| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 910,897 | C→T | 22.8% | T46I (ACC→ATC) ‡ | ycjV → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 910,897 | 0 | C | T | 22.8% | 150.9 / 35.9 | 70 | T46I (ACC→ATC) ‡ | ycjV | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (35/19); new base T (7/9); total (42/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01 | |||||||||||
TCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAAT > minE/910828‑910967 | tCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGaccacc > 1:3612166/1‑71 (MQ=255) cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCAccc > 1:749968/1‑71 (MQ=255) cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCAccc > 1:1536046/1‑71 (MQ=255) aCCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTg < 1:833088/71‑1 (MQ=255) ccTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTg > 1:2592759/1‑70 (MQ=255) ggAAATTGCCGATAAAGAGTTCATTGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCa > 1:3473833/1‑71 (MQ=255) gAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTgcgc < 1:3613000/69‑1 (MQ=255) aaaTTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCAt > 1:136590/1‑70 (MQ=255) ccGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGcc < 1:1439924/71‑1 (MQ=255) tAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCggg > 1:1472186/1‑70 (MQ=255) aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCt > 1:546779/1‑71 (MQ=255) aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt < 1:1901000/71‑1 (MQ=255) aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt < 1:1452888/71‑1 (MQ=255) agagTTCATCGTGTTTGTCGGCCCGTCGTGCTGCGGTAAGTCGACCACCCTGCGCATGAt < 1:141095/60‑1 (MQ=255) agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTg < 1:1790141/71‑1 (MQ=255) gagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTg < 1:3865765/70‑1 (MQ=255) agTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTg > 1:2135672/1‑60 (MQ=255) agTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgag < 1:3901651/71‑1 (MQ=255) agTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCCCATGATTGCCGGGCTTgag < 1:3612556/71‑1 (MQ=255) gTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgag > 1:1360738/1‑70 (MQ=255) ttCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCAt < 1:3304866/53‑1 (MQ=255) ttCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCAt < 1:90573/53‑1 (MQ=255) ttCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCAt < 1:3753757/53‑1 (MQ=255) tCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGcc > 1:2015063/1‑59 (MQ=255) tCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGcc > 1:1330064/1‑59 (MQ=255) aTCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGAt < 1:2932579/71‑1 (MQ=255) gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg > 1:1005817/1‑71 (MQ=255) gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac > 1:1415278/1‑70 (MQ=9) gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCa < 1:631263/70‑1 (MQ=9) tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTgcgc < 1:810492/42‑1 (MQ=255) tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcgg > 1:2389796/1‑71 (MQ=255) ttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGa > 1:2596682/1‑45 (MQ=255) ttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggc > 1:734960/1‑71 (MQ=255) ttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggc > 1:3799680/1‑71 (MQ=255) tGTCGGCCCGTCGGGCTGCGGTAAGTCGCCCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcg > 1:3880915/1‑71 (MQ=255) tGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGa > 1:3012446/1‑44 (MQ=255) tGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgaggag < 1:3271633/61‑1 (MQ=255) tGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcgg > 1:2161284/1‑69 (MQ=255) gTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcgg > 1:277638/1‑71 (MQ=255) cGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcggc > 1:3742553/1‑70 (MQ=255) cGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcggc > 1:2329500/1‑70 (MQ=255) ggaccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCAccagcggcg < 1:1592846/67‑1 (MQ=2) ggaccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCAccagcggcg < 1:1619946/67‑1 (MQ=2) gCCCGTCGGGCTGCGGTAAGTCGACCCCCCTGCGCATGATAGc > 1:2104121/1‑43 (MQ=37) ccGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGAt > 1:1711046/1‑69 (MQ=255) ccGTCGGGCTGAGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGAt > 1:2792596/1‑69 (MQ=255) cGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTg < 1:402850/71‑1 (MQ=255) tctggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGtt > 1:2772037/4‑71 (MQ=9) ctggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGtt > 1:3500159/3‑70 (MQ=16) cGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt > 1:3393274/1‑70 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt < 1:2482425/69‑1 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa > 1:3199998/1‑71 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGAATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa > 1:405442/1‑71 (MQ=255) ggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCAt > 1:884731/1‑71 (MQ=16) gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca < 1:3264086/69‑3 (MQ=16) gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCAt < 1:2602541/70‑1 (MQ=255) cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttc < 1:1165107/67‑2 (MQ=255) cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttc < 1:3803538/67‑2 (MQ=255) tGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa > 1:757461/1‑71 (MQ=255) tGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa > 1:3366709/1‑71 (MQ=255) tGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTga < 1:3523680/41‑1 (MQ=16) tGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCg > 1:1239188/1‑70 (MQ=255) gCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGAc > 1:3785816/1‑71 (MQ=255) cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggtg < 1:1457940/71‑4 (MQ=255) cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt > 1:1997516/1‑68 (MQ=255) cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt > 1:3516723/1‑68 (MQ=255) gTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGAGGCGATCTGTTGATCGACGGc < 1:1072535/71‑1 (MQ=255) gTAAGTCGACAACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcggcg > 1:178822/1‑54 (MQ=255) aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa > 1:547885/1‑70 (MQ=255) aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa > 1:237389/1‑70 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTgag > 1:1078128/1‑35 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCaaa > 1:3891566/1‑71 (MQ=255) cGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg > 1:122709/1‑70 (MQ=255) ccaccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg < 1:2317138/71‑1 (MQ=255) cccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:182999/50‑1 (MQ=255) cccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat < 1:1210238/71‑1 (MQ=255) | TCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAAT > minE/910828‑910967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |