| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 943,008 | A→G | 8.4% | L45S (TTA→TCA) | ddpF ← | D‑Ala‑D‑Ala transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 943,008 | 0 | A | G | 8.4% | 194.8 / 7.8 | 71 | L45S (TTA→TCA) | ddpF | D‑Ala‑D‑Ala transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (31/34); new base G (4/2); total (35/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01 | |||||||||||
TTGCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAG > minE/942941‑943078 | ttGCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAgg > 1:636490/1‑71 (MQ=255) gcagcaTACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGtt > 1:2083656/1‑71 (MQ=255) agcaTACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTc < 1:379045/71‑1 (MQ=255) caTACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTcac > 1:770076/1‑71 (MQ=255) caTACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTcac < 1:1360101/71‑1 (MQ=255) caTACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTcac < 1:1309652/71‑1 (MQ=255) aTACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTcacc < 1:3694236/71‑1 (MQ=255) tACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTcacca > 1:1150504/1‑71 (MQ=255) cccATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCAcg > 1:2693791/1‑71 (MQ=255) cccATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCAcg > 1:3790407/1‑71 (MQ=255) ccATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGtt < 1:1060256/62‑1 (MQ=255) aTTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCAcggcg > 1:1251535/1‑71 (MQ=255) aTTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCATAAGGTTTCACCAcggcg < 1:3167158/71‑1 (MQ=255) ttAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGttt < 1:1574146/60‑1 (MQ=255) tAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTaa < 1:707004/54‑1 (MQ=255) aaaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAg > 1:2862578/1‑54 (MQ=255) aaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGAt < 1:2805051/70‑1 (MQ=255) aaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGAt < 1:3021506/70‑1 (MQ=255) aaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCt < 1:2332784/71‑1 (MQ=255) aGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTg > 1:1987387/1‑71 (MQ=255) tgtgCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTa > 1:702312/1‑70 (MQ=255) cGAGGGTGCTTTTGCCGCCGCCTGACTCGCCGACGATCCCTaa > 1:3438611/1‑43 (MQ=255) cGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATc > 1:2006115/1‑70 (MQ=255) cGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATc > 1:1129349/1‑70 (MQ=255) aGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAAt < 1:272011/71‑1 (MQ=255) gCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCAt > 1:556741/1‑71 (MQ=255) cTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCaa > 1:3765466/1‑64 (MQ=255) ttttGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCa > 1:2584739/1‑62 (MQ=255) ttGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCAt > 1:3093139/1‑67 (MQ=255) ttGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAAt > 1:3130415/1‑71 (MQ=255) ttGCCGCAGCCAGACTCGCCGACGATCCCTAAGGTTTcacc > 1:3800540/1‑41 (MQ=255) gCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCAt > 1:735470/1‑71 (MQ=255) cAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCAt > 1:1674032/1‑70 (MQ=255) cAGCCTGACT‑‑CCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGaa > 1:1598365/1‑71 (MQ=255) aGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATa > 1:2434836/1‑56 (MQ=255) ccTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGaa < 1:3904872/70‑1 (MQ=255) ccTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGt < 1:3319164/45‑1 (MQ=255) cTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGaa > 1:196275/1‑69 (MQ=255) tGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACa > 1:3142428/1‑70 (MQ=255) tGACTCGCCGACGATCCCTAAGGTTTCCCCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACAt > 1:3139400/1‑71 (MQ=255) aCTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATAc > 1:2090962/1‑51 (MQ=255) aCTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGt < 1:488863/71‑1 (MQ=255) ctctCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGtt < 1:2829197/71‑1 (MQ=255) cTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCa < 1:1880300/61‑1 (MQ=255) cTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGtt < 1:1999675/71‑1 (MQ=255) tCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGaa > 1:825452/1‑64 (MQ=255) tCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTc > 1:2356350/1‑71 (MQ=255) gCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGc > 1:1135963/1‑57 (MQ=255) gCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCg > 1:890548/1‑71 (MQ=255) gCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCg > 1:407784/1‑71 (MQ=255) ccGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTa < 1:3703795/51‑1 (MQ=255) ccGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTa < 1:197993/51‑1 (MQ=255) ccGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTa < 1:1699050/51‑1 (MQ=255) cgacgaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCAtt < 1:1040758/49‑1 (MQ=255) cgacgaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCAtt < 1:3035733/49‑1 (MQ=255) gacgaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAAc < 1:3825031/60‑1 (MQ=255) gacgaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAg < 1:2917897/71‑1 (MQ=255) acgaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTc < 1:2238170/65‑1 (MQ=255) acgaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGt < 1:3127794/71‑1 (MQ=255) acgaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGt < 1:1497391/71‑1 (MQ=255) gaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCa > 1:3862790/1‑43 (MQ=255) gaTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCg > 1:2792050/1‑65 (MQ=255) ttCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGtttt < 1:3654242/70‑1 (MQ=255) aTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGtttt < 1:1033380/71‑1 (MQ=255) aTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGtttt < 1:2064162/71‑1 (MQ=255) aTCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGtttt < 1:2795320/71‑1 (MQ=255) tCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGTCATGAACATGTTCCGTAGTTTTa < 1:1593707/71‑1 (MQ=255) ccTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTAcc < 1:652494/71‑1 (MQ=255) ccTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTAcc < 1:1935980/71‑1 (MQ=255) ccTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTAcc < 1:1328736/71‑1 (MQ=255) cTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAAc < 1:2309717/52‑1 (MQ=255) cTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTAcc > 1:2265317/1‑70 (MQ=255) cTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTAcc > 1:3777372/1‑70 (MQ=255) aaGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAAc > 1:1620456/1‑50 (MQ=255) aaGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAAc > 1:3673844/1‑50 (MQ=255) aaGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAg > 1:1973797/1‑71 (MQ=255) | TTGCAGCATACCCATTAAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTTCCGTAGTTTTACCAAG > minE/942941‑943078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |