| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,008 | G→A | 17.2% | E242E (GAG→GAA) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,008 | 0 | G | A | 17.2% | 149.5 / 22.0 | 64 | E242E (GAG→GAA) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (19/34); new base A (2/9); total (21/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGT > minE/835938‑836077 | gctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgag < 1:203394/71‑1 (MQ=255) gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaaat > 1:1719179/1‑69 (MQ=255) gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa < 1:3246154/69‑1 (MQ=255) gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa < 1:1843920/69‑1 (MQ=255) gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa < 1:3241472/69‑1 (MQ=255) gcGTATGTTCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg > 1:2915481/1‑70 (MQ=255) gcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGt > 1:3747510/1‑71 (MQ=255) cGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgag < 1:934189/66‑1 (MQ=255) tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCa < 1:1503231/71‑1 (MQ=255) tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCa < 1:1710470/71‑1 (MQ=255) aTGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:309516/1‑71 (MQ=255) aTGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:1890133/1‑71 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtc < 1:1561817/71‑1 (MQ=255) tgtgCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCAtc > 1:778851/1‑71 (MQ=255) cATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtcttc < 1:1723363/70‑1 (MQ=255) tCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGc > 1:241643/1‑71 (MQ=255) aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa < 1:501911/71‑1 (MQ=255) aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa < 1:2942732/71‑1 (MQ=255) cTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTAt > 1:3086932/1‑71 (MQ=255) ggATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATcc > 1:2096601/1‑71 (MQ=255) aTGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGaa < 1:2546401/51‑1 (MQ=255) tccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacgaa < 1:2509527/70‑3 (MQ=255) cGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTAtt < 1:1497057/71‑1 (MQ=255) gTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTg < 1:960377/71‑1 (MQ=255) tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgaga < 1:1231986/43‑1 (MQ=255) cAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:2328939/1‑48 (MQ=255) cAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGag < 1:1155359/70‑2 (MQ=255) aaaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtcttc < 1:3307498/50‑1 (MQ=255) aaaaaaTCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaaat < 1:2405111/42‑3 (MQ=255) aaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTa < 1:3062759/62‑1 (MQ=255) aaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTg < 1:1252982/65‑1 (MQ=255) aaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCCTCTGCTATCCGCGTAtt < 1:299188/64‑1 (MQ=255) aaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTATGCTATCCGCGTATTGAAGCggg < 1:3819574/71‑1 (MQ=255) aTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCa < 1:3498115/71‑1 (MQ=255) tCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCggg < 1:2545390/67‑1 (MQ=255) tCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCggg < 1:3554602/67‑1 (MQ=255) tCTACTTCAACTGGAAGAGAGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAg > 1:3360921/1‑63 (MQ=255) aCTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtctt < 1:1433275/40‑1 (MQ=255) cTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGcc < 1:3729428/69‑1 (MQ=255) cTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGcc < 1:2234600/69‑1 (MQ=255) cTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCg < 1:1050029/70‑1 (MQ=255) cTTCAACTCCAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAACCGGGTCAGcc < 1:224040/69‑1 (MQ=255) cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg > 1:39260/1‑71 (MQ=255) cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg > 1:2590286/1‑71 (MQ=255) cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg > 1:1174327/1‑71 (MQ=255) aaCTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCgt < 1:1229407/71‑1 (MQ=255) aaCTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCgt < 1:514625/71‑1 (MQ=255) aCTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTac < 1:611707/41‑2 (MQ=12) tGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGtgtg > 1:2997512/1‑71 (MQ=255) gAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCt < 1:534380/71‑1 (MQ=255) gagCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:359474/45‑1 (MQ=255) gagCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:364190/45‑1 (MQ=255) gagCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAg < 1:431094/71‑1 (MQ=255) agCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAg > 1:2151562/1‑70 (MQ=255) agCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGa < 1:78065/71‑1 (MQ=255) gCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCgg < 1:1834760/48‑1 (MQ=255) gCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGa < 1:2602887/70‑1 (MQ=255) cGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCgtgt < 1:2625560/62‑1 (MQ=255) caaGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTc < 1:1988407/62‑1 (MQ=255) aaGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCgg > 1:1747970/1‑43 (MQ=255) aaGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCgg > 1:2661141/1‑43 (MQ=255) aaGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCt < 1:730330/70‑1 (MQ=255) aaGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCtg < 1:1467286/71‑1 (MQ=255) aGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCtgt > 1:1183589/1‑71 (MQ=255) aGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCtgc < 1:1451602/71‑2 (MQ=255) aGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCtgc < 1:57487/71‑2 (MQ=255) aGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCtgc < 1:1001021/71‑2 (MQ=255) gTCTGAGAAGTTCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCtgtg < 1:3809722/71‑1 (MQ=255) tCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCtgtg < 1:3616713/70‑1 (MQ=255) cTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtc > 1:1855850/1‑71 (MQ=255) cTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtc > 1:2418464/1‑71 (MQ=255) tGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtcg > 1:713415/1‑71 (MQ=255) agaAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtctgt < 1:3299085/71‑1 (MQ=255) | GCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGT > minE/835938‑836077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |