| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 922,063 | C→T | 23.8% | R45* (CGA→TGA) | ycjZ → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 922,063 | 0 | C | T | 23.8% | 116.0 / 32.8 | 63 | R45* (CGA→TGA) | ycjZ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (16/32); new base T (7/8); total (23/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCTTTT > minE/921994‑922133 | aCTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAAc < 1:755643/70‑1 (MQ=255) gTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTg > 1:3505915/1‑71 (MQ=255) gTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGAtt < 1:830990/70‑1 (MQ=255) gTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGAt < 1:2413774/69‑1 (MQ=255) gcagcaGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGa < 1:3159423/66‑1 (MQ=255) agcagcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:2616261/68‑1 (MQ=255) agcagcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGAt > 1:3790493/1‑71 (MQ=255) gcagcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGAtt < 1:2851661/71‑1 (MQ=255) gcagcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGAtt < 1:2173338/71‑1 (MQ=255) cagcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTg < 1:1371325/71‑1 (MQ=255) cagcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTg > 1:1776522/1‑71 (MQ=255) gCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCtt > 1:3533507/1‑71 (MQ=255) ccTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGa < 1:1383423/61‑1 (MQ=255) ccTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGa < 1:2206413/61‑1 (MQ=255) ccTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTc < 1:3418618/71‑1 (MQ=255) ccTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTc < 1:392703/71‑1 (MQ=255) cTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCt < 1:2185447/71‑1 (MQ=255) cTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg > 1:413209/1‑58 (MQ=255) cTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCt < 1:2486172/71‑1 (MQ=255) aGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGAc > 1:3631828/1‑71 (MQ=255) aTGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgcg < 1:465677/71‑1 (MQ=255) aTGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgc > 1:3798343/1‑70 (MQ=255) aTGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgc > 1:391373/1‑70 (MQ=255) tGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgcg > 1:3019777/1‑70 (MQ=255) ggCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCt < 1:2624640/63‑1 (MQ=255) ggCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCt < 1:2727106/63‑1 (MQ=255) gcgcAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGaa < 1:1405595/70‑1 (MQ=255) gcAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCa < 1:3196656/71‑1 (MQ=255) gcAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCa < 1:608918/71‑1 (MQ=255) gcAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCa < 1:3710439/71‑1 (MQ=255) cAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTc < 1:287903/58‑1 (MQ=255) cAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTc < 1:1128979/58‑1 (MQ=255) cAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCa > 1:2026914/1‑70 (MQ=255) aaGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCAc > 1:3865094/2‑71 (MQ=255) aGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCAcg > 1:2397915/1‑71 (MQ=255) gTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTc > 1:2890750/1‑56 (MQ=255) gTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCAcgc < 1:1496967/71‑1 (MQ=255) cAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACg < 1:1345336/37‑1 (MQ=255) aGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCt > 1:1326561/1‑51 (MQ=255) aGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgcg < 1:3119374/60‑1 (MQ=255) aGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCa < 1:684867/71‑1 (MQ=255) cTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGc < 1:1474711/71‑1 (MQ=255) cTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAg < 1:2059933/70‑1 (MQ=255) tAAGCCAGATAGTGCGTCGTATACAAGAATGATTGGGAGTGCGGCTTCTGACGCGAACCACGCGCAGCgt < 1:781861/70‑1 (MQ=255) aGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgt > 1:449351/1‑71 (MQ=255) gCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGaa < 1:18025/54‑1 (MQ=255) ccAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTc < 1:1587941/71‑1 (MQ=255) aGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAAcc < 1:3332213/53‑1 (MQ=255) gATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCaa > 1:2927930/1‑71 (MQ=255) aTAGTGCTTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAAc > 1:3562004/1‑71 (MQ=255) aTAGTGCGTCGTATAGAAGAACGATTGGGATTGCg > 1:285409/1‑35 (MQ=255) gTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTcc < 1:2659712/65‑1 (MQ=255) gTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGa < 1:3898898/71‑1 (MQ=255) cgtcgtATAGAAGAATGATTGGGATTGCGGCTTCTGACGAGAACCACGCGCAGCGTTGTTCCAACTGAAGc > 1:1621949/1‑71 (MQ=255) tcgtATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgt < 1:52544/54‑1 (MQ=255) cgtATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAg < 1:2795165/50‑1 (MQ=255) taGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCa < 1:2770127/39‑1 (MQ=255) taGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAg > 1:3746899/1‑71 (MQ=255) taGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAg > 1:983499/1‑71 (MQ=255) taGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAg > 1:479245/1‑71 (MQ=255) agaagaACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGat > 1:2943199/1‑69 (MQ=255) aagaaCGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCggg < 1:2825429/64‑1 (MQ=255) aaCGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCtt < 1:3539737/71‑1 (MQ=255) aCGATTGGTATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCttt < 1:3230373/71‑1 (MQ=255) cGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCtttt < 1:145865/71‑1 (MQ=255) cGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCtttt < 1:37722/71‑1 (MQ=255) cGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCttt > 1:1016595/1‑70 (MQ=255) | ACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCTTTT > minE/921994‑922133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |