Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 980,263 A→T 5.4% P14P (CCT→CCA) ‡ cspI ← cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE980,2630AT 5.4% 212.4 / 3.3 74P14P (CCT→CCA) ‡cspIcold shock protein
Reads supporting (aligned to +/- strand):  ref base A (35/35);  new base T (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

AATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCC  >  minE/980194‑980332
                                                                     |                                                                     
aaTTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                                                      >  1:2917685/1‑71 (MQ=255)
aaTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                                                      >  1:1779617/1‑71 (MQ=255)
 aTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                                                     >  1:1237321/1‑71 (MQ=255)
 aTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                                                     >  1:1634379/1‑71 (MQ=255)
     cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTa                                                                 <  1:2521743/71‑1 (MQ=255)
     cTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTa                                                                 <  1:3571031/71‑1 (MQ=255)
         gaAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc                                                              >  1:3664975/1‑70 (MQ=255)
          aaaTGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCa                                                            >  1:1007396/1‑71 (MQ=255)
           aaTGGACAAACACATCTTTGCTGCCATCTTTCGGAGTGATGAAACCAAAACCTTTTTCAg                                                                      <  1:494509/60‑1 (MQ=25)
            atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt                                                          <  1:987032/68‑1 (MQ=255)
             tggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAggg                                                                    >  1:1273661/3‑60 (MQ=255)
             tggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAggg                                                                    >  1:232045/3‑60 (MQ=255)
             tAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                                                         <  1:2211814/71‑1 (MQ=255)
              gcaCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCAttt                                                         >  1:2548954/3‑70 (MQ=255)
               caCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCAtttta                                                       <  1:658298/70‑3 (MQ=255)
                 cAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCAc                                                      >  1:1534400/1‑70 (MQ=255)
                 cAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACt                                                     <  1:1450160/71‑1 (MQ=255)
                 cAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACt                                                     <  1:2728011/71‑1 (MQ=255)
                  aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACTa                                                    <  1:3525091/71‑1 (MQ=255)
                   aaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTaa                                                   <  1:1430417/71‑1 (MQ=255)
                   aaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTaa                                                   <  1:3226106/71‑1 (MQ=255)
                    acacATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAggg                                                                    >  1:2235155/1‑53 (MQ=255)
                    acacATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTaaa                                                  >  1:3760251/1‑71 (MQ=255)
                      acgtCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAcc                                                <  1:2092435/68‑1 (MQ=9)
                      acgtCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAc                                                 >  1:3505348/4‑70 (MQ=9)
                      acaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAcc                                                <  1:3379269/71‑1 (MQ=255)
                              gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt                                        >  1:1066236/1‑71 (MQ=255)
                              gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt                                         <  1:2909648/70‑1 (MQ=255)
                               gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt                                       <  1:3804793/69‑1 (MQ=255)
                               gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt                                       <  1:2536224/69‑1 (MQ=255)
                                tgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                                                         <  1:2182225/52‑1 (MQ=255)
                                tgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAt                                                           <  1:531124/50‑1 (MQ=255)
                                agcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt                                       <  1:2864534/69‑1 (MQ=255)
                                  ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCAc                                                      <  1:833754/53‑1 (MQ=255)
                                  ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                                     <  1:2761567/70‑1 (MQ=255)
                                   cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                                                       <  1:3158695/35‑1 (MQ=255)
                                   cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTaa                                                                >  1:2866869/1‑42 (MQ=255)
                                   cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCa                                                       >  1:400185/1‑51 (MQ=255)
                                   cATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa                                   <  1:1984849/71‑1 (MQ=255)
                                    aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                                                      <  1:2663073/35‑1 (MQ=255)
                                    aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc                                                 <  1:2536476/56‑1 (MQ=255)
                                     tCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                                                     <  1:1740353/35‑1 (MQ=255)
                                     tCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                                                     <  1:1487346/35‑1 (MQ=255)
                                      cTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg                                      >  1:1881533/1‑65 (MQ=255)
                                       tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt                                         >  1:3310296/1‑61 (MQ=255)
                                       tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa                               >  1:1521667/1‑71 (MQ=255)
                                          ctgCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATa                             <  1:2200645/68‑1 (MQ=255)
                                          cGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATaa                            >  1:3118615/1‑71 (MQ=255)
                                          cGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATaa                            >  1:1203435/1‑71 (MQ=255)
                                            gCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt                                         <  1:3418694/56‑1 (MQ=255)
                                            gCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatt                          <  1:3671569/71‑1 (MQ=255)
                                             cGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat                           <  1:3395416/69‑1 (MQ=255)
                                             agTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAc                                                 >  1:825724/2‑47 (MQ=16)
                                                   gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                                     >  1:3460537/1‑53 (MQ=255)
                                                   gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCt                   >  1:2399905/1‑71 (MQ=255)
                                                   gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCt                   >  1:3817724/1‑71 (MQ=255)
                                                     aaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCttt                 >  1:3280759/1‑71 (MQ=255)
                                                       ccAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg               <  1:2936467/71‑1 (MQ=255)
                                                       ccAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg               <  1:800234/71‑1 (MQ=255)
                                                       ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCAtttt                                       <  1:1918042/47‑1 (MQ=255)
                                                         aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATGATTACCTTTTg               >  1:1369223/1‑69 (MQ=255)
                                                          aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt                                        >  1:590728/1‑43 (MQ=255)
                                                            aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga           >  1:769702/1‑70 (MQ=255)
                                                            aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga           >  1:3047348/1‑70 (MQ=255)
                                                            aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaag            >  1:2201983/1‑69 (MQ=255)
                                                              cTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt        <  1:2112271/71‑1 (MQ=255)
                                                              cTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtttt                                       <  1:2318751/40‑1 (MQ=16)
                                                                ttttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg               >  1:3350166/1‑62 (MQ=255)
                                                                ttttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt        >  1:3311983/1‑69 (MQ=255)
                                                                 tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                                     >  1:3499314/1‑39 (MQ=255)
                                                                 tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat                        >  1:2159221/1‑52 (MQ=255)
                                                                   tCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTGACAtt                             <  1:3601552/45‑2 (MQ=255)
                                                                    cAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGccc  >  1:1412852/1‑71 (MQ=255)
                                                                    cAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGccc  >  1:1707663/1‑71 (MQ=255)
                                                                    cAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGcc   <  1:349227/70‑1 (MQ=255)
                                                                     aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa                                   >  1:554007/1‑37 (MQ=255)
                                                                     aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt       >  1:3329426/1‑65 (MQ=255)
                                                                     aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGccc  <  1:222247/70‑1 (MQ=255)
                                                                     |                                                                     
AATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCC  >  minE/980194‑980332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑