| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 965,844 | A→T | 53.5% | V17D (GTT→GAT) | ydeM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 965,844 | 0 | A | T | 53.5% | 5.1 / 59.7 | 43 | V17D (GTT→GAT) | ydeM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (12/8); new base T (13/10); total (25/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
TTTCAGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTC > minE/965774‑965902 | tttCAGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTaaa > 1:1144079/1‑71 (MQ=255)tttCAGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTaaa > 1:429896/1‑71 (MQ=255) gTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGAt > 1:1923878/1‑71 (MQ=255) catcaatcCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTga > 1:1152027/1‑66 (MQ=255) atccatccATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGAtt > 1:1129341/1‑69 (MQ=255) tccatccaTTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACa < 1:3514408/71‑1 (MQ=255) tccatccaTTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACa < 1:1858740/71‑1 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaa < 1:37416/71‑1 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaa < 1:3935911/71‑1 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaa > 1:3104215/1‑70 (MQ=255) atTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGaaaa > 1:1519516/1‑71 (MQ=255) ttttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACt > 1:2559661/1‑71 (MQ=255) ttttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTAc > 1:729604/1‑61 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTg > 1:1099517/1‑71 (MQ=255) ttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTg > 1:546801/1‑70 (MQ=255) gAGTAAACTCCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTg < 1:1386468/65‑1 (MQ=255) gTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCtt < 1:732623/71‑1 (MQ=255) tAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAg < 1:2583518/65‑1 (MQ=255) aaaCTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTgg > 1:999083/1‑71 (MQ=255) aaCTGCGACTCTTTTTCAAGGTAAAAACAGTAAtcac > 1:1778015/1‑37 (MQ=255) aaCTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACAt > 1:2754660/1‑39 (MQ=255) aaCTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGc > 1:2584222/1‑71 (MQ=255) gCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGt > 1:2985114/1‑70 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGt < 1:3503556/69‑1 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGt < 1:106854/69‑1 (MQ=255) gACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAAc > 1:1026943/1‑71 (MQ=255) ctctTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACAt > 1:479193/1‑43 (MQ=255) ctctTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGt > 1:2603957/1‑71 (MQ=255) tctTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaa < 1:2803329/46‑1 (MQ=255) tctTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACg > 1:474004/1‑69 (MQ=255) tttttCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAAc < 1:3695520/47‑1 (MQ=255) tttttCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAAc > 1:2784247/1‑66 (MQ=255) tttttCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAAc > 1:2179576/1‑66 (MQ=255) ttCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCg > 1:1444346/1‑71 (MQ=255) cAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGc > 1:2017909/1‑70 (MQ=255) aaGTTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCt < 1:453463/70‑1 (MQ=255) aaGGTAAAAACAGTAATCACATTTGAGATTACATTGa < 1:3394254/37‑1 (MQ=255) aaGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCt < 1:648278/70‑1 (MQ=255) aaGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTa < 1:2706819/71‑1 (MQ=255) aGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAt < 1:293373/71‑1 (MQ=255) tAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtctc > 1:2406751/1‑71 (MQ=255) tAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGAATCGCTAtctc > 1:3282730/1‑71 (MQ=255) aaaaCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtct < 1:1545881/68‑1 (MQ=255) aaaaCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAt > 1:2285101/1‑66 (MQ=255) aaaCAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGt > 1:2211110/1‑55 (MQ=255) aGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTaa < 1:3119882/48‑1 (MQ=255) aaaCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCg < 1:126756/54‑1 (MQ=255) aaCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGt > 1:1970901/1‑47 (MQ=255) aaCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGt > 1:24235/1‑47 (MQ=255) | TTTCAGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTC > minE/965774‑965902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |