| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,993 | G→A | 25.0% | K237K (AAG→AAA) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,993 | 0 | G | A | 25.0% | 114.5 / 36.0 | 64 | K237K (AAG→AAA) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (14/34); new base A (5/11); total (19/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAG > minE/835925‑836063 | gATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAaaag > 1:3892619/1‑71 (MQ=255)gATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAagag > 1:3515872/1‑71 (MQ=255) tAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCg < 1:271886/71‑1 (MQ=255) tAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCg < 1:3585669/71‑1 (MQ=255) tGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCaa < 1:838168/71‑1 (MQ=255) ccGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGt < 1:2965818/71‑1 (MQ=255) ccGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGt < 1:604585/71‑1 (MQ=255) gTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCt < 1:239832/71‑1 (MQ=255) tggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTg < 1:2128510/71‑1 (MQ=255) ggctggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTcag > 1:1195984/1‑68 (MQ=255) ggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCt > 1:2222021/1‑69 (MQ=255) gctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgag < 1:203394/71‑1 (MQ=255) tggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAgaaa > 1:1152407/1‑68 (MQ=255) gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaaat > 1:1719179/1‑69 (MQ=255) gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa < 1:3241472/69‑1 (MQ=255) gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa < 1:1843920/69‑1 (MQ=255) gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa < 1:3246154/69‑1 (MQ=255) gcGTATGTTCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg > 1:2915481/1‑70 (MQ=255) gcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGt > 1:3747510/1‑71 (MQ=255) cGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgag < 1:934189/66‑1 (MQ=255) tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCa < 1:1503231/71‑1 (MQ=255) tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCa < 1:1710470/71‑1 (MQ=255) aTGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:1890133/1‑71 (MQ=255) aTGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:309516/1‑71 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtc < 1:1561817/71‑1 (MQ=255) tgtgCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCAtc > 1:778851/1‑71 (MQ=255) cATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGt > 1:207690/1‑53 (MQ=255) cATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtcttc < 1:1723363/70‑1 (MQ=255) tCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGc > 1:241643/1‑71 (MQ=255) aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa < 1:501911/71‑1 (MQ=255) aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa < 1:2942732/71‑1 (MQ=255) cTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTAt > 1:3086932/1‑71 (MQ=255) ggATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATcc > 1:2096601/1‑71 (MQ=255) gATGCCCGTACAAAAAAATCTACTTCAATTGGAAGAGCGGTAAGTc < 1:2849429/46‑1 (MQ=255) gATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTc < 1:583075/46‑1 (MQ=255) aTGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGaa < 1:2546401/51‑1 (MQ=255) tGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTga < 1:136854/47‑1 (MQ=255) tccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacgaa < 1:2509527/70‑3 (MQ=255) cGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTAtt < 1:1497057/71‑1 (MQ=255) gTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTg < 1:960377/71‑1 (MQ=255) tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgaga < 1:1231986/43‑1 (MQ=255) cAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:2328939/1‑48 (MQ=255) cAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGag < 1:1155359/70‑2 (MQ=255) aaaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtcttc < 1:3307498/50‑1 (MQ=255) aaaaaaTCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaaat < 1:2405111/42‑3 (MQ=255) aaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTa < 1:3062759/62‑1 (MQ=255) aaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTg < 1:1252982/65‑1 (MQ=255) aaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCCTCTGCTATCCGCGTAtt < 1:299188/64‑1 (MQ=255) aaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTATGCTATCCGCGTATTGAAGCggg < 1:3819574/71‑1 (MQ=255) aTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCa < 1:3498115/71‑1 (MQ=255) tCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCggg < 1:3554602/67‑1 (MQ=255) tCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCggg < 1:2545390/67‑1 (MQ=255) tCTACTTCAACTGGAAGAGAGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAg > 1:3360921/1‑63 (MQ=255) aCTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtctt < 1:1433275/40‑1 (MQ=255) cTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGcc < 1:2234600/69‑1 (MQ=255) cTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGcc < 1:3729428/69‑1 (MQ=255) cTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCg < 1:1050029/70‑1 (MQ=255) cTTCAACTCCAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAACCGGGTCAGcc < 1:224040/69‑1 (MQ=255) cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg > 1:1174327/1‑71 (MQ=255) cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg > 1:2590286/1‑71 (MQ=255) cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg > 1:39260/1‑71 (MQ=255) aaCTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCgt < 1:514625/71‑1 (MQ=255) aaCTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCgt < 1:1229407/71‑1 (MQ=255) aCTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTac < 1:611707/41‑2 (MQ=12) tGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGtgtg > 1:2997512/1‑71 (MQ=255) gAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCt < 1:534380/71‑1 (MQ=255) gagCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:364190/45‑1 (MQ=255) gagCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:359474/45‑1 (MQ=255) gagCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAg < 1:431094/71‑1 (MQ=255) | GATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAG > minE/835925‑836063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |