| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,317 | G→T | 32.0% | P345P (CCG→CCT) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,317 | 0 | G | T | 32.0% | 91.1 / 34.2 | 53 | P345P (CCG→CCT) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (20/16); new base T (12/5); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
AAGCCGCACAGCAGTCGCCGGTTTATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCG > minE/836250‑836386 | aaGCCGCACAGCAGTCGCCGGTTTATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGaa > 1:45822/1‑71 (MQ=255) ccGCACAGCAGTCGCCGGTTTATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCAGGAatat < 1:2303493/71‑1 (MQ=255) gCACAGCAGTCGCCGGTTTGTAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCg < 1:3517377/71‑1 (MQ=255) acaGCAGTCGCCGGTTTATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGca > 1:3843525/1‑71 (MQ=255) acaGCAGTCGCCGGTTTATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGca > 1:3552912/1‑71 (MQ=255) acaGCAGTCGCCGGTTTATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGca > 1:2114912/1‑71 (MQ=255) tCGCCGGTTTATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGcc < 1:177900/71‑1 (MQ=255) gCCGGTTTATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCg < 1:1710379/70‑1 (MQ=255) ggTTTATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCTGAATATCGCacac > 1:1663398/1‑62 (MQ=255) tttATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTc < 1:2415971/71‑1 (MQ=255) tataAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTg > 1:1321571/1‑71 (MQ=255) tacaaaaTGGCGATGGACTGGAAACTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTg > 1:1724754/4‑71 (MQ=255) taAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGt < 1:3414898/66‑1 (MQ=255) caaaaTGGCGATGGACTGGAAACTGGCACTACCGTTGCACCCTGaa > 1:2648507/2‑46 (MQ=12) caaaaTGGCGATGGACTGGAAACTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCt < 1:3323974/67‑1 (MQ=255) aaaaTGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCt < 1:760994/67‑1 (MQ=255) aaaTGGCGATGGACTGGAAACTGGCACTACCGTTGCACCCTGaa > 1:768524/1‑44 (MQ=12) aaaTGGCGATGGACTGGAAACTGGCACTACCGTTGCACCCTGaa > 1:648273/1‑44 (MQ=12) aTGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGt < 1:3284045/71‑1 (MQ=255) gatggactggaAACTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTtcct < 1:2973165/69‑2 (MQ=255) aatggaatggAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGAttg < 1:1455029/56‑3 (MQ=255) atggaatggaAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGcc > 1:294193/1‑50 (MQ=255) tggaatggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGcc > 1:3773178/1‑49 (MQ=255) tggaatggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCct < 1:1179151/71‑1 (MQ=255) tggaatggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCct > 1:2348671/1‑71 (MQ=255) ggactggaaACTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATgg < 1:124990/53‑1 (MQ=16) gaatggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCctct > 1:1978771/1‑71 (MQ=255) aatggaaACTGGCGCTGCCGCTGCATCCGGAATATc > 1:929345/1‑36 (MQ=255) aatggaaACTGGCGCTGCCGCTGCATCCGGAATATc > 1:1488579/1‑36 (MQ=255) aatggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTg > 1:702912/1‑55 (MQ=255) gaaACTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGtcac > 1:1016794/1‑69 (MQ=255) gaaACTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGtcac > 1:2646398/1‑69 (MQ=255) aaaCTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGtcac < 1:351759/70‑3 (MQ=255) aaaCTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCAcc < 1:1189360/71‑1 (MQ=255) aaCTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTcc > 1:2183290/1‑70 (MQ=255) aaCTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCg < 1:468770/71‑1 (MQ=255) aaCTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTTCGTTCCTCCGCTGTCAcc > 1:1117825/1‑70 (MQ=255) aaCTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCAcc > 1:2949734/1‑70 (MQ=255) aaCTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCAcc > 1:193198/1‑70 (MQ=255) cTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTg > 1:605674/1‑47 (MQ=255) cTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTg > 1:1158093/1‑47 (MQ=255) cTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGAt > 1:1096156/1‑71 (MQ=255) cTGGCACTACCGTTGCACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGAt > 1:2563832/1‑71 (MQ=255) gctctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCa < 1:1037688/68‑1 (MQ=255) gcgcTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCa < 1:938035/71‑1 (MQ=255) ctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTc < 1:2020396/71‑1 (MQ=255) taccgttgcACCCTGAATACCGCACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTcc > 1:2225747/3‑70 (MQ=255) ccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGAtt > 1:3817749/1‑63 (MQ=255) cATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTgcagca < 1:2173391/69‑1 (MQ=255) ccGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAcgc > 1:3561121/1‑71 (MQ=255) ccGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAcgc > 1:2260819/1‑71 (MQ=255) cGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAcgcg > 1:1220806/1‑71 (MQ=255) cGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGAcgc < 1:1796500/70‑1 (MQ=255) | AAGCCGCACAGCAGTCGCCGGTTTATAAAATGGCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCG > minE/836250‑836386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |