| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 974,990 | A→T | 54.6% | intergenic (+742/+250) | lsrB → / ← ydeH | AI2 transporter/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 974,990 | 0 | A | T | 54.6% | 24.8 / 103.5 | 77 | intergenic (+742/+250) | lsrB/ydeH | AI2 transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (23/12); new base T (17/25); total (40/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCAGGGTGCG > minE/974922‑975060 | aCTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACt > 1:3358226/1‑71 (MQ=255) cTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCtt > 1:3711763/1‑71 (MQ=255) cTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCtt > 1:324801/1‑71 (MQ=255) gCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCtt < 1:1190015/68‑1 (MQ=255) gCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCtt < 1:2817536/68‑1 (MQ=255) gCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGc > 1:1965568/1‑71 (MQ=255) aaaTTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGccc > 1:1077307/1‑71 (MQ=255) aaaTTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGccc > 1:3193967/1‑71 (MQ=255) ttCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCg < 1:1858115/71‑1 (MQ=255) cTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCg > 1:394305/1‑69 (MQ=255) gACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGc > 1:233961/1‑62 (MQ=255) gACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAAAAATGATAAATACTTCGc > 1:419501/1‑62 (MQ=255) acacCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGc < 1:2477036/70‑1 (MQ=255) acacCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGc < 1:1860882/70‑1 (MQ=255) cTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATaa < 1:1699199/71‑1 (MQ=255) gCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAg > 1:1820006/1‑63 (MQ=255) gCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCg < 1:3706898/65‑1 (MQ=255) gCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAAc > 1:3427146/1‑71 (MQ=255) cACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAg < 1:2172868/62‑1 (MQ=255) gACATGCTTCTTCATCATTAGCCGCTTTGACAATAATTATAAATTCTTCGCCCCCGTAGCGATAAACCGtt > 1:1182288/1‑71 (MQ=255) gACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGtt < 1:2253071/71‑1 (MQ=255) cATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTc < 1:3830649/71‑1 (MQ=255) aTGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGtt < 1:175568/68‑1 (MQ=255) gCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGt > 1:169892/1‑70 (MQ=255) cttcttCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGc > 1:3675220/1‑45 (MQ=255) ttcttcATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTaa > 1:2630398/1‑70 (MQ=255) cttcATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATa < 1:3923167/55‑1 (MQ=255) cttcATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAAc > 1:3165401/1‑58 (MQ=255) cttcATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATc < 1:503374/70‑1 (MQ=255) ttcATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAc > 1:2607807/1‑71 (MQ=255) ttcATCATTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAc > 1:2392169/1‑71 (MQ=255) catcatTAGCCGCTTTGACAATAATGATAACTTCTTCGccc < 1:1613492/41‑1 (MQ=38) atcatTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAcgc < 1:31204/70‑1 (MQ=255) atTAGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTc > 1:2270606/1‑70 (MQ=255) aGCCGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCAc > 1:1768521/1‑62 (MQ=255) aGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGtt > 1:84144/1‑52 (MQ=255) gCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACt < 1:3215864/71‑1 (MQ=255) cGCTTTGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTgg < 1:2119203/71‑1 (MQ=255) gCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGc > 1:2858394/1‑71 (MQ=255) cgatGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCt < 1:3045598/68‑1 (MQ=255) tttGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGt > 1:3944282/1‑60 (MQ=255) tttGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGt > 1:453000/1‑60 (MQ=255) ttGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGc > 1:1330057/1‑68 (MQ=255) ttGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCt > 1:1115448/1‑69 (MQ=255) ttGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCt > 1:565841/1‑69 (MQ=255) tGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaag > 1:1425173/1‑71 (MQ=255) tGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaag > 1:2420000/1‑71 (MQ=255) tGACAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaa < 1:1665392/70‑1 (MQ=255) tGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaa < 1:2164395/70‑1 (MQ=255) gACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaa > 1:3298989/1‑69 (MQ=255) aCAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagt < 1:459616/70‑1 (MQ=255) aCAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagt < 1:2245837/70‑1 (MQ=255) aCAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaa < 1:878866/68‑1 (MQ=255) aCAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagta < 1:3771611/71‑1 (MQ=255) cAATAATGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagtaa > 1:1665382/1‑71 (MQ=255) aataatGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagtaag < 1:334920/71‑1 (MQ=255) aataatGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagtaag < 1:3439713/71‑1 (MQ=255) ataatGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTaagtaagt < 1:3779200/71‑1 (MQ=255) taatGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTaagtaagt < 1:3377167/70‑1 (MQ=255) aatGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTg > 1:1443756/1‑71 (MQ=255) aatGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTg > 1:3909025/1‑71 (MQ=255) tGATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGc < 1:185613/70‑1 (MQ=255) gATAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCta > 1:3763870/1‑59 (MQ=255) gATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagtaa > 1:3557492/1‑64 (MQ=255) aTAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACt > 1:3546535/1‑53 (MQ=255) aTAAATTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCa < 1:1064024/70‑1 (MQ=255) aTAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCa < 1:2412748/70‑1 (MQ=255) aTAAATACTTCGCCACCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCa < 1:3907389/70‑1 (MQ=255) aaaTTCTTCGCCGCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCtaagt < 1:2241205/59‑1 (MQ=255) aaaTTCTTCGCCCCCGTAGCGATAAACCGTTTCGt < 1:727750/35‑1 (MQ=255) aaaTTCTTCGCCCCCGTAGCGATAAACCGTTTCGt < 1:1340235/35‑1 (MQ=255) aaaTTCTTCGCCCCCGTAGCGATAAACCGTTTCGt < 1:1344741/35‑1 (MQ=255) aaaTTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTaagtaagt > 1:3577168/1‑63 (MQ=255) aaTTCTTCGCCCCCGTAGCGATAAACCGTTTCGTAAt < 1:1813627/37‑1 (MQ=255) aTACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCAGGGTg > 1:1369654/1‑71 (MQ=255) aTACTTCGCCCCCGTAGCGATAAACCGTTTAGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCAGGGTg > 1:3050969/1‑71 (MQ=255) tACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCAGGGTGc > 1:2223315/1‑71 (MQ=255) aCTTCGCCCCCGTAGCGATAAACCGTTTCGTAATAACGCGTCCAACTGGCTAAGTAAGTTGCCAGGGTGCg > 1:914351/1‑71 (MQ=255) | ACTGGCAAATTCTGACACCTGCACGACATGCTTCTTCATCATTAGCCGCTTTGACAATAATGATAAATACTTCGCCCCCGTAGCGATAAACCGTTTCGTAATCACGCGTCCAACTGGCTAAGTAAGTTGCCAGGGTGCG > minE/974922‑975060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |