| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 890,121 | T→C | 28.6% | intergenic (‑128/+240) | fabI ← / ← ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 890,121 | 0 | T | C | 28.6% | 57.1 / 27.3 | 42 | intergenic (‑128/+240) | fabI/ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/23); new base C (4/8); total (11/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
TAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGG > minE/890052‑890185 | tAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGt < 1:238150/70‑1 (MQ=255) aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTcc > 1:3929926/1‑71 (MQ=255) tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGt < 1:1486473/67‑1 (MQ=255) aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGAc > 1:2292756/1‑71 (MQ=255) aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCg < 1:3384936/71‑1 (MQ=255) aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCg < 1:715483/71‑1 (MQ=255) cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGAcctg < 1:3761446/71‑3 (MQ=255) cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCg > 1:560078/1‑70 (MQ=255) cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCgg < 1:2731966/71‑1 (MQ=255) gTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAg < 1:1424921/71‑1 (MQ=255) cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAg > 1:1531677/1‑71 (MQ=255) cAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTg < 1:2065692/71‑1 (MQ=255) tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGc < 1:1414533/71‑1 (MQ=255) aaaCGGTTATTATAATCTACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGa < 1:379112/53‑1 (MQ=255) aaCGGTTATTATAATCACCCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGAcc > 1:3425928/1‑54 (MQ=255) aaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCGGAGCAGGCTGCGGCAg > 1:2716026/1‑71 (MQ=255) aCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgg < 1:2411881/71‑1 (MQ=255) cGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAggg < 1:2514551/71‑1 (MQ=255) ggTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCGGAGCAGGCTGCGGCAgggg < 1:3933622/71‑1 (MQ=255) gTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCGGAGCAGGCTGCGGCAggggg < 1:290015/71‑1 (MQ=255) ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgggggg < 1:2677782/71‑1 (MQ=255) ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgggggg < 1:507280/71‑1 (MQ=255) ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCGGAGCAGGCTGCGGCAgggggg < 1:2974543/71‑1 (MQ=255) ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCa > 1:2494361/1‑62 (MQ=255) taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCtt > 1:2384180/1‑71 (MQ=255) aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCtttt < 1:2031373/71‑1 (MQ=255) aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCtttt < 1:2387766/71‑1 (MQ=255) aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCttt < 1:278093/70‑1 (MQ=255) aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCttt < 1:3736833/70‑1 (MQ=255) tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgggg > 1:3471399/1‑60 (MQ=255) tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgggg > 1:1880953/1‑60 (MQ=255) cTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTc < 1:3131148/71‑1 (MQ=255) cTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCgg < 1:494759/45‑1 (MQ=255) tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCtt < 1:3138295/58‑1 (MQ=255) tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCt < 1:2850738/69‑1 (MQ=255) gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAccc < 1:2096936/71‑1 (MQ=255) tAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAggg < 1:3166798/43‑1 (MQ=255) gCTATAGTTGCCAGGCCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCctc < 1:3553722/70‑1 (MQ=255) cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgggg < 1:1583862/41‑1 (MQ=255) cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgggg < 1:3863555/41‑1 (MQ=255) tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAggggggg < 1:2637437/43‑1 (MQ=255) ttGCCAGGCCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAc > 1:1449955/1‑59 (MQ=255) ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg < 1:3093127/70‑1 (MQ=255) ccAGGCCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg < 1:3353720/70‑1 (MQ=255) | TAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGG > minE/890052‑890185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |