| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 871,512 | T→C | 10.4% | I504T (ATT→ACT) | topA → | DNA topoisomerase I, omega subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 871,512 | 0 | T | C | 10.4% | 175.8 / 10.6 | 67 | I504T (ATT→ACT) | topA | DNA topoisomerase I, omega subunit |
| Reads supporting (aligned to +/- strand): ref base T (20/40); new base C (2/5); total (22/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
GCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTC > minE/871444‑871579 | gCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCAtt > 1:705937/1‑70 (MQ=255) tAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATc > 1:292143/1‑71 (MQ=255) aaaGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCACTCAGGATc < 1:240537/70‑1 (MQ=255) aaaGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCAACCTATGCGTCGATCATTTCGACCATTCAGGATc > 1:2239606/1‑70 (MQ=255) aaGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGt < 1:3539840/71‑1 (MQ=255) aaGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGt < 1:3165828/71‑1 (MQ=255) gCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGc > 1:577191/1‑70 (MQ=255) gCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCt < 1:1287335/71‑1 (MQ=255) aaaaaCGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTg > 1:778427/1‑71 (MQ=255) aaaaCGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGc < 1:3086369/71‑1 (MQ=255) aaaaCGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCACTCAGGATCGTGGCTACGTGc < 1:196884/71‑1 (MQ=255) aaaCGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCg < 1:3395628/71‑1 (MQ=255) aaCGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGa < 1:2458617/71‑1 (MQ=255) tcgcgGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCg < 1:2512756/68‑1 (MQ=255) aCGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAg > 1:3395682/1‑71 (MQ=255) aCGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAg > 1:2937217/1‑71 (MQ=255) cgcgGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAg > 1:3659015/1‑70 (MQ=255) gTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCACTCAGGATCg < 1:3749629/52‑1 (MQ=255) aTCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCACTCAGGATCGTg > 1:1325819/1‑52 (MQ=255) aTCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCACTCAGGATCGTg > 1:1321122/1‑52 (MQ=255) tCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGaaaa < 1:1881674/70‑1 (MQ=255) tCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAt > 1:2555946/1‑71 (MQ=255) ggTCGTCCGTTTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAtcg > 1:1526481/1‑71 (MQ=255) ggTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAtcg > 1:347542/1‑71 (MQ=255) ggTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAtcg < 1:3635669/71‑1 (MQ=255) tcgtcCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAtcgtc < 1:1918246/71‑1 (MQ=255) tcgtcCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAtcgtc < 1:1396576/71‑1 (MQ=255) gtccgtcTACCTATGCGTCGATCATTTCGACCACTCAGGATCGTGGCTACGTGCGAGTAGAAAATcgtcgt < 1:1622344/71‑1 (MQ=255) tccgtcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGt < 1:1359483/51‑1 (MQ=255) tccgtcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGa < 1:3654075/60‑1 (MQ=255) tccgtcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATcgtcgt < 1:2103126/70‑1 (MQ=255) cgtcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGTCTACGTGCGAGTAGAAAATCGTCGttt < 1:1409827/70‑1 (MQ=255) gtcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGc < 1:2835657/50‑1 (MQ=255) gtcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATcgtcgt > 1:1950373/1‑67 (MQ=255) gtcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCt < 1:658056/71‑1 (MQ=255) gtcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCt < 1:3626620/71‑1 (MQ=255) tcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGc < 1:791843/49‑1 (MQ=255) tcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTa > 1:662853/1‑71 (MQ=255) tcTACCTATGCGTCGATCATTTCGACCACTCAGGATCg < 1:1269626/38‑1 (MQ=255) tACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATg > 1:1376223/1‑71 (MQ=255) aCCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGc > 1:1848318/1‑71 (MQ=255) aCCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGc > 1:1850424/1‑71 (MQ=255) ccTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATcgtcgt < 1:2683224/62‑1 (MQ=255) ccTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTAt < 1:1526517/68‑1 (MQ=255) ccTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATg < 1:646660/69‑1 (MQ=255) ccTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATg < 1:2637198/69‑1 (MQ=255) gCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAAtcgtc > 1:409145/1‑55 (MQ=255) cGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGaaaa < 1:907079/70‑1 (MQ=255) gTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGa < 1:1923900/45‑1 (MQ=255) gTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGaaaaaa < 1:405341/71‑1 (MQ=255) tCGATCATTTCGACCATTCAGGATCGTGGCTACGt < 1:487850/35‑1 (MQ=255) tCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAAt < 1:2601900/71‑1 (MQ=255) tCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAAt < 1:3795223/71‑1 (MQ=255) tCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAAt < 1:106694/71‑1 (MQ=255) aTCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGaaaaa < 1:1123033/66‑1 (MQ=255) tCATTTCGACCATTCAGGATCGTGGCTACGTGCGAg < 1:3939040/36‑1 (MQ=255) cATTTCGACCATTCAGGATCGTGGCTACGTGCGAg > 1:2851118/1‑35 (MQ=255) aTTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATcgtcgt < 1:2708568/48‑1 (MQ=255) aTTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATcgtcgt < 1:1280791/48‑1 (MQ=255) aTTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCg < 1:3709316/70‑1 (MQ=255) tttCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGa > 1:2594939/1‑70 (MQ=255) tttCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGa > 1:2453245/1‑70 (MQ=255) tttCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGa > 1:92984/1‑70 (MQ=255) gACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCg < 1:3659459/71‑1 (MQ=255) gACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCCGAAAAAATGGGCGAAATCg < 1:1199626/71‑1 (MQ=255) gACCATTCAGGATCGTGGCTACGTACGTGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCg < 1:2498344/71‑1 (MQ=255) ccATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTc < 1:3159026/71‑1 (MQ=255) ccATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTc < 1:786180/71‑1 (MQ=255) ttCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGaaaa < 1:1548237/52‑1 (MQ=255) ttCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGaaaa < 1:1348925/52‑1 (MQ=255) | GCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTC > minE/871444‑871579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |