Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 835,946 A→T 22.4% M222L (ATG→TTG)  narH → nitrate reductase 1, beta (Fe‑S) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE835,9460AT22.4% 135.0 / 37.5 67M222L (ATG→TTG) narHnitrate reductase 1, beta (Fe‑S) subunit
Reads supporting (aligned to +/- strand):  ref base A (23/29);  new base T (6/9);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAT  >  minE/835879‑836016
                                                                   |                                                                      
tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa                                                                        <  1:2532702/68‑1 (MQ=255)
tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa                                                                        <  1:1792878/68‑1 (MQ=255)
 gCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                                                                    >  1:3399541/1‑71 (MQ=255)
  cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                                                                    <  1:2331074/70‑1 (MQ=255)
  cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                                                                    >  1:3524678/1‑70 (MQ=255)
  cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg                                                                      >  1:2288057/1‑68 (MQ=255)
  cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg                                                                      >  1:2190091/1‑68 (MQ=255)
  cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAt                                                                       >  1:943696/1‑67 (MQ=255)
    aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt                                                                 >  1:163782/1‑71 (MQ=255)
      ttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa                                                               >  1:2083247/1‑71 (MQ=255)
        actaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc                                                              <  1:1885904/67‑1 (MQ=255)
         cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt                                                             <  1:293862/70‑1 (MQ=255)
            gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt                                                         <  1:364089/71‑1 (MQ=255)
            gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt                                                         <  1:2291124/71‑1 (MQ=255)
            gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt                                                         <  1:2449836/71‑1 (MQ=255)
            gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt                                                         <  1:2115226/71‑1 (MQ=255)
                gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg                                                            <  1:2415818/64‑1 (MQ=255)
                gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg                                                            <  1:1989575/64‑1 (MQ=255)
                gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGccc                                                     >  1:3641783/1‑71 (MQ=255)
                gaagaaGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGccc                                                     >  1:1734274/1‑71 (MQ=255)
                 aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg                                                    <  1:986271/71‑1 (MQ=255)
                 aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg                                                    <  1:3582159/71‑1 (MQ=255)
                  agaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg                                                    >  1:3266908/1‑70 (MQ=255)
                    aagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg                                                           <  1:1780459/61‑1 (MQ=255)
                     agaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGa                                                          >  1:933712/1‑61 (MQ=255)
                      gacggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACa                                                >  1:2196522/4‑70 (MQ=255)
                      gaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaa                                               <  1:781931/71‑1 (MQ=255)
                        tGGCATCGTCCTGAACGACCAGGATAAATGCCGTGGCTGGCGTAtgt                                                                     >  1:2608690/1‑47 (MQ=255)
                        cggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaa                                              >  1:155852/2‑70 (MQ=255)
                         ggCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaa                                             >  1:1867789/1‑70 (MQ=255)
                          gCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaaaa                                           <  1:71648/71‑1 (MQ=255)
                          gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaa                                           <  1:635731/71‑1 (MQ=255)
                          gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaa                                           <  1:3279261/71‑1 (MQ=255)
                           cATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAt                                                                       >  1:3091753/1‑42 (MQ=255)
                           cATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaaa                                          <  1:177164/71‑1 (MQ=255)
                             tCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc                                        >  1:3691146/1‑71 (MQ=255)
                               gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCt                                       <  1:2782806/70‑1 (MQ=255)
                                tCCTGATCGACCAGGATAAATGCCGTGGCTGGCTTATGTGCATCACTGGATGCCCGTACAAAAAAATCTAc                                     <  1:925842/71‑1 (MQ=255)
                                  cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACt                                    <  1:219070/70‑1 (MQ=255)
                                  cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACt                                    <  1:100622/70‑1 (MQ=255)
                                   tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACtt                                   >  1:2159777/1‑70 (MQ=255)
                                    gATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGccc                                                     >  1:256887/1‑51 (MQ=255)
                                        gACCAGGATAAATGCCGTGGGTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTg                             <  1:2925096/71‑1 (MQ=255)
                                          tcAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGa                           <  1:3108239/70‑1 (MQ=255)
                                              gATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAaaag                       >  1:3892619/1‑71 (MQ=255)
                                              gATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAagag                       >  1:3515872/1‑71 (MQ=255)
                                                tAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCg                     <  1:271886/71‑1 (MQ=255)
                                                tAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCg                     <  1:3585669/71‑1 (MQ=255)
                                                   aTGCCGTGGCTGGCGTATGTGCATCACTGGATGccc                                                     >  1:2835075/1‑36 (MQ=255)
                                                   aTGCCGTGGCTGGCGTATGTGCATCACTGGATGccc                                                     >  1:1655903/1‑36 (MQ=255)
                                                    tGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCaa                 <  1:838168/71‑1 (MQ=255)
                                                      ccGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGt               <  1:2965818/71‑1 (MQ=255)
                                                      ccGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGt               <  1:604585/71‑1 (MQ=255)
                                                        gTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCt             <  1:239832/71‑1 (MQ=255)
                                                         tggctggcGTATGTGCATCTCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGaa                          <  1:3299187/57‑1 (MQ=255)
                                                         tggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTg            <  1:2128510/71‑1 (MQ=255)
                                                          ggctggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTcag            >  1:1195984/1‑68 (MQ=255)
                                                          ggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCt             >  1:2222021/1‑69 (MQ=255)
                                                           gctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgag          <  1:203394/71‑1 (MQ=255)
                                                             tggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAgaaa         >  1:1152407/1‑68 (MQ=255)
                                                               gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaaat      >  1:1719179/1‑69 (MQ=255)
                                                               gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa        <  1:3241472/69‑1 (MQ=255)
                                                               gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa        <  1:1843920/69‑1 (MQ=255)
                                                               gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa        <  1:3246154/69‑1 (MQ=255)
                                                               gcGTATGTTCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg       >  1:2915481/1‑70 (MQ=255)
                                                               gcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGt      >  1:3747510/1‑71 (MQ=255)
                                                                cGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgag          <  1:934189/66‑1 (MQ=255)
                                                                  tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCa   <  1:1710470/71‑1 (MQ=255)
                                                                  tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCa   <  1:1503231/71‑1 (MQ=255)
                                                                   aTGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt  >  1:1890133/1‑71 (MQ=255)
                                                                   aTGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt  >  1:309516/1‑71 (MQ=255)
                                                                   |                                                                      
TGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAT  >  minE/835879‑836016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: