| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 190,117 | T→C | 37.2% | S538S (AGT→AGC) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 190,117 | 0 | T | C | 37.2% | 40.5 / 49.2 | 51 | S538S (AGT→AGC) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (15/17); new base C (6/13); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
AAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTAC > minE/190048‑190183 | aaaaTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTa < 1:3716269/71‑1 (MQ=255) aaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAt < 1:2288050/71‑1 (MQ=255) aaTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTAt > 1:1495476/1‑70 (MQ=255) aaTACCTCGACGATCATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc > 1:3036022/1‑70 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAt < 1:1999686/70‑1 (MQ=255) aTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg < 1:3616792/71‑1 (MQ=255) cTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTAt < 1:2701062/71‑1 (MQ=255) cTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTAt < 1:2073456/71‑1 (MQ=255) cgacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATc > 1:2661880/1‑70 (MQ=255) gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCg < 1:3073030/70‑1 (MQ=255) gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGa > 1:1524719/1‑71 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:498848/70‑1 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:742748/71‑1 (MQ=255) cgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt > 1:2295930/1‑70 (MQ=255) aCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaaaa < 1:3567069/71‑1 (MQ=255) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCa < 1:1405728/71‑1 (MQ=255) ggcaTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:1101192/60‑1 (MQ=12) gcaTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaaa < 1:2785490/69‑1 (MQ=255) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:3696164/70‑1 (MQ=255) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:3691571/70‑1 (MQ=255) aTCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg > 1:39732/1‑52 (MQ=255) aTCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCaa < 1:92617/68‑1 (MQ=255) tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa > 1:2204207/1‑71 (MQ=255) tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa < 1:1021879/71‑1 (MQ=255) tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa < 1:3732692/71‑1 (MQ=255) gttgagaAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:3158362/4‑62 (MQ=255) gtagAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATgg < 1:1373617/70‑1 (MQ=255) gtagAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATgg < 1:720342/70‑1 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAAt > 1:2195073/1‑65 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg < 1:626228/40‑1 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg < 1:3842599/40‑1 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTg < 1:2744420/40‑1 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGc < 1:1483645/59‑1 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATgg < 1:3110597/64‑1 (MQ=255) ccGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGt < 1:2901634/71‑1 (MQ=255) cGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg > 1:3067743/1‑71 (MQ=255) ccTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGtt > 1:2851227/1‑71 (MQ=255) ccTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGtt > 1:2788236/1‑71 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGatta < 1:2108835/56‑1 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:1323502/1‑71 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGACTATTGCGTGGGTTGACg > 1:588582/1‑71 (MQ=255) ataACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCac > 1:1819542/1‑47 (MQ=255) ataACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCac > 1:955875/1‑47 (MQ=255) aCCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAAt > 1:3657983/1‑46 (MQ=255) ctgctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACgg < 1:1493677/67‑1 (MQ=255) tttCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCaaa < 1:2156852/69‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGct > 1:2558698/1‑71 (MQ=255) tttAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAAGGCTCtt > 1:1746693/1‑70 (MQ=255) tAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTAc > 1:220243/1‑70 (MQ=255) tAGTATTGGCATAGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTAc > 1:1208391/1‑70 (MQ=255) aGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTc < 1:1201368/57‑1 (MQ=255) | AAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTAC > minE/190048‑190183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |