| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,944 | G→A | 14.3% | intergenic (‑36/‑70) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,944 | 0 | G | A | 14.3% | 175.8 / 18.4 | 70 | intergenic (‑36/‑70) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (22/38); new base A (4/6); total (26/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
AAGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGG > minE/217876‑218011 | aaGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCagag > 1:3516243/1‑69 (MQ=255) aGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAg > 1:2097651/1‑71 (MQ=255) gAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGt < 1:3020782/71‑1 (MQ=255) aaaaTTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGtt > 1:1594437/1‑71 (MQ=255) aaaTTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTc > 1:3441981/1‑71 (MQ=255) ttAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGc > 1:3787525/1‑70 (MQ=255) aaCCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCca < 1:2568774/70‑1 (MQ=255) cAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGc < 1:1350411/71‑1 (MQ=255) cAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGc < 1:530907/71‑1 (MQ=255) aGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGc > 1:1300896/1‑70 (MQ=255) aGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCa < 1:1364410/71‑1 (MQ=255) tcGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGc < 1:1332634/65‑1 (MQ=255) gCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATc < 1:1384266/67‑1 (MQ=255) gCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:498420/70‑1 (MQ=255) ctctGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:1755118/69‑1 (MQ=255) tGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCaa < 1:717035/61‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCg > 1:3522061/1‑63 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa < 1:2888069/69‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:424125/70‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:2663366/70‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:3761061/70‑1 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACt > 1:724093/1‑71 (MQ=255) aaCTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACt > 1:2458313/1‑71 (MQ=255) cTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:2077113/71‑1 (MQ=255) cTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:1390659/71‑1 (MQ=255) cATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCAc < 1:245371/66‑1 (MQ=255) taataaATTCTATGGTTCTGCTGATTTAACCGCagag < 1:3390617/37‑1 (MQ=255) ataaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGt < 1:2888312/70‑1 (MQ=255) taaATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTgg < 1:2942122/71‑1 (MQ=255) aTTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:2464969/52‑1 (MQ=255) tcagTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAg > 1:1686334/4‑71 (MQ=255) tGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATa < 1:2987154/68‑1 (MQ=255) cagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGgag < 1:1418205/62‑1 (MQ=255) ggTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGaa < 1:550551/65‑1 (MQ=255) agTTCTGCTGATTTAACCGCTGAGAAATTCGCCAGAGCGATCCGAAATCACTGGCACg < 1:3873982/57‑1 (MQ=255) agTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCAtgtg > 1:1451689/2‑60 (MQ=255) agTTCTGCTGATTTAACCGCAGAAAAGTTCGCcac < 1:3461769/34‑1 (MQ=38) ttCTGCTGATTTAACCGCAGAGAATTTCGCCACAGCaa < 1:778613/38‑1 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGc < 1:2991690/36‑1 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:1956657/43‑1 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAAc > 1:2779023/1‑46 (MQ=255) ttCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGc < 1:3013101/71‑1 (MQ=255) ttCTGCTGATTTAACCGCAGAAAAGTTCGCCACAg > 1:1578345/1‑35 (MQ=255) ttCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTgg < 1:3736631/52‑1 (MQ=255) tCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGca > 1:2583378/1‑71 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGa < 1:3199143/41‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac > 1:1189466/1‑71 (MQ=255) gctgTTTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGc < 1:2825032/49‑1 (MQ=255) ctgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGcactg < 1:918029/70‑3 (MQ=255) ctgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTgg > 1:2072619/1‑71 (MQ=255) ctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcactg < 1:2612697/70‑3 (MQ=255) ctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcactg < 1:696136/70‑3 (MQ=255) tgATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGc > 1:2602757/1‑71 (MQ=255) tgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCa > 1:920853/1‑42 (MQ=255) gATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGgaga < 1:1378176/55‑1 (MQ=255) tttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGc < 1:410811/68‑1 (MQ=255) tttAACCGCAGAGAAGTTCGCCACAGCAATCTGAAACCACTGGCATGTGGAGAATAAGCTg > 1:464516/1‑61 (MQ=255) tttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTc < 1:3655820/71‑1 (MQ=255) ttAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCt > 1:964310/1‑71 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGga < 1:2104179/49‑1 (MQ=255) aaCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:3740812/1‑70 (MQ=255) aaCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTg > 1:3661896/1‑70 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCAc > 1:2507046/1‑42 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGa < 1:1274479/71‑1 (MQ=255) aCCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTgg > 1:2422969/1‑46 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc > 1:1126576/1‑71 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc > 1:2536068/1‑71 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:2699247/71‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGga > 1:1471115/1‑46 (MQ=255) ccGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGAc < 1:906744/71‑1 (MQ=255) cGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:3368884/71‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTg < 1:2992773/54‑1 (MQ=255) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAAt > 1:2266602/3‑47 (MQ=37) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTgg < 1:1674402/41‑1 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTgg > 1:473284/1‑71 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACg < 1:1954758/38‑1 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:2096358/1‑54 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:989865/1‑54 (MQ=255) gagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTCCACTGGCGTCTGGACGTgg > 1:3931825/1‑70 (MQ=255) agaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTgg > 1:2536499/1‑69 (MQ=255) | AAGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGG > minE/217876‑218011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |