| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 910,923 | G→A | 31.3% | E55K (GAG→AAG) ‡ | ycjV → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 910,923 | 0 | G | A | 31.3% | 102.8 / 52.0 | 67 | E55K (GAG→AAG) ‡ | ycjV | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (31/15); new base A (9/12); total (40/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
ATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATAT > minE/910857‑910993 | aTCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGAt < 1:2932579/71‑1 (MQ=255) gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg > 1:1005817/1‑71 (MQ=255) gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac > 1:1415278/1‑70 (MQ=9) gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCa < 1:631263/70‑1 (MQ=9) tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcgg > 1:2389796/1‑71 (MQ=255) ttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggc > 1:3799680/1‑71 (MQ=255) ttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggc > 1:734960/1‑71 (MQ=255) tGTCGGCCCGTCGGGCTGCGGTAAGTCGCCCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcg > 1:3880915/1‑71 (MQ=255) tGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgaggag < 1:3271633/61‑1 (MQ=255) tGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcgg > 1:2161284/1‑69 (MQ=255) gTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcgg > 1:277638/1‑71 (MQ=255) cGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcggc > 1:3742553/1‑70 (MQ=255) cGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcggc > 1:2329500/1‑70 (MQ=255) ggaccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCAccagcggcg < 1:1592846/67‑1 (MQ=2) ggaccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCAccagcggcg < 1:1619946/67‑1 (MQ=2) ccGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGAt > 1:1711046/1‑69 (MQ=255) ccGTCGGGCTGAGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGAt > 1:2792596/1‑69 (MQ=255) cGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTg < 1:402850/71‑1 (MQ=255) tctggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGtt > 1:2772037/4‑71 (MQ=9) ctggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGtt > 1:3500159/3‑70 (MQ=16) cGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt > 1:3393274/1‑70 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt < 1:2482425/69‑1 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa > 1:3199998/1‑71 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGAATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa > 1:405442/1‑71 (MQ=255) ggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCAt > 1:884731/1‑71 (MQ=16) gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca < 1:3264086/69‑3 (MQ=16) gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCAt < 1:2602541/70‑1 (MQ=255) cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttc < 1:1165107/67‑2 (MQ=255) cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttc < 1:3803538/67‑2 (MQ=255) tGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa > 1:757461/1‑71 (MQ=255) tGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa > 1:3366709/1‑71 (MQ=255) tGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCg > 1:1239188/1‑70 (MQ=255) gCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGAc > 1:3785816/1‑71 (MQ=255) cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggtg < 1:1457940/71‑4 (MQ=255) cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt > 1:3516723/1‑68 (MQ=255) cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt > 1:1997516/1‑68 (MQ=255) gTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGAGGCGATCTGTTGATCGACGGc < 1:1072535/71‑1 (MQ=255) gTAAGTCGACAACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcggcg > 1:178822/1‑54 (MQ=255) aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa > 1:237389/1‑70 (MQ=255) aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa > 1:547885/1‑70 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCaaa > 1:3891566/1‑71 (MQ=255) cGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg > 1:122709/1‑70 (MQ=255) ccaccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg < 1:2317138/71‑1 (MQ=255) tacTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat > 1:456523/3‑71 (MQ=255) tacTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat > 1:2320139/3‑71 (MQ=255) cccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:182999/50‑1 (MQ=255) cccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat < 1:1210238/71‑1 (MQ=255) ccTGCGCATGATTGCCGGGCTTGAGGAGATCAGAGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat > 1:2466108/1‑70 (MQ=255) acTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat < 1:265057/69‑1 (MQ=255) acTGCGCATGATTGCCGGGCTTGAGACGATCACCACCGGCGACCTGTTCATCGGTGAGAAACggatgaat < 1:258972/69‑1 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGAc > 1:2870064/1‑71 (MQ=255) tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGacc < 1:2039685/42‑3 (MQ=25) cgcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGt < 1:3079900/71‑1 (MQ=255) gcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGAcact < 1:3139622/71‑4 (MQ=255) cATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:3528822/71‑1 (MQ=255) aTGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc > 1:508475/1‑70 (MQ=255) ttGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAatgaatga > 1:3324965/1‑61 (MQ=255) ttGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc > 1:185290/1‑70 (MQ=255) gCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa < 1:411602/70‑1 (MQ=255) ccGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaaa < 1:3546863/71‑1 (MQ=255) ccGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaaa < 1:3094100/71‑1 (MQ=255) cGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAg > 1:787759/1‑71 (MQ=255) cGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGAAGGCAAACGAATGAATGACGTTCCAGCCa > 1:3686585/1‑68 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaa < 1:2016084/52‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:3383577/60‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCa < 1:2495902/71‑1 (MQ=255) cTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACg > 1:2175551/1‑71 (MQ=255) ttGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACg > 1:299627/1‑70 (MQ=255) gaggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCa < 1:2967968/70‑1 (MQ=255) gagaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAatat < 1:2144879/71‑1 (MQ=255) | ATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATAT > minE/910857‑910993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |