Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 835,903 T→C 12.5% D207D (GAT→GAC narH → nitrate reductase 1, beta (Fe‑S) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE835,9030TC12.5% 185.7 / 16.1 72D207D (GAT→GACnarHnitrate reductase 1, beta (Fe‑S) subunit
Reads supporting (aligned to +/- strand):  ref base T (32/31);  new base C (4/5);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

AACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAA  >  minE/835836‑835971
                                                                    |                                                                    
aaCACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg                                                                    >  1:2532054/1‑70 (MQ=255)
aaCACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg                                                                    >  1:3889425/1‑70 (MQ=255)
aaCACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg                                                                    >  1:1490961/1‑70 (MQ=255)
 acacTGCCTCAATCCCAGCTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCa                                                                  <  1:1259087/71‑1 (MQ=255)
  cacTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCAt                                                                 >  1:3385341/1‑71 (MQ=255)
  cacTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCAt                                                                 >  1:3423891/1‑71 (MQ=255)
     tGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGt                                                              >  1:499022/1‑71 (MQ=255)
       ccTGAACCCGGCATGTGTGGCGAGCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTc                                                             <  1:3077938/70‑1 (MQ=255)
        cTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt                                                           <  1:3603997/71‑1 (MQ=255)
           aaCCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGa                                                         <  1:1373010/70‑1 (MQ=255)
              cccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCg                                                      <  1:982906/71‑1 (MQ=255)
                cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc                                                    >  1:3930504/1‑71 (MQ=255)
                  gCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTc                                                             >  1:3153570/1‑60 (MQ=255)
                   cATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAg                                                 >  1:1687624/1‑71 (MQ=255)
                       tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAgaga                                              >  1:1414510/1‑67 (MQ=255)
                       tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt                                              <  1:3621278/70‑1 (MQ=255)
                       cgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATa                                             <  1:2255555/70‑1 (MQ=255)
                        gtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATc                                                       >  1:804926/1‑60 (MQ=255)
                         tgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa                                           <  1:3425337/71‑1 (MQ=255)
                         tgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa                                           <  1:1143689/71‑1 (MQ=255)
                          ggCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAAt                                          >  1:1969010/1‑71 (MQ=255)
                          ggCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAAt                                          >  1:820397/1‑71 (MQ=255)
                           gCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATg                                         >  1:2882119/1‑71 (MQ=255)
                            cGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGc                                        <  1:3429905/71‑1 (MQ=255)
                              aCCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg                                      >  1:728049/1‑71 (MQ=255)
                              aCCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg                                      >  1:1984304/1‑71 (MQ=255)
                               ccTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGt                                     <  1:529350/71‑1 (MQ=255)
                                  gCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGa                                                         >  1:3035260/1‑48 (MQ=255)
                                  gCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGAt                                                        >  1:951557/1‑49 (MQ=255)
                                   cccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGcc                                       >  1:2063342/1‑65 (MQ=255)
                                    ccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa                                           >  1:354336/1‑60 (MQ=255)
                                     caaGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggctgg                               <  1:3365225/69‑1 (MQ=255)
                                     caaGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggctg                                >  1:1106065/3‑70 (MQ=255)
                                     caaGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggctg                                >  1:780153/3‑70 (MQ=255)
                                     cGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATc                                                                <  1:2830272/38‑1 (MQ=255)
                                        gcggcgCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGt                            <  1:2485156/71‑1 (MQ=255)
                                        gcggcgCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGt                            <  1:423219/71‑1 (MQ=255)
                                         cGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGt                            <  1:3323047/70‑1 (MQ=255)
                                            tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa                           <  1:2532702/68‑1 (MQ=255)
                                            tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa                           <  1:1792878/68‑1 (MQ=255)
                                             gCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                       >  1:3399541/1‑71 (MQ=255)
                                              cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                       >  1:3524678/1‑70 (MQ=255)
                                              cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                       <  1:2331074/70‑1 (MQ=255)
                                              cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg                         >  1:2288057/1‑68 (MQ=255)
                                              cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg                         >  1:2190091/1‑68 (MQ=255)
                                              cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAt                          >  1:943696/1‑67 (MQ=255)
                                                aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt                                              <  1:1692177/45‑1 (MQ=255)
                                                aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt                    >  1:163782/1‑71 (MQ=255)
                                                 tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa                                             >  1:3559595/1‑45 (MQ=255)
                                                 tttAAAAGCGTGAAGAAGATGGCATCGTCCTGATCGCCCAGGATa                                             >  1:1291911/1‑45 (MQ=255)
                                                  ttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaa                                            >  1:3580415/1‑45 (MQ=255)
                                                  ttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa                  >  1:2083247/1‑71 (MQ=255)
                                                    actaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc                 <  1:1885904/67‑1 (MQ=255)
                                                    aCAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGt                                     <  1:2037880/50‑1 (MQ=255)
                                                     cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt                <  1:293862/70‑1 (MQ=255)
                                                        gCGTGAAGAAGATGGCATCGTCCTGATCGACCAgg                                                <  1:545146/35‑1 (MQ=255)
                                                        gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt            <  1:364089/71‑1 (MQ=255)
                                                        gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt            <  1:2115226/71‑1 (MQ=255)
                                                        gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt            <  1:2291124/71‑1 (MQ=255)
                                                        gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt            <  1:2449836/71‑1 (MQ=255)
                                                           tGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg                             <  1:1785532/51‑1 (MQ=255)
                                                            gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg               <  1:2415818/64‑1 (MQ=255)
                                                            gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg               <  1:1989575/64‑1 (MQ=255)
                                                            gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGccc        >  1:3641783/1‑71 (MQ=255)
                                                            gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGccc        >  1:1734274/1‑71 (MQ=255)
                                                             aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg       <  1:3582159/71‑1 (MQ=255)
                                                             aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg       <  1:986271/71‑1 (MQ=255)
                                                              agaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg       >  1:3266908/1‑70 (MQ=255)
                                                                aagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctgg                               <  1:1841925/44‑1 (MQ=255)
                                                                aagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg              <  1:1780459/61‑1 (MQ=255)
                                                                 agaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGa             >  1:933712/1‑61 (MQ=255)
                                                                  gaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaa  <  1:781931/71‑1 (MQ=255)
                                                                    tGGCATCGTCCTGATCGACCAGGATAAATGCCGtg                                    >  1:1358608/1‑35 (MQ=255)
                                                                    tGGCATCGTCCTGAACGACCAGGATAAATGCCGTGGCTGGCGTAtgt                        >  1:2608690/1‑47 (MQ=255)
                                                                    |                                                                    
AACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAA  >  minE/835836‑835971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: