Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 63,049 T→C 7.8% N137S (AAT→AGT)  polB ← DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE63,0490TC 7.8% 142.2 / 3.5 51N137S (AAT→AGT) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (30/17);  new base C (2/2);  total (32/19)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

TCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGA  >  minE/62986‑63117
                                                               |                                                                    
tCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                >  1:926905/1‑70 (MQ=255)
tCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                >  1:520038/1‑70 (MQ=255)
tCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                >  1:3108879/1‑70 (MQ=255)
 cAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                >  1:101153/1‑69 (MQ=255)
 cAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                >  1:1605203/1‑69 (MQ=255)
 cAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                >  1:3644107/1‑69 (MQ=255)
          taGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCa                                                        <  1:640950/68‑1 (MQ=255)
          taGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATtg                                                     <  1:1257820/71‑1 (MQ=255)
          taGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATtg                                                     <  1:3915707/71‑1 (MQ=255)
            gAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATtgt                                                    <  1:34236/70‑1 (MQ=255)
              aaCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCa                                                 <  1:1748270/71‑1 (MQ=255)
               aCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCat                                                <  1:3799938/71‑1 (MQ=255)
               aCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCa                                                 >  1:1490486/1‑70 (MQ=255)
               aCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCa                                                 >  1:2907924/1‑70 (MQ=255)
                 ccACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCatat                                              <  1:1234864/71‑1 (MQ=255)
                   aCTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCa                                            >  1:2055238/1‑71 (MQ=255)
                      tGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCAcc                                          >  1:2833113/1‑70 (MQ=255)
                        aGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                <  1:2809462/46‑1 (MQ=255)
                        aGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                <  1:3897010/46‑1 (MQ=255)
                        aGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGa                                                                <  1:2882542/46‑1 (MQ=255)
                            gcggACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGAcc                                   >  1:3209140/1‑71 (MQ=255)
                                  gATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACAcc                             <  1:384981/71‑1 (MQ=255)
                                     aGTCGGGATGCGGTTTCAGACGGGCATTAACGATAg                                                             <  1:3559714/36‑1 (MQ=255)
                                     aGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCAcc                                          >  1:2832398/1‑55 (MQ=255)
                                     aGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggt                          >  1:2264294/1‑71 (MQ=255)
                                      gTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggt                          >  1:3730974/1‑70 (MQ=255)
                                       tCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtga                        <  1:945813/71‑1 (MQ=255)
                                        cGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgag                       >  1:3543699/1‑71 (MQ=255)
                                         gggATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgagg                      >  1:2835111/1‑71 (MQ=255)
                                         gggATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgagg                      >  1:1573966/1‑71 (MQ=255)
                                               cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCata                                               >  1:1244860/1‑40 (MQ=255)
                                               cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCata                                               >  1:892390/1‑40 (MQ=255)
                                               cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCata                                               >  1:3665811/1‑40 (MQ=255)
                                               cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATa                 >  1:3241377/1‑70 (MQ=255)
                                                ggTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCca                                 >  1:386437/1‑53 (MQ=255)
                                                ggTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATaaa               >  1:2351909/1‑71 (MQ=255)
                                                    tCAGACGGGGATTAACGATAGTGCCATTGTGCATATCACCCTc                                       >  1:2619606/1‑43 (MQ=255)
                                                    tCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGc           >  1:2082603/1‑71 (MQ=255)
                                                     cAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCt          >  1:328870/1‑71 (MQ=255)
                                                      aGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTc         >  1:2606051/1‑71 (MQ=255)
                                                        aCGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCa       <  1:215644/71‑1 (MQ=255)
                                                         cGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt      <  1:729795/71‑1 (MQ=255)
                                                         atggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt      <  1:3883913/69‑1 (MQ=38)
                                                         atggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt      <  1:697070/69‑1 (MQ=38)
                                                          tggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt      >  1:1882755/2‑70 (MQ=255)
                                                          tggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt      >  1:1814764/2‑70 (MQ=255)
                                                             cATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt      >  1:708358/1‑67 (MQ=255)
                                                             cATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa  <  1:2852539/71‑1 (MQ=255)
                                                             cATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa  <  1:1354054/71‑1 (MQ=255)
                                                              aTTAACGATAGTGCCATTGTGCATATCACCCTCGCCCCACACCGGTGAGGTGATAAACCGCTCCATCAGa  >  1:1959647/1‑70 (MQ=255)
                                                              aTTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt      >  1:2826842/1‑66 (MQ=255)
                                                               ttAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa  >  1:685845/1‑69 (MQ=255)
                                                               ttAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa  >  1:1030964/1‑69 (MQ=255)
                                                               |                                                                    
TCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGA  >  minE/62986‑63117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑