| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 910,954 | G→A | 6.7% | G65D (GGC→GAC) ‡ | ycjV → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 910,954 | 0 | G | A | 6.7% | 226.5 / 4.7 | 74 | G65D (GGC→GAC) ‡ | ycjV | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (32/37); new base A (2/3); total (34/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
GTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTACGCGTTGT > minE/910885‑911022 | gTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGAGGCGATCTGTTGATCGACGGc < 1:1072535/71‑1 (MQ=255) aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa > 1:547885/1‑70 (MQ=255) aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa > 1:237389/1‑70 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCaaa > 1:3891566/1‑71 (MQ=255) cGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg > 1:122709/1‑70 (MQ=255) ccaccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg < 1:2317138/71‑1 (MQ=255) tacTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat > 1:456523/3‑71 (MQ=255) tacTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat > 1:2320139/3‑71 (MQ=255) cccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat < 1:1210238/71‑1 (MQ=255) ccTGCGCATGATTGCCGGGCTTGAGGAGATCAGAGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat > 1:2466108/1‑70 (MQ=255) acTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat < 1:265057/69‑1 (MQ=255) acTGCGCATGATTGCCGGGCTTGAGACGATCACCACCGGCGACCTGTTCATCGGTGAGAAACggatgaat < 1:258972/69‑1 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGAc > 1:2870064/1‑71 (MQ=255) cgcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGt < 1:3079900/71‑1 (MQ=255) gcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGAcact < 1:3139622/71‑4 (MQ=255) cATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:3528822/71‑1 (MQ=255) aTGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc > 1:508475/1‑70 (MQ=255) ttGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAatgaatga > 1:3324965/1‑61 (MQ=255) ttGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc > 1:185290/1‑70 (MQ=255) gCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa < 1:411602/70‑1 (MQ=255) ccGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaaa < 1:3094100/71‑1 (MQ=255) ccGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaaa < 1:3546863/71‑1 (MQ=255) cGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAg > 1:787759/1‑71 (MQ=255) cGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGAAGGCAAACGAATGAATGACGTTCCAGCCa > 1:3686585/1‑68 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaa < 1:2016084/52‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:3383577/60‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCa < 1:2495902/71‑1 (MQ=255) cTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACg > 1:2175551/1‑71 (MQ=255) ttGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACg > 1:299627/1‑70 (MQ=255) gaggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCa < 1:2967968/70‑1 (MQ=255) gagaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAatat < 1:2144879/71‑1 (MQ=255) aTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCa < 1:529555/51‑1 (MQ=255) aTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAg > 1:1035646/1‑70 (MQ=255) aTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGc > 1:2779520/1‑71 (MQ=255) tCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCg < 1:2536765/71‑1 (MQ=255) tCAGCGGCGGCGATCTGTTGATCGACGGAAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCg < 1:736420/71‑1 (MQ=255) cAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCa > 1:1083363/1‑49 (MQ=255) cAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCa > 1:753462/1‑49 (MQ=255) cAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCa > 1:1745553/1‑49 (MQ=255) cAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGa > 1:231175/1‑71 (MQ=255) cggcggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACg > 1:2261490/1‑57 (MQ=255) cggcggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACg > 1:2544544/1‑57 (MQ=255) gcggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa < 1:2593526/48‑1 (MQ=255) cggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgtgt < 1:2962957/71‑1 (MQ=255) cggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgtgt < 1:79538/71‑1 (MQ=255) cgATCTGTTGATCGAAGGCAAACGAATGAATGACGTTc > 1:3699000/1‑38 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:555242/37‑1 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:962586/37‑1 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:2532786/37‑1 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTc < 1:229881/37‑1 (MQ=255) aTCTGTTGATAGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc > 1:3402184/1‑68 (MQ=255) cTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaaa > 1:3152292/1‑42 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAg > 1:820417/1‑42 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGc > 1:3271298/1‑43 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGc > 1:3808916/1‑43 (MQ=255) gTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAAc > 1:193248/1‑70 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGc < 1:1038810/41‑1 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGc < 1:2031648/41‑1 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAg < 1:2454579/66‑1 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACt < 1:1370355/70‑1 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACt < 1:3778973/70‑1 (MQ=255) tGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAc > 1:3875930/1‑71 (MQ=255) tGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAc > 1:36747/1‑71 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGAt < 1:3551494/54‑1 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGAt < 1:3630903/54‑1 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGAt < 1:2454733/54‑1 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAcg > 1:3837375/1‑71 (MQ=255) aTCGACGGCAAACGTATGTATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAcg < 1:3841818/70‑1 (MQ=255) aTCGACGGCAAACGAATGAATGACGTTCCATCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAcg < 1:2429763/70‑1 (MQ=255) aTCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAcg < 1:2520009/70‑1 (MQ=255) tCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAcgcg < 1:1898215/71‑1 (MQ=255) tCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAcgcg < 1:1135485/71‑1 (MQ=255) gACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAata > 1:3148655/1‑43 (MQ=255) cGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTACGCGTTGt < 1:3786313/71‑1 (MQ=255) ggCAAACGAATGAATGACGTTCCAGCCAAAGCACGc < 1:2038759/36‑1 (MQ=255) ggCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAg > 1:43542/1‑43 (MQ=255) | GTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTACGCGTTGT > minE/910885‑911022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |