Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 835,873 G→A 28.8% P197P (CCG→CCA narH → nitrate reductase 1, beta (Fe‑S) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE835,8730GA28.8% 85.8 / 40.7 59P197P (CCG→CCAnarHnitrate reductase 1, beta (Fe‑S) subunit
Reads supporting (aligned to +/- strand):  ref base G (23/19);  new base A (8/9);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

ACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGT  >  minE/835805‑835936
                                                                     |                                                               
acCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAg                                                               >  1:1576109/1‑71 (MQ=255)
  cTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCg                                                             <  1:3489727/71‑1 (MQ=255)
  cTTCATGATGTACCTGCCTCGCCTGTGCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCg                                                             <  1:3854788/71‑1 (MQ=255)
    tCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGt                                                           >  1:740119/1‑71 (MQ=255)
    tCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGt                                                           >  1:2237282/1‑71 (MQ=255)
     cATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCggcg                                                          >  1:1591014/1‑69 (MQ=255)
      atgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCggcg                                                          <  1:231505/70‑3 (MQ=255)
       tgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGCTGCGGGGCGACCTGCCCAAGCGgcgc                                                         <  1:2500398/70‑1 (MQ=255)
         atgTATTTGCCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGAt                                                      <  1:2439608/71‑1 (MQ=255)
         atgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCgg                                                            >  1:2599389/1‑65 (MQ=255)
         atgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCGGCGCTAt                                                      >  1:2411484/1‑71 (MQ=255)
          tgTATTTGCCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGAtt                                                     <  1:2838530/71‑1 (MQ=255)
            tATTTGCCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCg                                                             <  1:3267660/61‑1 (MQ=255)
                  ccGCGCCTGTGCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACg                                             <  1:1166262/71‑1 (MQ=255)
                  ccGCGCCTGTGCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACg                                             >  1:3538935/1‑71 (MQ=255)
                  ccGCGCCTGTGCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACg                                             >  1:1358102/1‑71 (MQ=255)
                      gcCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTg                                           >  1:2514634/1‑69 (MQ=255)
                          gtgCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaag                                        >  1:1263113/1‑68 (MQ=255)
                           tgCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa                                      <  1:2287669/69‑1 (MQ=255)
                           tgCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaag                                        >  1:2721897/1‑67 (MQ=255)
                           tgCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGaagaag                                     <  1:3641584/70‑1 (MQ=255)
                           tgCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaagaa                                      <  1:1615586/69‑1 (MQ=255)
                           tgCGAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaagaa                                      <  1:137544/69‑1 (MQ=255)
                              gAACACTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACgcg                                           <  1:999948/61‑3 (MQ=255)
                               aaCACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg                                 >  1:2532054/1‑70 (MQ=255)
                               aaCACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg                                 >  1:1490961/1‑70 (MQ=255)
                               aaCACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg                                 >  1:3889425/1‑70 (MQ=255)
                                acacTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCa                               <  1:1259087/71‑1 (MQ=255)
                                 cacTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCAt                              >  1:3385341/1‑71 (MQ=255)
                                 cacTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCAt                              >  1:3423891/1‑71 (MQ=255)
                                    tGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGt                           >  1:499022/1‑71 (MQ=255)
                                      ccTGAACCCGGCATGTGTGGCGAGCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTc                          <  1:3077938/70‑1 (MQ=255)
                                      ccTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTAc                                                  <  1:539961/46‑1 (MQ=255)
                                       cTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt                        <  1:3603997/71‑1 (MQ=255)
                                          aaCCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGa                      <  1:1373010/70‑1 (MQ=255)
                                             cccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCg                   <  1:982906/71‑1 (MQ=255)
                                               cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc                 >  1:3930504/1‑71 (MQ=255)
                                                 gCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTc                          >  1:3153570/1‑60 (MQ=255)
                                                  cATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAg              >  1:1687624/1‑71 (MQ=255)
                                                    tgtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa                                      <  1:913346/45‑1 (MQ=255)
                                                      tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAgaga           >  1:1414510/1‑67 (MQ=255)
                                                      tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt           <  1:3621278/70‑1 (MQ=255)
                                                      cgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATa          <  1:2255555/70‑1 (MQ=255)
                                                       gtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATc                    >  1:804926/1‑60 (MQ=255)
                                                        tgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga                                    <  1:562337/43‑1 (MQ=255)
                                                        tgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa        <  1:3425337/71‑1 (MQ=255)
                                                        tgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa        <  1:1143689/71‑1 (MQ=255)
                                                         ggCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAAt       >  1:1969010/1‑71 (MQ=255)
                                                         ggCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAAt       >  1:820397/1‑71 (MQ=255)
                                                          gCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATg      >  1:2882119/1‑71 (MQ=255)
                                                           cGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGc     <  1:3429905/71‑1 (MQ=255)
                                                             aCCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg   >  1:728049/1‑71 (MQ=255)
                                                             aCCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg   >  1:1984304/1‑71 (MQ=255)
                                                              ccTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGt  <  1:529350/71‑1 (MQ=255)
                                                                 gCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGa                      >  1:3035260/1‑48 (MQ=255)
                                                                 gCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGAt                     >  1:951557/1‑49 (MQ=255)
                                                                  cccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGcc    >  1:2063342/1‑65 (MQ=255)
                                                                   ccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa        >  1:354336/1‑60 (MQ=255)
                                                                    cGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATc                             <  1:2830272/38‑1 (MQ=255)
                                                                     |                                                               
ACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGT  >  minE/835805‑835936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: