| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,481 | A→G | 16.1% | K400R (AAA→AGA) ‡ | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,481 | 0 | A | G | 16.1% | 140.3 / 19.9 | 62 | K400R (AAA→AGA) ‡ | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base A (27/25); new base G (5/5); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
CCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGG > minE/836414‑836550 | ccAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCCGTGATACCAAAc < 1:2405206/70‑1 (MQ=255) cAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCg < 1:426982/71‑1 (MQ=255) aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg < 1:2976921/71‑1 (MQ=255) gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt < 1:3147642/71‑1 (MQ=255) atCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTa > 1:2848576/2‑69 (MQ=255) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACt < 1:1755072/70‑1 (MQ=255) cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc > 1:842499/1‑71 (MQ=255) gAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTgc < 1:2414884/70‑1 (MQ=255) aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg > 1:2001257/1‑70 (MQ=255) ccTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTgcgggcgc < 1:711000/71‑3 (MQ=255) gCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTg > 1:68702/1‑70 (MQ=255) gCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg > 1:2983601/1‑56 (MQ=255) gTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTgcggg > 1:23711/1‑61 (MQ=255) aTTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACg > 1:721657/1‑71 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg < 1:3651336/70‑1 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg < 1:2141125/70‑1 (MQ=255) ttCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACg < 1:2835664/70‑1 (MQ=255) tCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:518274/1‑71 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt > 1:3076874/1‑71 (MQ=255) ggTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGc > 1:123649/1‑71 (MQ=255) gTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:2599427/71‑1 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTgg > 1:3192528/1‑71 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTgg > 1:721740/1‑71 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTgg > 1:3529363/1‑71 (MQ=255) cAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGc > 1:1492931/1‑71 (MQ=255) cAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGc < 1:1249856/71‑1 (MQ=255) aGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCg < 1:1119820/71‑1 (MQ=255) tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa < 1:1286605/51‑1 (MQ=255) tATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGAt > 1:140326/1‑71 (MQ=255) tATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGAt > 1:263630/1‑71 (MQ=255) aTCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGt < 1:1456546/40‑1 (MQ=255) tCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGc < 1:190613/62‑1 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:535422/1‑47 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACaaa > 1:3777084/1‑71 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACg > 1:1953207/1‑71 (MQ=255) tgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAAc > 1:1255969/1‑69 (MQ=255) gACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACg < 1:3094015/42‑1 (MQ=255) aCCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTg < 1:862594/70‑1 (MQ=255) aCCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGc < 1:351318/71‑1 (MQ=255) ccgccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:2808009/1‑42 (MQ=255) cgccgGTGATACCAAACCGGTACTGCGCGCACTGAAAc > 1:1782418/1‑38 (MQ=255) gccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGa < 1:3328494/71‑1 (MQ=255) ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGa > 1:1654898/1‑70 (MQ=255) ggTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAAc > 1:3136597/1‑71 (MQ=255) ggTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAAc > 1:2637478/1‑71 (MQ=255) aTACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:3222740/71‑1 (MQ=255) aTACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:2858762/71‑1 (MQ=255) aTACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:663707/71‑1 (MQ=255) aTACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:2773572/71‑1 (MQ=255) aTACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:3892482/71‑1 (MQ=255) aTACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:2069211/71‑1 (MQ=255) tACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGt < 1:2990046/58‑1 (MQ=255) aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGAt > 1:675893/1‑41 (MQ=255) aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGAt > 1:1998264/1‑41 (MQ=255) aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCg < 1:2118596/67‑1 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACg > 1:2734011/1‑55 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACg > 1:2390871/1‑55 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa < 1:3570913/70‑1 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACCTATGCTGGc < 1:1992818/37‑1 (MQ=255) cAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACg > 1:1338379/1‑71 (MQ=255) cAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACg > 1:1592283/1‑71 (MQ=255) cAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACg > 1:2032387/1‑71 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACa < 1:3699867/49‑1 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACgg > 1:1705896/1‑71 (MQ=255) aaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGt > 1:617399/1‑41 (MQ=255) aaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACgg > 1:3178845/1‑70 (MQ=255) aaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACgg > 1:837261/1‑70 (MQ=255) | CCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGG > minE/836414‑836550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |