Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 835,912 C→G 14.6% V210V (GTC→GTG narH → nitrate reductase 1, beta (Fe‑S) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE835,9120CG14.6% 182.9 / 21.1 75V210V (GTC→GTGnarHnitrate reductase 1, beta (Fe‑S) subunit
Reads supporting (aligned to +/- strand):  ref base C (30/34);  new base G (6/5);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

CCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTAC  >  minE/835843‑835981
                                                                     |                                                                     
ccTGAACCCGGCATGTGTGGCGAGCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTc                                                                       <  1:3077938/70‑1 (MQ=255)
 cTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt                                                                     <  1:3603997/71‑1 (MQ=255)
    aaCCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGa                                                                   <  1:1373010/70‑1 (MQ=255)
      cccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCg                                                                <  1:982906/71‑1 (MQ=255)
        cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc                                                              >  1:3930504/1‑71 (MQ=255)
          gCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTc                                                                       >  1:3153570/1‑60 (MQ=255)
           cATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAg                                                           >  1:1687624/1‑71 (MQ=255)
               tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAgaga                                                        >  1:1414510/1‑67 (MQ=255)
               tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt                                                        <  1:3621278/70‑1 (MQ=255)
               cgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATa                                                       <  1:2255555/70‑1 (MQ=255)
                gtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATc                                                                 >  1:804926/1‑60 (MQ=255)
                 tgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa                                                     <  1:3425337/71‑1 (MQ=255)
                 tgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa                                                     <  1:1143689/71‑1 (MQ=255)
                  ggCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAAt                                                    >  1:1969010/1‑71 (MQ=255)
                  ggCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAAt                                                    >  1:820397/1‑71 (MQ=255)
                   gCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATg                                                   >  1:2882119/1‑71 (MQ=255)
                    cGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGc                                                  <  1:3429905/71‑1 (MQ=255)
                      aCCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg                                                >  1:728049/1‑71 (MQ=255)
                      aCCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg                                                >  1:1984304/1‑71 (MQ=255)
                       ccTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGt                                               <  1:529350/71‑1 (MQ=255)
                          gCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGa                                                                   >  1:3035260/1‑48 (MQ=255)
                          gCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGAt                                                                  >  1:951557/1‑49 (MQ=255)
                           cccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGcc                                                 >  1:2063342/1‑65 (MQ=255)
                            ccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa                                                     >  1:354336/1‑60 (MQ=255)
                             caaGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggctgg                                         <  1:3365225/69‑1 (MQ=255)
                             caaGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggctg                                          >  1:780153/3‑70 (MQ=255)
                             caaGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggctg                                          >  1:1106065/3‑70 (MQ=255)
                                gcggcgCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGt                                      <  1:423219/71‑1 (MQ=255)
                                gcggcgCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGt                                      <  1:2485156/71‑1 (MQ=255)
                                 cGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGt                                      <  1:3323047/70‑1 (MQ=255)
                                    tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa                                     <  1:1792878/68‑1 (MQ=255)
                                    tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa                                     <  1:2532702/68‑1 (MQ=255)
                                     gCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                                 >  1:3399541/1‑71 (MQ=255)
                                      cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                                 <  1:2331074/70‑1 (MQ=255)
                                      cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                                 >  1:3524678/1‑70 (MQ=255)
                                      cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg                                   >  1:2190091/1‑68 (MQ=255)
                                      cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg                                   >  1:2288057/1‑68 (MQ=255)
                                      cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAt                                    >  1:943696/1‑67 (MQ=255)
                                        aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt                                                        <  1:1692177/45‑1 (MQ=255)
                                        aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt                              >  1:163782/1‑71 (MQ=255)
                                         tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa                                                       >  1:3559595/1‑45 (MQ=255)
                                         tttAAAAGCGTGAAGAAGATGGCATCGTCCTGATCGCCCAGGATa                                                       >  1:1291911/1‑45 (MQ=255)
                                          ttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaa                                                      >  1:3580415/1‑45 (MQ=255)
                                          ttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa                            >  1:2083247/1‑71 (MQ=255)
                                            actaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc                           <  1:1885904/67‑1 (MQ=255)
                                            aCAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGt                                               <  1:2037880/50‑1 (MQ=255)
                                             cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt                          <  1:293862/70‑1 (MQ=255)
                                                gCGTGAAGAAGATGGCATCGTCCTGATCGACCAgg                                                          <  1:545146/35‑1 (MQ=255)
                                                gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt                      <  1:2449836/71‑1 (MQ=255)
                                                gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt                      <  1:2291124/71‑1 (MQ=255)
                                                gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt                      <  1:364089/71‑1 (MQ=255)
                                                gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt                      <  1:2115226/71‑1 (MQ=255)
                                                   tGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg                                       <  1:1785532/51‑1 (MQ=255)
                                                    gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg                         <  1:2415818/64‑1 (MQ=255)
                                                    gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg                         <  1:1989575/64‑1 (MQ=255)
                                                    gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGccc                  >  1:1734274/1‑71 (MQ=255)
                                                    gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGccc                  >  1:3641783/1‑71 (MQ=255)
                                                     aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg                 <  1:986271/71‑1 (MQ=255)
                                                     aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg                 <  1:3582159/71‑1 (MQ=255)
                                                      agaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg                 >  1:3266908/1‑70 (MQ=255)
                                                        aagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctgg                                         <  1:1841925/44‑1 (MQ=255)
                                                        aagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg                        <  1:1780459/61‑1 (MQ=255)
                                                         agaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGa                       >  1:933712/1‑61 (MQ=255)
                                                          gacggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACa             >  1:2196522/4‑70 (MQ=255)
                                                          gaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaa            <  1:781931/71‑1 (MQ=255)
                                                            tGGCATCGTCCTGATCGACCAGGATAAATGCCGtg                                              >  1:1358608/1‑35 (MQ=255)
                                                            tGGCATCGTCCTGAACGACCAGGATAAATGCCGTGGCTGGCGTAtgt                                  >  1:2608690/1‑47 (MQ=255)
                                                            cggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaa           >  1:155852/2‑70 (MQ=255)
                                                             ggCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaa          >  1:1867789/1‑70 (MQ=255)
                                                              gCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaaaa        <  1:71648/71‑1 (MQ=255)
                                                              gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaa        <  1:635731/71‑1 (MQ=255)
                                                              gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaa        <  1:3279261/71‑1 (MQ=255)
                                                               cATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAt                                    >  1:3091753/1‑42 (MQ=255)
                                                               cATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaaa       <  1:177164/71‑1 (MQ=255)
                                                                 tCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc     >  1:3691146/1‑71 (MQ=255)
                                                                   gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCt    <  1:2782806/70‑1 (MQ=255)
                                                                    tCCTGATCGACCAGGATAAATGCCGTGGCTGGCTTATGTGCATCACTGGATGCCCGTACAAAAAAATCTAc  <  1:925842/71‑1 (MQ=255)
                                                                     |                                                                     
CCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTAC  >  minE/835843‑835981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: