| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 917,592 | A→G | 31.2% | L311L (TTA→TTG) | tyrR → | DNA‑binding transcriptional dual regulator, tyrosine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 917,592 | 0 | A | G | 31.2% | 118.9 / 78.7 | 96 | L311L (TTA→TTG) | tyrR | DNA‑binding transcriptional dual regulator, tyrosine‑binding |
| Reads supporting (aligned to +/- strand): ref base A (35/31); new base G (17/13); total (52/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
AGCAGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGG > minE/917522‑917662 | aGCAGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTa < 1:2542468/71‑1 (MQ=255)aGCAGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTa < 1:1599195/71‑1 (MQ=255) gCAGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTa < 1:2538038/70‑1 (MQ=255) aGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTg < 1:2286174/71‑1 (MQ=255) ggCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGc > 1:3298020/1‑71 (MQ=255) gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt > 1:248304/1‑71 (MQ=255) gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt > 1:2631603/1‑71 (MQ=255) gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt > 1:3527619/1‑71 (MQ=255) gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGtt > 1:1445880/1‑71 (MQ=255) gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGtt > 1:1854187/1‑71 (MQ=255) gAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGtt > 1:1433906/1‑71 (MQ=255) aaCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGt < 1:3823505/69‑1 (MQ=255) aaCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGt < 1:151303/69‑1 (MQ=255) aaCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCAAGGATGCAGGCGAAATTGCTGCGttt < 1:707026/71‑1 (MQ=255) aCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTc < 1:174056/71‑1 (MQ=255) aCGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTc > 1:193614/1‑71 (MQ=255) tggtTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACt > 1:2069729/1‑60 (MQ=255) tggtTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCtt > 1:2088330/1‑70 (MQ=255) gtTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAat > 1:549362/1‑71 (MQ=255) cGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAATTTGCTGCGTTTCCTTAatga < 1:3675231/70‑1 (MQ=255) cGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgat > 1:1811434/1‑71 (MQ=255) cGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgat > 1:2789217/1‑71 (MQ=255) cGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatga < 1:681513/70‑1 (MQ=255) ggTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgatg > 1:1805819/1‑71 (MQ=255) gTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatg < 1:1477941/67‑1 (MQ=255) gTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatg < 1:2695097/67‑1 (MQ=255) gCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTg > 1:1322089/1‑52 (MQ=255) ttGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCa < 1:1479268/67‑1 (MQ=255) ttGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACt < 1:1813710/69‑1 (MQ=255) tGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACtt > 1:3337659/1‑69 (MQ=255) tGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTc > 1:2365107/1‑71 (MQ=255) tGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTaa < 1:1716863/58‑1 (MQ=255) ggATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgat < 1:2151198/61‑1 (MQ=255) gATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTcc < 1:3943321/52‑1 (MQ=255) gATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTcc < 1:3708866/70‑1 (MQ=255) tGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTc > 1:2283878/1‑71 (MQ=255) gAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTa < 1:1911949/39‑1 (MQ=255) gAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTc < 1:3192619/70‑1 (MQ=255) aTAGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGt > 1:3688271/1‑71 (MQ=255) aTAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGtt < 1:3099269/43‑1 (MQ=255) tAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGtt < 1:2387309/71‑1 (MQ=255) tAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGtt > 1:3437998/1‑71 (MQ=255) aGGGGAAATGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTg > 1:2159475/1‑71 (MQ=255) aGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTg > 1:3526567/1‑71 (MQ=255) aGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTg > 1:3442437/1‑71 (MQ=255) ggggAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTgg < 1:2427562/71‑1 (MQ=255) ggAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg < 1:1155718/71‑1 (MQ=255) ggAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg < 1:3853865/71‑1 (MQ=255) gAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg > 1:144984/1‑70 (MQ=255) aaaTGTCACCACGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGaa < 1:2429075/71‑1 (MQ=255) aaaTGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg > 1:56664/1‑69 (MQ=255) gTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatgatg > 1:126089/1‑44 (MQ=255) gTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGAc > 1:2521764/1‑70 (MQ=255) tCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAatga < 1:1864749/41‑1 (MQ=255) tCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCa < 1:2042627/71‑1 (MQ=255) tCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCa > 1:3557216/1‑71 (MQ=255) caccacGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt > 1:128124/1‑71 (MQ=255) accacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt > 1:2795674/1‑70 (MQ=255) ccacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt > 1:572082/1‑69 (MQ=255) ccacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt > 1:3261174/1‑69 (MQ=255) cacGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCa < 1:485681/42‑1 (MQ=255) cacGGATGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCa < 1:3436997/42‑1 (MQ=255) cacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGaa > 1:3373629/1‑62 (MQ=255) cacGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt < 1:2830413/68‑1 (MQ=255) acGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACt > 1:430621/1‑43 (MQ=255) acGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACt > 1:2144166/1‑43 (MQ=255) cGGATGCAGGCGAAATTGCTGCGGTTCCTTAATGATGGCa < 1:820381/40‑1 (MQ=37) cGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGt < 1:2386133/71‑1 (MQ=255) cGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGt < 1:1826681/71‑1 (MQ=255) aTGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCa < 1:3441406/37‑1 (MQ=255) aTGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGAcc > 1:2357054/1‑61 (MQ=255) tGCAGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCAt < 1:2907132/71‑1 (MQ=255) aGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCAt > 1:648603/1‑68 (MQ=255) aGGCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCAt > 1:437773/1‑68 (MQ=255) aGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt < 1:3508739/59‑1 (MQ=255) aGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCAt < 1:3682791/59‑1 (MQ=255) ggCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTc > 1:2815529/1‑70 (MQ=255) ggCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCg < 1:559503/71‑1 (MQ=255) gCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGt < 1:2516872/39‑1 (MQ=255) gCGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGt < 1:151384/39‑1 (MQ=255) cGAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAt < 1:2211352/71‑1 (MQ=255) gAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCggg > 1:123837/1‑41 (MQ=255) gAAATTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtg > 1:1367852/1‑71 (MQ=255) gAAATTGCTGCGTTTCCTTAATGATGGCACTTTCAGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtg > 1:2381399/1‑71 (MQ=255) gAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtg < 1:3523451/71‑1 (MQ=255) aaTTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtgtg > 1:3886573/1‑71 (MQ=255) aaTTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtgtg > 1:1854686/1‑71 (MQ=255) aaTTGCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtg > 1:3552680/1‑69 (MQ=255) aaTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGAtgtg > 1:3526087/1‑71 (MQ=255) aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTc < 1:2801300/64‑1 (MQ=255) aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTc < 1:2374632/64‑1 (MQ=255) aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGc > 1:2964896/1‑71 (MQ=255) aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGc > 1:3852266/1‑71 (MQ=255) aTTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGc < 1:169504/71‑1 (MQ=255) ttACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCg < 1:2420187/43‑1 (MQ=255) tACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGa > 1:3196513/1‑52 (MQ=255) tACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCg > 1:3790808/1‑63 (MQ=255) tACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCgg > 1:1276041/1‑71 (MQ=255) tACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCgg > 1:1132932/1‑71 (MQ=255) aCTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCggg > 1:140104/1‑71 (MQ=255) | AGCAGGCGAACGGTGGTTCGGTGCTGTTGGATGAAATAGGGGAAATGTCACCACGGATGCAGGCGAAATTACTGCGTTTCCTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGG > minE/917522‑917662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |