 (MQ=255) cAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATaa < 1:2397116/71‑1 (MQ=255) tGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTg > 1:2816562/1‑71 (MQ=255) tcAGGTACAGGAG‑GCGAA‑AAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt < 1:1038941/70‑1 (MQ=255) cAGGTACAGGAG‑GCGAA‑AAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:322274/1‑70 (MQ=255) aGGTACAGGAGAGTG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt < 1:3222746/69‑1 (MQ=255) aGGTACAGGAG‑GCGAA‑AAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCg > 1:580056/1‑71 (MQ=255) ggTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCgg < 1:2976711/71‑1 (MQ=255) gTACAGGAG‑GCG‑AAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGc > 1:3140096/1‑71 (MQ=255) aCAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa < 1:1988319/64‑1 (MQ=255) aCAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa < 1:3797985/64‑1 (MQ=255) cAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg < 1:2270425/70‑1 (MQ=255) gagaGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGcc > 1:3111585/1‑69 (MQ=255) agagCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGc < 1:549269/58‑1 (MQ=255) gagCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATaa > 1:681826/1‑53 (MQ=255) cG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTg < 1:1840511/71‑1 (MQ=255) gaaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTg < 1:1483715/68‑1 (MQ=255) aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:3036642/69‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:2435410/70‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:3089650/69‑1 (MQ=255) aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTg > 1:969075/1‑59 (MQ=255) aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt > 1:3637829/1‑70 (MQ=255) aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTc > 1:3290229/1‑71 (MQ=255) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTcg > 1:2703013/1‑70 (MQ=255) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTcg < 1:2871943/71‑2 (MQ=255) aaTGAAAATCCGTTCCCAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTcg < 1:3485491/70‑2 (MQ=255) aTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGt > 1:1483404/1‑71 (MQ=255) tGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGt > 1:2539524/1‑70 (MQ=255) aaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAc < 1:1770798/70‑1 (MQ=255) aaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTcg > 1:2777663/1‑64 (MQ=255) aaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGAcc > 1:2993079/1‑71 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCacac < 1:290989/57‑1 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCt < 1:1588623/71‑1 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:1946984/37‑1 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCGGAATCTCGATAAGTGCATCGGCTGc < 1:997862/51‑1 (MQ=255) ccGTTCTCAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGt > 1:507840/2‑70 (MQ=255) ccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTg > 1:1325990/2‑69 (MQ=255) ccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGt > 1:1927102/2‑70 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCt < 1:3455879/53‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCt < 1:405315/53‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaaaa < 1:1602300/71‑1 (MQ=255) tCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaaaa > 1:1401145/1‑71 (MQ=255) tCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCcac < 1:2654955/48‑1 (MQ=255) cacaAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGtt > 1:293543/1‑59 (MQ=255) acaAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAAc < 1:877137/70‑1 (MQ=255) acaAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACg > 1:3793689/1‑71 (MQ=255) acaAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACg > 1:214548/1‑71 (MQ=255) caAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGt < 1:1160162/71‑1 (MQ=255) aGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCt > 1:3677306/1‑71 (MQ=255) aGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCt > 1:3672907/1‑71 (MQ=255) aGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCt > 1:3855265/1‑71 (MQ=255) gTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTg < 1:3680397/71‑1 (MQ=255) gTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTg < 1:973566/71‑1 (MQ=255) gTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTg < 1:1312074/71‑1 (MQ=255) gTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTg < 1:1227733/71‑1 (MQ=255) tCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTgg < 1:3619794/71‑1 (MQ=255) gCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACATCTGTTCAGTTACCTGTAAAAACGTCTGGAcc < 1:1836487/71‑1 (MQ=255) ggTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGcc > 1:52784/1‑71 (MQ=255) gAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCt > 1:2396373/1‑56 (MQ=255) aTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGc < 1:70033/71‑1 (MQ=255) aTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGc < 1:2687975/71‑1 (MQ=255) aTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGc < 1:3043866/71‑1 (MQ=255) | GCGAAGGCAATGACCAGGTACAGGAGAGCG‑T‑AAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGC > minE/835249‑835387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |