Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 980,233 T→A 7.7% K24N (AAA→AAT) ‡ cspI ← cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE980,2330TA 7.7% 181.7 / 6.6 73K24N (AAA→AAT) ‡cspIcold shock protein
Reads supporting (aligned to +/- strand):  ref base T (28/37);  new base A (2/3);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

AGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACA  >  minE/980167‑980303
                                                                  |                                                                      
aGTTAATGTTTTGAAATCCTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCtt                                                                       <  1:2524586/68‑1 (MQ=38)
  ttAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGc                                                                  >  1:2636296/1‑71 (MQ=255)
  ttAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGc                                                                  <  1:1732959/71‑1 (MQ=255)
  ttAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGc                                                                  <  1:3820552/71‑1 (MQ=255)
     aTGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtg                                                               <  1:1122383/71‑1 (MQ=255)
     aTGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtg                                                               <  1:3160479/71‑1 (MQ=255)
          ttGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtgatga                                                           <  1:2133592/70‑1 (MQ=255)
          ttGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGtgatga                                                           <  1:266283/70‑1 (MQ=255)
          ttGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGtgatga                                                           <  1:621809/70‑1 (MQ=255)
             aaaTCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAAcc                                                       <  1:3570037/71‑1 (MQ=255)
             aaaTCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAAcc                                                       <  1:3829845/71‑1 (MQ=255)
              aaTCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGc                                                                  >  1:3214165/1‑59 (MQ=255)
              aaTCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGc                                                                  >  1:107367/1‑59 (MQ=255)
              aaTCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAAcc                                                       <  1:79450/70‑1 (MQ=255)
               aTCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaa                                                     <  1:74153/71‑1 (MQ=255)
               aTCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaa                                                     <  1:1164842/71‑1 (MQ=255)
                tCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaa                                                     >  1:2807331/1‑70 (MQ=255)
                  gTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaaa                                                    <  1:1274742/69‑1 (MQ=255)
                     gCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCtt                                                                       >  1:317576/1‑47 (MQ=255)
                     gCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCtt                                               >  1:3253011/1‑71 (MQ=255)
                        ctGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGaa                                                          >  1:2839880/1‑57 (MQ=255)
                         tGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGtga                                                              >  1:2051136/1‑52 (MQ=255)
                         tGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAAcc                                                       >  1:1881350/1‑59 (MQ=255)
                           aaTTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                         >  1:2917685/1‑71 (MQ=255)
                           aaTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                         >  1:1779617/1‑71 (MQ=255)
                            aTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                        >  1:1634379/1‑71 (MQ=255)
                            aTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                        >  1:1237321/1‑71 (MQ=255)
                             ttGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGt                                                                <  1:1176248/46‑1 (MQ=255)
                                cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTa                                    <  1:2521743/71‑1 (MQ=255)
                                cTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTa                                    <  1:3571031/71‑1 (MQ=255)
                                  gagaAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaa                                                     <  1:2008069/52‑1 (MQ=255)
                                   agaAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGaaa                                                         >  1:1054258/1‑47 (MQ=255)
                                    gaAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtg                                                               >  1:3158922/1‑40 (MQ=255)
                                    gaAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaaaa                                                   >  1:2162911/1‑52 (MQ=255)
                                    gaAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc                                 >  1:3664975/1‑70 (MQ=255)
                                     aaaTGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCa                               >  1:1007396/1‑71 (MQ=255)
                                      aaTGGACAAACACATCTTTGCTGCCATCTTTCGGAGTGATGAAACCAAAACCTTTTTCAg                                         <  1:494509/60‑1 (MQ=25)
                                       atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt                             <  1:987032/68‑1 (MQ=255)
                                        tggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAggg                                       >  1:1273661/3‑60 (MQ=255)
                                        tggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAggg                                       >  1:232045/3‑60 (MQ=255)
                                        tAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                            <  1:2211814/71‑1 (MQ=255)
                                         gcaCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCAttt                            >  1:2548954/3‑70 (MQ=255)
                                          gACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaa                                                     >  1:1701962/1‑44 (MQ=255)
                                          gACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaa                                                     >  1:2141863/1‑44 (MQ=255)
                                          caCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCAtttta                          <  1:658298/70‑3 (MQ=255)
                                            cAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAAcc                                                       <  1:2518770/40‑1 (MQ=255)
                                            cAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCAc                         >  1:1534400/1‑70 (MQ=255)
                                            cAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACt                        <  1:1450160/71‑1 (MQ=255)
                                            cAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACt                        <  1:2728011/71‑1 (MQ=255)
                                             aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACTa                       <  1:3525091/71‑1 (MQ=255)
                                              aaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTaa                      <  1:1430417/71‑1 (MQ=255)
                                              aaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTaa                      <  1:3226106/71‑1 (MQ=255)
                                               acacATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAggg                                       >  1:2235155/1‑53 (MQ=255)
                                               acacATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTaaa                     >  1:3760251/1‑71 (MQ=255)
                                                 acgtCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAcc                   <  1:2092435/68‑1 (MQ=9)
                                                 acgtCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAc                    >  1:3505348/4‑70 (MQ=9)
                                                 acaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAcc                   <  1:3379269/71‑1 (MQ=255)
                                                         gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt           >  1:1066236/1‑71 (MQ=255)
                                                         gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt            <  1:2909648/70‑1 (MQ=255)
                                                          gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt          <  1:2536224/69‑1 (MQ=255)
                                                          gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt          <  1:3804793/69‑1 (MQ=255)
                                                           tgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                            <  1:2182225/52‑1 (MQ=255)
                                                           tgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAt                              <  1:531124/50‑1 (MQ=255)
                                                           agcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt          <  1:2864534/69‑1 (MQ=255)
                                                             ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCAc                         <  1:833754/53‑1 (MQ=255)
                                                             ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt        <  1:2761567/70‑1 (MQ=255)
                                                              cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                          <  1:3158695/35‑1 (MQ=255)
                                                              cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTaa                                   >  1:2866869/1‑42 (MQ=255)
                                                              cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCa                          >  1:400185/1‑51 (MQ=255)
                                                              cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa      <  1:1984849/71‑1 (MQ=255)
                                                               aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                         <  1:2663073/35‑1 (MQ=255)
                                                               aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc                    <  1:2536476/56‑1 (MQ=255)
                                                                tCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                        <  1:1487346/35‑1 (MQ=255)
                                                                tCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                        <  1:1740353/35‑1 (MQ=255)
                                                                 cTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg         >  1:1881533/1‑65 (MQ=255)
                                                                  tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt            >  1:3310296/1‑61 (MQ=255)
                                                                  tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa  >  1:1521667/1‑71 (MQ=255)
                                                                  |                                                                      
AGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACA  >  minE/980167‑980303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: