| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,462 | C→T | 27.6% | L394L (CTG→TTG) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,462 | 0 | C | T | 27.6% | 73.4 / 28.7 | 47 | L394L (CTG→TTG) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (19/15); new base T (8/5); total (27/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACG > minE/836393‑836532 | cTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATctgct < 1:976080/71‑1 (MQ=255) gTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAc > 1:1452578/1‑70 (MQ=255) tAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccg > 1:1734793/1‑71 (MQ=255) aCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt < 1:1073971/71‑1 (MQ=255) cGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGCCCGCCgg > 1:2609738/1‑69 (MQ=255) cGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt > 1:641943/1‑70 (MQ=255) cGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt > 1:3186378/1‑70 (MQ=255) cGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt > 1:2610465/1‑70 (MQ=255) gCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGAt < 1:2401520/71‑1 (MQ=255) tgcTGCCCGCCATCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATAcc > 1:3664896/3‑71 (MQ=255) cTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCa < 1:1266547/70‑1 (MQ=255) ccAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCCGTGATACCAAAc < 1:2405206/70‑1 (MQ=255) cAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCg < 1:426982/71‑1 (MQ=255) aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg < 1:2976921/71‑1 (MQ=255) gACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccgc < 1:2530743/53‑1 (MQ=255) gACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccg > 1:544649/1‑52 (MQ=255) gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt < 1:3147642/71‑1 (MQ=255) atCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTa > 1:2848576/2‑69 (MQ=255) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGa > 1:896972/1‑45 (MQ=16) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACt < 1:1755072/70‑1 (MQ=255) cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc > 1:842499/1‑71 (MQ=255) gAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTgc < 1:2414884/70‑1 (MQ=255) aGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGa > 1:236314/1‑50 (MQ=255) aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg > 1:2001257/1‑70 (MQ=255) ccTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTgcgggcgc < 1:711000/71‑3 (MQ=255) gCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTg > 1:68702/1‑70 (MQ=255) gCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg > 1:2983601/1‑56 (MQ=255) gTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTgcggg > 1:23711/1‑61 (MQ=255) aTTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACg > 1:721657/1‑71 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg < 1:2141125/70‑1 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg < 1:3651336/70‑1 (MQ=255) ttCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACg < 1:2835664/70‑1 (MQ=255) tCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:518274/1‑71 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt > 1:3076874/1‑71 (MQ=255) ggTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGc > 1:123649/1‑71 (MQ=255) gTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:2599427/71‑1 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTgg > 1:3192528/1‑71 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTgg > 1:3529363/1‑71 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTgg > 1:721740/1‑71 (MQ=255) cAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGc > 1:1492931/1‑71 (MQ=255) cAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGc < 1:1249856/71‑1 (MQ=255) aGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCg < 1:1119820/71‑1 (MQ=255) tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa < 1:1286605/51‑1 (MQ=255) tATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGAt > 1:263630/1‑71 (MQ=255) tATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGAt > 1:140326/1‑71 (MQ=255) aTCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGt < 1:1456546/40‑1 (MQ=255) tCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGc < 1:190613/62‑1 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:535422/1‑47 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACaaa > 1:3777084/1‑71 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACg > 1:1953207/1‑71 (MQ=255) | CTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACG > minE/836393‑836532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |