| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,612 | T→C | 22.2% | Y444H (TAC→CAC) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,612 | 0 | T | C | 22.2% | 151.0 / 42.0 | 81 | Y444H (TAC→CAC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (35/28); new base C (11/7); total (46/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
TGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGAACTGGCA > minE/836545‑836680 | tGACGGTAAAGTTGATACCCGTGCTCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTAcc > 1:2997884/1‑71 (MQ=255) aCGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGCACCGt < 1:863890/71‑1 (MQ=255) cGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGt > 1:1878755/1‑70 (MQ=255) aaaGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCt > 1:1780408/1‑71 (MQ=255) aaGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCt < 1:3376845/70‑1 (MQ=255) aaGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTg < 1:1157408/71‑1 (MQ=255) gATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGa < 1:961623/70‑1 (MQ=255) aTACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGAtt < 1:1500196/71‑1 (MQ=255) tACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTg > 1:3216574/1‑71 (MQ=255) aCCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGc > 1:1367429/1‑71 (MQ=255) gTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAAc > 1:2233303/1‑71 (MQ=255) gcTGGAAGAGGTCGGTCTGACCGAAGCCCAGGGACAGGAGATGTACCGTTATCt < 1:1776727/54‑1 (MQ=255) gcTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCt < 1:418949/54‑1 (MQ=255) gcTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGc < 1:2145397/57‑1 (MQ=255) cTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACg < 1:1261099/70‑1 (MQ=255) cTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACg < 1:334060/70‑1 (MQ=255) tGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGtt < 1:930902/48‑1 (MQ=255) ggAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACg > 1:1206168/1‑68 (MQ=255) gAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACg > 1:162032/1‑67 (MQ=255) gAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACGAAGa < 1:3374650/71‑1 (MQ=255) aaGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACg > 1:2574324/1‑66 (MQ=255) agagGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACg > 1:497729/1‑65 (MQ=255) gagGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGt > 1:3497204/1‑37 (MQ=255) agGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGCACCGTTATCTg > 1:1629306/1‑47 (MQ=255) agGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGCACCGTTATCTg > 1:3188181/1‑47 (MQ=255) agGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGCACCGTTATCTg > 1:2300576/1‑47 (MQ=255) tcggtcTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCttt < 1:1235938/71‑1 (MQ=255) cggtcTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGa > 1:1645453/1‑47 (MQ=255) cggtcTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGa > 1:2199123/1‑47 (MQ=255) cggtcTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCtt > 1:47269/1‑69 (MQ=255) cggtcTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTtg > 1:3094574/1‑71 (MQ=255) gtcTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTtgtg < 1:824376/71‑1 (MQ=255) gtcTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTtgtg < 1:1467970/71‑1 (MQ=255) gtcTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTtgtg < 1:449511/71‑1 (MQ=255) gtcTGACCGAAGCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACGaa < 1:3922392/59‑1 (MQ=255) tcTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTgtgg > 1:3314841/1‑71 (MQ=255) tcTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTgtgg > 1:2564295/1‑71 (MQ=255) cTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTgtggt > 1:152027/1‑71 (MQ=255) tGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTg > 1:2235632/1‑45 (MQ=255) tGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGaa < 1:3489769/56‑1 (MQ=255) tGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTgtggtg > 1:558584/1‑71 (MQ=255) aCCGAAGCCCAGGCACAGGAGATGTACCGTTATCTg > 1:2699011/1‑36 (MQ=255) aCCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGAt < 1:1538584/41‑1 (MQ=255) aCCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGAt < 1:3538876/41‑1 (MQ=255) aCCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTaa > 1:584162/1‑47 (MQ=255) aCCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGAt > 1:1432204/1‑57 (MQ=255) ccGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGcc > 1:958585/1‑70 (MQ=255) ccGAAGCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGcc > 1:1199309/1‑70 (MQ=255) cGAAGCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTtg < 1:2520652/62‑1 (MQ=255) cGAAGCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTtg < 1:2380132/62‑1 (MQ=255) gAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTaa > 1:2136182/1‑44 (MQ=255) gAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGag < 1:3142984/71‑1 (MQ=255) aaGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGag > 1:1893541/1‑70 (MQ=255) aaGCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTg > 1:98782/1‑39 (MQ=255) aaGCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACGaa < 1:515421/50‑1 (MQ=255) aaGCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGag > 1:1270479/1‑70 (MQ=255) aGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGag > 1:265921/1‑69 (MQ=255) gCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATc > 1:1218274/1‑52 (MQ=255) gCCCAGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGagtag > 1:174001/1‑71 (MQ=255) cAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTgtggtg < 1:2709056/60‑1 (MQ=255) cAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGagt > 1:1714251/1‑66 (MQ=255) aGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCAt > 1:1651741/1‑71 (MQ=255) aGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCAt > 1:638637/1‑71 (MQ=255) aGGCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCAt > 1:182283/1‑71 (MQ=255) gCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTgtgg > 1:505876/1‑55 (MQ=255) gCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATc < 1:588006/70‑1 (MQ=255) gCACAGGAGATGCACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCg < 1:156424/71‑1 (MQ=255) aGGAGATTTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCttt > 1:902816/1‑47 (MQ=255) aGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGaa > 1:214990/1‑71 (MQ=255) aGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGa < 1:568056/70‑1 (MQ=255) gagaTGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGAAc > 1:1729829/1‑70 (MQ=255) gagaTGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGAACt < 1:677847/71‑1 (MQ=255) gagaTGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGAACt > 1:1279638/1‑71 (MQ=255) gagaTGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGAACt > 1:1160337/1‑71 (MQ=255) gagaTGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCGTTGTGGTGCCGAGTAGTCATCGTGAACt < 1:568678/71‑1 (MQ=255) gagaTGTACAGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGAAc > 1:2268586/1‑70 (MQ=255) agaTGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGAACTg < 1:803686/71‑1 (MQ=255) agaTGCACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGAACTg > 1:2790088/1‑71 (MQ=255) gaTGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGcc < 1:270943/51‑1 (MQ=255) tGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCa < 1:57929/58‑1 (MQ=255) tGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGACCTGGCa < 1:511603/71‑1 (MQ=255) tGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGAACTGGCa < 1:3826428/71‑1 (MQ=255) tACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGa < 1:1774241/62‑1 (MQ=255) | TGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGTAGTCATCGTGAACTGGCA > minE/836545‑836680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |