| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,414 | G→C | 9.6% | V44V (GTG→GTC) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,414 | 0 | G | C | 9.6% | 142.4 / 5.8 | 52 | V44V (GTG→GTC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (24/23); new base C (3/2); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTG > minE/835345‑835479 | cTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTgg < 1:1327911/71‑1 (MQ=255)cTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTg > 1:3401816/1‑70 (MQ=255) ttgaGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGaa < 1:2134511/67‑1 (MQ=255) ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaa > 1:2118523/1‑71 (MQ=255) ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaa < 1:243508/70‑1 (MQ=255) ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGa < 1:2919966/69‑1 (MQ=255) ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa < 1:3215110/69‑1 (MQ=255) cAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAAc < 1:3762977/70‑1 (MQ=255) aGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCa < 1:1729499/71‑1 (MQ=255) gTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAAcc > 1:2873644/1‑69 (MQ=255) gTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCaa < 1:3256987/71‑1 (MQ=255) ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAAcc < 1:2143466/68‑1 (MQ=255) ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAg < 1:942218/71‑1 (MQ=255) aCCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaaccaaacc > 1:690504/1‑71 (MQ=255) aCCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCaa < 1:1861070/68‑1 (MQ=255) aCCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGcc > 1:3361058/1‑71 (MQ=255) ccTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGcc < 1:2274587/70‑1 (MQ=255) ttAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCggg > 1:3332404/2‑70 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGcc < 1:3938006/71‑1 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGcc < 1:188282/71‑1 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGc > 1:1180552/1‑70 (MQ=255) aaaaCGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCGGGcatt > 1:1561972/1‑68 (MQ=255) aaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCa < 1:3446346/70‑1 (MQ=255) aCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGc > 1:1035147/1‑71 (MQ=255) aCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGc > 1:2327977/1‑71 (MQ=255) aCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAAGGc < 1:1573352/71‑1 (MQ=255) cGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCt < 1:1203280/71‑1 (MQ=255) cTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCa > 1:2080787/1‑63 (MQ=255) gACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTccc < 1:2819519/69‑1 (MQ=255) gCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgac > 1:3545396/1‑71 (MQ=255) ccGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCa < 1:2673428/44‑1 (MQ=255) ccGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgact > 1:3544092/1‑71 (MQ=255) ccGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgact < 1:1795505/71‑1 (MQ=255) cGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGactgactg > 1:546169/1‑71 (MQ=255) cGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGactgactg > 1:3840697/1‑71 (MQ=255) tGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCtt > 1:3829742/1‑57 (MQ=255) gAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCggg > 1:299584/1‑47 (MQ=255) gAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCggg > 1:295014/1‑47 (MQ=255) gAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTccc > 1:2759982/1‑59 (MQ=255) gCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAAc < 1:1808116/71‑1 (MQ=255) ggAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGc > 1:1609513/1‑40 (MQ=255) gAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGa < 1:979206/62‑1 (MQ=255) atatGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGaa < 1:3411636/70‑1 (MQ=255) tGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGaaaaa > 1:729834/1‑70 (MQ=255) tGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAAt < 1:2451514/71‑1 (MQ=255) gCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAg > 1:1110957/1‑63 (MQ=255) cGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATa > 1:733953/1‑70 (MQ=255) gTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATAc > 1:3680573/1‑70 (MQ=255) ggTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACa > 1:3216736/1‑69 (MQ=255) gTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAgg < 1:2884836/59‑1 (MQ=255) ttCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGactgactg < 1:2581109/47‑1 (MQ=255) acaacGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgac > 1:1671379/1‑41 (MQ=255) acaacGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAggcggc > 1:1564678/1‑71 (MQ=255) aacGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTg > 1:3576067/1‑71 (MQ=255) | CTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAAAAATACAAAGGCGGCTG > minE/835345‑835479 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |