| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 204,556 | A→G | 100% | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 204,556 | 0 | A | G | 100.0% | 185.9 / NA | 62 | intergenic (+156/‑28) | alaV/rrlH | tRNA‑Ala/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (35/27); total (35/27) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT > minE/204491‑204623 | aaaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc < 1:208490/71‑1 (MQ=9) aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa < 1:2568704/71‑1 (MQ=255) ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa > 1:3688905/1‑69 (MQ=255) tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:1392035/70‑1 (MQ=255) gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:2323940/70‑1 (MQ=255) aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa > 1:2536449/1‑70 (MQ=255) acTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCt < 1:195645/71‑1 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:3817665/1‑71 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg > 1:332373/1‑71 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg > 1:2611497/1‑71 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg < 1:2138633/71‑1 (MQ=255) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:3130730/1‑70 (MQ=25) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:1858693/1‑70 (MQ=25) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa > 1:993210/1‑51 (MQ=255) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg < 1:2466782/63‑1 (MQ=25) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:1132867/1‑69 (MQ=25) aGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg > 1:1861921/1‑71 (MQ=21) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:2434665/1‑69 (MQ=25) tCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:327633/1‑60 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc > 1:2512618/1‑47 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc > 1:3097743/1‑47 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc > 1:2614614/1‑47 (MQ=255) gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg < 1:3572672/70‑1 (MQ=25) gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg < 1:3474191/70‑1 (MQ=25) gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg < 1:689009/70‑1 (MQ=25) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt < 1:2188858/50‑1 (MQ=255) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt < 1:1649193/50‑1 (MQ=255) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc < 1:3175320/71‑1 (MQ=21) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCGAGAAACATCTTc > 1:1143203/1‑49 (MQ=11) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg > 1:2578747/1‑50 (MQ=255) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTa < 1:2148629/71‑1 (MQ=21) gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt > 1:1534779/1‑69 (MQ=25) gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt > 1:1249111/1‑69 (MQ=25) ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:1117239/1‑59 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:2434833/35‑1 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt < 1:2364204/64‑1 (MQ=25) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:3919776/69‑1 (MQ=25) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTac < 1:2901577/70‑1 (MQ=25) aaTTTTCGCAACACGATGATGGATCGCAAGAAACATc < 1:1749396/37‑1 (MQ=255) aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa > 1:3369659/1‑49 (MQ=255) aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:3566370/66‑1 (MQ=25) ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg < 1:1375511/71‑1 (MQ=21) ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg > 1:3575004/1‑71 (MQ=21) ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg > 1:2266522/1‑71 (MQ=21) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt < 1:3155149/69‑1 (MQ=25) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc < 1:482091/71‑1 (MQ=21) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc < 1:1601206/71‑1 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGc > 1:1558225/1‑54 (MQ=255) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc > 1:1091849/1‑71 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc > 1:1672472/1‑71 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc > 1:1177620/1‑71 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc > 1:3219746/1‑71 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc > 1:230552/1‑71 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc > 1:610220/1‑71 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc > 1:614141/1‑71 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc > 1:2772308/1‑71 (MQ=21) acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg < 1:1645201/38‑1 (MQ=255) acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt > 1:278047/1‑71 (MQ=21) cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg > 1:2845123/1‑71 (MQ=21) gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt > 1:107968/1‑47 (MQ=255) gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg < 1:3925653/71‑1 (MQ=21) atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGc < 1:1564299/71‑1 (MQ=21) tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt < 1:3535631/58‑1 (MQ=255) tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg > 1:330614/1‑69 (MQ=25) gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:3151695/1‑53 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAg < 1:2941969/70‑1 (MQ=25) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGt < 1:2783215/71‑1 (MQ=21) | AAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT > minE/204491‑204623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |