| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,882 | G→T | 25.3% | A200A (GCG→GCT) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,882 | 0 | G | T | 25.3% | 136.8 / 29.8 | 63 | A200A (GCG→GCT) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (26/21); new base T (7/9); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.12e-01 | |||||||||||
ATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTG > minE/835814‑835950 | atgTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGAt < 1:2439608/71‑1 (MQ=255)atgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTAt > 1:2411484/1‑71 (MQ=255) tgTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGAtt < 1:2838530/71‑1 (MQ=255) ccGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACg > 1:3538935/1‑71 (MQ=255) ccGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACg < 1:1166262/71‑1 (MQ=255) ccGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACg > 1:1358102/1‑71 (MQ=255) gcCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTg > 1:2514634/1‑69 (MQ=255) gtgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaag > 1:1263113/1‑68 (MQ=255) tgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:2287669/69‑1 (MQ=255) tgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaag > 1:2721897/1‑67 (MQ=255) tgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGaagaag < 1:3641584/70‑1 (MQ=255) tgCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaagaa < 1:137544/69‑1 (MQ=255) tgCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaagaa < 1:1615586/69‑1 (MQ=255) gAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACgcg < 1:999948/61‑3 (MQ=255) aaCACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg > 1:2532054/1‑70 (MQ=255) aaCACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg > 1:1490961/1‑70 (MQ=255) aaCACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg > 1:3889425/1‑70 (MQ=255) acacTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCa < 1:1259087/71‑1 (MQ=255) cacTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCAt > 1:3385341/1‑71 (MQ=255) cacTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCAt > 1:3423891/1‑71 (MQ=255) tGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGt > 1:499022/1‑71 (MQ=255) ccTGAA‑CCCGGCATGTGTGGCGAGCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTc < 1:3077938/70‑1 (MQ=255) ccTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTAc < 1:539961/46‑1 (MQ=255) cTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:3603997/71‑1 (MQ=255) aa‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGa < 1:1373010/70‑1 (MQ=255) cccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCg < 1:982906/71‑1 (MQ=255) cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc > 1:3930504/1‑71 (MQ=255) gCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTc > 1:3153570/1‑60 (MQ=255) cATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAg > 1:1687624/1‑71 (MQ=255) tgtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:913346/45‑1 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAgaga > 1:1414510/1‑67 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt < 1:3621278/70‑1 (MQ=255) cgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATa < 1:2255555/70‑1 (MQ=255) gtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATc > 1:804926/1‑60 (MQ=255) tgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga < 1:562337/43‑1 (MQ=255) tgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa < 1:1143689/71‑1 (MQ=255) tgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa < 1:3425337/71‑1 (MQ=255) ggCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAAt > 1:1969010/1‑71 (MQ=255) ggCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAAt > 1:820397/1‑71 (MQ=255) gCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATg > 1:2882119/1‑71 (MQ=255) cGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGc < 1:3429905/71‑1 (MQ=255) aCCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg > 1:1984304/1‑71 (MQ=255) aCCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg > 1:728049/1‑71 (MQ=255) ccTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGt < 1:529350/71‑1 (MQ=255) gCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGa > 1:3035260/1‑48 (MQ=255) gCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGAt > 1:951557/1‑49 (MQ=255) cccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGcc > 1:2063342/1‑65 (MQ=255) ccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:354336/1‑60 (MQ=255) caaGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggctgg < 1:3365225/69‑1 (MQ=255) caaGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggctg > 1:1106065/3‑70 (MQ=255) caaGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggctg > 1:780153/3‑70 (MQ=255) cGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATc < 1:2830272/38‑1 (MQ=255) gcggcgCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGt < 1:423219/71‑1 (MQ=255) gcggcgCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGt < 1:2485156/71‑1 (MQ=255) cGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGt < 1:3323047/70‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa < 1:2532702/68‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa < 1:1792878/68‑1 (MQ=255) gCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg > 1:3399541/1‑71 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg < 1:2331074/70‑1 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg > 1:3524678/1‑70 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg > 1:2288057/1‑68 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg > 1:2190091/1‑68 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAt > 1:943696/1‑67 (MQ=255) | ATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTG > minE/835814‑835950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |