Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 190,108 T→C 40.4% F535F (TTT→TTC ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE190,1080TC40.4% 25.8 / 49.8 47F535F (TTT→TTCldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base T (12/16);  new base C (6/13);  total (18/29)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCT  >  minE/190043‑190178
                                                                 |                                                                      
gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCtt                                                                   >  1:3645604/1‑71 (MQ=255)
gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCtt                                                                   <  1:77913/71‑1 (MQ=255)
     aaaaTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTa                                                              <  1:3716269/71‑1 (MQ=255)
      aaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAt                                                             <  1:2288050/71‑1 (MQ=255)
       aaTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTAt                                                             >  1:1495476/1‑70 (MQ=255)
       aaTACCTCGACGATCATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc                                                            >  1:3036022/1‑70 (MQ=255)
       aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAt                                                             <  1:1999686/70‑1 (MQ=255)
        aTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTtct                                                                      <  1:460844/60‑1 (MQ=255)
        aTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg                                                           <  1:3616792/71‑1 (MQ=255)
            cTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTAt                                                       <  1:2701062/71‑1 (MQ=255)
            cTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTAt                                                       <  1:2073456/71‑1 (MQ=255)
              cgacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATc                                                      >  1:2661880/1‑70 (MQ=255)
               gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCg                                                     <  1:3073030/70‑1 (MQ=255)
               gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGa                                                    >  1:1524719/1‑71 (MQ=255)
               gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg                                                     <  1:498848/70‑1 (MQ=255)
                acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt                                                   <  1:742748/71‑1 (MQ=255)
                 cgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt                                                   >  1:2295930/1‑70 (MQ=255)
                    aCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaaaa                                               <  1:3567069/71‑1 (MQ=255)
                       tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCa                                            <  1:1405728/71‑1 (MQ=255)
                        ggcaTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt                                                   <  1:1101192/60‑1 (MQ=12)
                         gcaTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaaa                                          <  1:2785490/69‑1 (MQ=255)
                          caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg                                         <  1:3696164/70‑1 (MQ=255)
                          caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg                                         <  1:3691571/70‑1 (MQ=255)
                           aTCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg                                                           >  1:39732/1‑52 (MQ=255)
                           aTCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCaa                                           <  1:92617/68‑1 (MQ=255)
                            tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa                                       <  1:1021879/71‑1 (MQ=255)
                            tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa                                       <  1:3732692/71‑1 (MQ=255)
                            tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa                                       >  1:2204207/1‑71 (MQ=255)
                                 gttgagaAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa                                           >  1:3158362/4‑62 (MQ=255)
                                 gtagAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATgg                                   <  1:720342/70‑1 (MQ=255)
                                 gtagAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATgg                                   <  1:1373617/70‑1 (MQ=255)
                                    gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAAt                                     >  1:2195073/1‑65 (MQ=255)
                                       aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg                                                           <  1:3842599/40‑1 (MQ=255)
                                       aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg                                                           <  1:2744420/40‑1 (MQ=255)
                                       aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg                                                           <  1:626228/40‑1 (MQ=255)
                                       aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGc                                        <  1:1483645/59‑1 (MQ=255)
                                       aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATgg                                   <  1:3110597/64‑1 (MQ=255)
                                           ccGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGt                        <  1:2901634/71‑1 (MQ=255)
                                            cGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg                       >  1:3067743/1‑71 (MQ=255)
                                                ccTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGtt                   >  1:2851227/1‑71 (MQ=255)
                                                ccTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGtt                   >  1:2788236/1‑71 (MQ=255)
                                                    ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGatta                              <  1:2108835/56‑1 (MQ=255)
                                                    ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg               >  1:1323502/1‑71 (MQ=255)
                                                    ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGACTATTGCGTGGGTTGACg               >  1:588582/1‑71 (MQ=255)
                                                    ataACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCac                                      >  1:1819542/1‑47 (MQ=255)
                                                    ataACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCac                                      >  1:955875/1‑47 (MQ=255)
                                                       aCCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAAt                                     >  1:3657983/1‑46 (MQ=255)
                                                         ctgctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACgg              <  1:1493677/67‑1 (MQ=255)
                                                               tttCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCaaa      <  1:2156852/69‑1 (MQ=255)
                                                                 tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGct  >  1:2558698/1‑71 (MQ=255)
                                                                 |                                                                      
GTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCT  >  minE/190043‑190178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: