Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 836,459 C→A 27.6% L393M (CTG→ATG)  narH → nitrate reductase 1, beta (Fe‑S) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE836,4590CA27.6% 95.6 / 23.4 47L393M (CTG→ATG) narHnitrate reductase 1, beta (Fe‑S) subunit
Reads supporting (aligned to +/- strand):  ref base C (19/15);  new base A (8/5);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

CTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGAT  >  minE/836393‑836517
                                                                  |                                                          
cTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATctgct                                                        <  1:976080/71‑1 (MQ=255)
    gTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAc                                                     >  1:1452578/1‑70 (MQ=255)
     tAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccg                                                   >  1:1734793/1‑71 (MQ=255)
          aCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt                                              <  1:1073971/71‑1 (MQ=255)
           cGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGCCCGCCgg                                               >  1:2609738/1‑69 (MQ=255)
           cGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt                                              >  1:2610465/1‑70 (MQ=255)
           cGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt                                              >  1:641943/1‑70 (MQ=255)
           cGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt                                              >  1:3186378/1‑70 (MQ=255)
             gCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGAt                                           <  1:2401520/71‑1 (MQ=255)
                tgcTGCCCGCCATCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATAcc                                        >  1:3664896/3‑71 (MQ=255)
                  cTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCa                                       <  1:1266547/70‑1 (MQ=255)
                     ccAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCCGTGATACCAAAc                                    <  1:2405206/70‑1 (MQ=255)
                      cAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCg                                  <  1:426982/71‑1 (MQ=255)
                       aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATct                                                           <  1:1088336/45‑1 (MQ=255)
                       aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg                                 <  1:2976921/71‑1 (MQ=255)
                        gACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccgc                                                  <  1:2530743/53‑1 (MQ=255)
                        gACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccg                                                   >  1:544649/1‑52 (MQ=255)
                           gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt                             <  1:3147642/71‑1 (MQ=255)
                           atCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTa                               >  1:2848576/2‑69 (MQ=255)
                            tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGa                                                      >  1:896972/1‑45 (MQ=16)
                            tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACt                             <  1:1755072/70‑1 (MQ=255)
                             cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc                           >  1:842499/1‑71 (MQ=255)
                              gAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTgc                           <  1:2414884/70‑1 (MQ=255)
                                 aGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGa                                            >  1:236314/1‑50 (MQ=255)
                                 aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg                        >  1:2001257/1‑70 (MQ=255)
                                   ccTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTgcgggcgc                     <  1:711000/71‑3 (MQ=255)
                                      gCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTg                   >  1:68702/1‑70 (MQ=255)
                                      gCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg                                 >  1:2983601/1‑56 (MQ=255)
                                        gTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTgcggg                        >  1:23711/1‑61 (MQ=255)
                                          aTTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACg              >  1:721657/1‑71 (MQ=255)
                                           ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg              <  1:2141125/70‑1 (MQ=255)
                                           ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg              <  1:3651336/70‑1 (MQ=255)
                                           ttCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACg              <  1:2835664/70‑1 (MQ=255)
                                            tCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa            >  1:518274/1‑71 (MQ=255)
                                             ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt           >  1:3076874/1‑71 (MQ=255)
                                               ggTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGc         >  1:123649/1‑71 (MQ=255)
                                                gTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt        <  1:2599427/71‑1 (MQ=255)
                                                  aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTgg      >  1:3529363/1‑71 (MQ=255)
                                                  aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTgg      >  1:3192528/1‑71 (MQ=255)
                                                  aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTgg      >  1:721740/1‑71 (MQ=255)
                                                   cAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGc     >  1:1492931/1‑71 (MQ=255)
                                                   cAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGc     <  1:1249856/71‑1 (MQ=255)
                                                    aGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCg    <  1:1119820/71‑1 (MQ=255)
                                                      tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa                      <  1:1286605/51‑1 (MQ=255)
                                                      tATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGAt  >  1:140326/1‑71 (MQ=255)
                                                      tATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGAt  >  1:263630/1‑71 (MQ=255)
                                                       aTCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGt                                <  1:1456546/40‑1 (MQ=255)
                                                        tCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGc         <  1:190613/62‑1 (MQ=255)
                                                          tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa                      >  1:535422/1‑47 (MQ=255)
                                                                  |                                                          
CTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGAT  >  minE/836393‑836517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: